DGGE的主成分分析(應(yīng)用spss軟件)

1、DGGE圖譜的主成分分析(1)在Excel表中，列標(biāo)為泳道，行標(biāo)為條帶，每個(gè)泳道中有條帶的表示為1，無條帶表示為0。MicrosoftExcel一dataanalysis,xls層文件(E)編輯(E)視圖插入格式(Q)工具數(shù)據(jù))窗口)幫助(H)：._；T屈二丄務(wù)Q掛-J-鑒壬血冊(cè)100%心丨3暫陽丨廠同復(fù)時(shí)包含更改.看束審閩念).島宋體12T1王U1言言手至1翌，制熬二厚寧kkA飛Z19A1ABCDEFGHIJ1條帶1條帶2條帶3條帶4條帶5條帶&條帶7條帶呂條帶92泳道11111111113泳道20011111114泳道30011111115泳道40011111116泳道5001111111

2、7泳道&0011111118泳道70111111119泳道呂00111111110泳道900111111111泳道1000111111112泳道1101111111113泳道1201111111114泳5,勺1的列刪占ZtHrl111(2丿泳將每一川中全部為0或全首叨掉，只保留部分為o和部分為1的列11117泳道1&011111111MicrosoftExcel一dataanalysis,xls噌文件(E)編輯(E)視圖插入(D格式工具(I)數(shù)據(jù))窗口辿)幫助(H)心._；T品/丄譽(yù)威/.-J7鑒壬”汀久!敢冊(cè)100%-i宋體12rh旦I言蚩手圉I翌，帑理|率率|Ei

3、p浙”aeZ24T(3)打開SPSS,將1,0數(shù)據(jù)粘貼到SPSS數(shù)據(jù)表中，最左面一列表示各個(gè)處理tel七云七1日日SPSS!Dfi-tfiEdi七Ei1ieEditYiewDataIratisforinAiialvzeG：raphsUti1itiesWindowHelp日|吧|凹1訕曲|#4|r|口並|匚軫|19:vai-UUUOlvarOOOOlV3rOOOO2var00003vsr00004varOOOOSvarOOOOS佃00007varOOOOSvarOOOOSvsrOOOl11001001001001001001001001001.1100.00.00100.001001001001

4、00二1.00.00.00.001.001.001.001.001.0011.00.00.001.001.001.001.001.00.00T1000000100100100100100100a100000010010010010010010012000010010010010010010010012000000i00i00i00i00i00i00111111.1.2000000i00i00i00i00i00i00.2-0000001001001001001001001-12-00.001001001001001001001002-00.00100100100100100100100仃3.00

5、aa1.001.001.001.001.001.00143.00.001.001.001.001.00.00.00.0030010010010010010010010000300001001001001001001001001300口口1口口1口口1口口1口口1口口1口口1口口130010010010000001001001001點(diǎn)擊工具彳欄中analysI(4)云選中數(shù)據(jù)，edatareductionfactor27titled-SPSSDataEditorFileEditViewDataTransformAnalyzeGraphsUtilitiesWindowHelpAAvarOOOOl3

6、00300300（6）-exrincicomponentsonmatrix/unrotatedfactorCorrelati;ooffactor：2IISlgnlflcRncelevels11.001-001.001.001.001.001.001.001.002100Q0Q0100Q010010010010031屈陰roo|roo|roo|roo|roo|r410000001001001001001000051JJ0.阿.阿1.001.001.001.001.001.001.81000000100100100100100100172.00_a1.001.001.001.001.001.001

7、.00a2000000100100100100100100192.000000100100100100100100T1020000001001001001001001001112.UU00i.Ooni.Oon仁則仁則仁則仁則i.Oon12200門廠IITX1FlFl13id3.00qnn5云日varOOOOZvar00003varOOOO4varOOOOSvarOOOOB300varOOOlR爲(wèi)varOOO!9洽varOOOZOvar00021tion中選擇:,-30NumberRInitialsolutionution-dlliR?Cneffl結(jié)果中36Regressi點(diǎn)擊Ok3637To1

8、0010010010020020030030030Uvsr00002varOOOOSvar00004varOOOOSvar0000&varOOOOTvarOOOOevar00009vsrOOOl10010010010010010010010000001000010010010010000000010010010010010010000100100100100100000000100100100100100100100001001001001001001001-001-001-001-001-001-001-001-001.001_001_001_001_001_001_001.-00-001-

9、001-001-001-001-001-001.001001001001001001001001001001001001001001001nnsFactorAnalyeeIX-*Correlationmatrix廠CuvariiiricematrixNumberoffactors:Display7UnrotatedfactorsolutionicreeplotvarOOO2varOOO21414innUntitled-SPSSDataEditorFileEditViewDataTransformAnalyzeGraphsUtilitiesWindowHelp昌舊直|團(tuán)訓(xùn)切I監(jiān)歯I擱罔曲圜陲庸I

10、零|釧vart)0016var00017var00018var00019var00020var00021var00022var00023var000241.00.00.00.00.00.00.00.00.0021.00.00.00.00.00.00.00.00.0031.001.00.00.00.00.00.00.00.004.00.00.00.00.00.00.00.00.005.00.00.00.00.00.00.00.00.0061.00.00.001.00.00.00.00.00.001.001.001.00.00.00.00.00.00.008.00.00.001.001.001.0

11、0.00.00.009.00.001.001.001.001.00.00.00.00101.001.001.001.00.00.00.00.00.00111.001.001.00.00.00.00.00.00.00121.001.00.00.00.00.00.00.00.00131.001.001.001.001.001.001.001.001.0014.00.00.00.00.00.00.00.00.0015.00.00.00.00.00.00.00.00.00161.001.00.00.001.00.00.00.00.0017.00.00.00.00.00.00.00.00.001Snnn

12、nnnnnnnnnnnnnnntael11.酷Output1一SPSSViewerFileEditViewinsertFormatAnalyzeGraphsUtilitiesWindowHelpQ舊直|Bl|魚團(tuán)創(chuàng)團(tuán)益I翻創(chuàng)團(tuán)丄!卜I|口|引胃|Q|-區(qū)Output-回FactorAnalysis，固Ttle=Notes由|DescriptiveStatistics討CorrelationMatrix討CommunalitiesgjTotalVarian匚eExplained討Compo仃皂ntMatrix(9)點(diǎn)擊工具欄中g(shù)raphs選擇simple，點(diǎn)擊defineFactorl-1進(jìn)入Y

13、AxixFactor2-1進(jìn)入XAxixScatterVAR000181.000.303VAR000191.000.44&VAR000201.000.520VAR000211.000.548VAR00021.000.913VAR00031.000.913VAR00041.000.913VAR000251.000.913VAR0002&1.000.913ExtractionMethod:PrincipalComponentAnalysis.CoinpcHiEnt123斗56Var00001進(jìn)入setmarkersby910111213InitialEigenlueEExtractionSumsT

14、otal%ofVarianceCumulative%Total%ofV7.49229.96929.9697.4923.1&412.&5442.S233.1642J83111.32553.9472.3089.233&3.1812.2268.90672.08&1.9367.74679.S321.4515.80685.638.9733.89289.530.8433.37492.904.7012.80395.707.3991.59897.305.2551.02098.325.159.63498.959.101.40299.361TotalVarianceExplainedsREGRfactorscos

15、lforanalysis1NJ17口nn口|七JJ|X|uu|-nr|-1M|T幽prf|41F口冏備口工J；azAiBLiV胡jjssT.xaHEiq3Aj：axIET5鬧口T忑ATP宜工。工TPR工工它-工工iK)g2L000JeaL0000Jea:Aqsja)|jenlasmLL000JeaOLOOOJEa60000Jea80000JEA000000008k00000000LV000000L00L9Lsjopejtl93tl|o.zoooojea90000Jea90000Jea000000005L辺WK00000000琳otOOOOJEA00L00L00L00LsJopEti93tl|F0000JEA000000k00k那:SIXVAZ0000JEA0000L00L00L1400L00L00L00L0Lq.od-EQq.q.B3g町血HE100L00L0000600L0000008-22F22FDO000000000000k00k00L000000000000L000000L9000000000000000000g000000000000000000V0000000000000000L00LF