如何用NCBI和uniprot數(shù)據(jù)庫查找目的蛋白的氨基酸序列或目的基因的堿基序列(mRNA序列)

1、運(yùn)用NCBI數(shù)據(jù)庫查找目的蛋白序列或目的堿基序列第1種方法1.打開NCBI官網(wǎng)，并選擇Gene菜單，如下圖。SNCBINDonalCnlriorBflectYWl叩、艸orma阻nHCBIHarneRsKMJiicaLst|AZ)AJIResourcesEhefnitaJa-BBhaaaaysal3&Sbtvvaj-EhPLARTIArnainsAStiuclLreaGenes1已出創(chuàng)帕Genecic&FAediCineGBnomea畐MapsHonHilogGene-JJ4Asemtit?BoProjettaosanieDoSYTliCnis&3QkelinvarClweCDrEeedDim

2、BinadbGaFdbVarESTGeeGenomeGGODsiaSetsGEOR-ailesGS9GTRHcmdaGEns瞰GfflLjieraiueProieinsSequenceAnairr-：TaxonomyTrainingATuborlBlBVarilontoNCBI?nlwErBintQEhnahgInfarnrHinnndvanwsdonc口nnMhc-ahhprodding3ec4e5tojgenonricrrlorriBlianCBI的陽cn|Ch陰nizitionIHCEiNewI日1切Submit3nrrnanu5cnpl3misusesDownloadTransFerN

3、CBdatalayoucampulerLrarnFindhdpdacumertsakendaclassnrwatutialiinndPopularFtK&aurcFUbMedBookahrPubMcdCc-niralFUbWetiH?0HhBLASTGeflnestjfGenePrtnei門PubChamDevelopUwAPIsandcwklihanecoCxiidaplcaiionsAnalyzeResearcliNCBIAnnovnnidUrfysitCBIi：Gil濁yiour8iaEspioreNCBresearchadC0ll3t4i3Inpia,ettSdbVarJdy2016d

4、anew1000GenomesIpnirihVarliriu1疥AAl卵H31stNCBk2在搜索欄內(nèi)輸入想要查找的蛋白名稱或基因名稱，此處以基因RSK2為例，如圖。GQnc-courcGGenomfcTstalsr3Jperps片Sori帥Reliance-SendIoFllwrfi-rGr恥日F知方CalaUDn4semjblrapfccw.Jinobiltdgents.RrWiiln-iMairj3ihuhk:nncHKCCDBErLsemSIRatSaqRe-tSeqQe-ne-fDaiorTLChrtmtEsomemara.2ailStrawmHdbonaHILMnDidyQb們閤n

5、RSK2彳百aiynAMTSoarchGofiutin3jsymtnlFindrenteddaiaiCW&a鴕SelectS-esrchresultNameiGeneIDDei匚nplimLocian13343MlMElHFFiEKfi3flb&sonisipra瑕n56KiwaA3OhrtmMGnia;.CL；5.HU-3.ISFK-133Q0T5IDG137|ig*ticibimsnifJCiQlD2311lAPKAPKIBMRX19.RSKi2014Wl12026714RSK2.SGK-suJ冏磁跑P0MR3K3?R何嘟nbosemaiostein56kinaseChnwMame；3在搜

6、索結(jié)果中查找目的蛋白，注意Description欄會(huì)標(biāo)明物種信息，以human為例，Aliases欄會(huì)列出目的蛋白的各種別名。SearchresultsPage(1of11NextLastitems:1to20or203OSamggg1diECDnlinijEdgjfggfgggjjlgpgName/GeneIDDescripticnLocalionAliasesMIMHRPS6KA3ID6197ribosomalproteinS6kinaseA3Hoedsap血占(human)ChromosomeX?NC_00DO23.11(20U9911.-pleinnenl)CLS,HU-3.ISPK-

7、1aMAPKAPK1B.MRX19.RSK:RSK2.S6K-alpha3.p9D-RSK2.pp90RSK23Q0D754.在Searchresult頁面中點(diǎn)擊你要找的蛋白名稱(Name/GeneID),進(jìn)入頁面并在頁面找到“”“NCBIReferenceSequences(RefSeq)”條目，并在其子條目中找到“mRNAandProtein(s)”，查找mRNA堿基序列點(diǎn)擊NM,查找蛋白氨基酸序列點(diǎn)擊NP,以下以查找mRNA堿基序列為例如下：亠NCBIReferenceSequences(RefSeq)日RefSeqsmaintainedindependentlyofAnnotatedG

8、enomesThesereferencesequencesexistindependentlyofgenomebuilds.Exul日inGenomic1NG_007488.1RefSeqGeneRangeDownload5001.121722GmnEimnk.F.2STASecju它nue砸恥(Gr日pliius)mRNAandPmielnk1斗5FY.TribosomalproteinS6kinasealpha-3SeeidmiitiusilDrcrtEiiisandthmiramiotsitEdlouationsforNP_D0A5?7.1點(diǎn)擊NM-004586.2進(jìn)入頁面后如下：Hom

9、osapiensribosomalproteinS6kinaseA3(RPS6KA3,mRNANCBIReferenceS&quence:NM_004586.2FASTAG閔phicsLOCUSDEFmiTIOHACCESSIONVERSIONKEWRDSSOUFJCEOFGAI-riSHKM_0045g67723bpnOllinearPEID3-Jn-2016Hojtldsapiensribdsanalprotein26kinaseA3(RPSbKAS.imKILLKM_004586BM_939339KM_9441L2KMJ：IO4586.2GI:56243494RcfScq.Hconnsa

10、piens(h.ijjn.M.,1Ikcrn口二：1口hub耳Eukaxyoia,MetazoaaChordataDCianiata,Vertcbci,Eute1estcmi;ffanuTLalia,Eutheria,Euarchontoglires.Primates;Haplorrhini.Catarrhini:Hominiilae:Homo在頁面下方會(huì)列出mRNA的序列:ORIGIN161121181241301361421481541601661721781841901961102110811141120112611321第2中方法1進(jìn)入U(xiǎn)niplot數(shù)據(jù)庫，在搜索欄中直接輸入要查找的基

11、因或蛋白名稱，如下圖:ThemiggionofUniProtistoprovidethescientificcommunitywithacomprehenaitfe,high-qualityandfreely目匚匚essiblereaourceofpi/Tiat+innc+anherlUniRefSequenceclustersUniParcSequence日r匚hiveProteomesSupportingdataLiteraturecitationsTaxanomySubcellularlocationsB&Crass-ref.databasesDiseasesKeywords吞勺TXYX

12、IIIniPrn.trlia+a2進(jìn)入以下頁面:BLASTAbgfiHACritftfA/IDnu沖旳JniProtKBresultsFilterbyicdisorja.shdwza*1Haw池id!3Q)SmSA-lYdEUnmivcd(a)Tr&W.PopularorganismsMouse僅Bflinc(3Hjr(3；iW&iLM甜khrarganumiEW2*陰站J+JMMVboni3iitolSfiUiMjlptia-3時(shí)卻鼻m15PK1.MAPKAPtClB.RK2H&rHpkfit網(wǎng)町MOEP1W54K5flW_MDU5EWwwmaiiwtelr*5Rp亦hs*Mapic-spl

13、clbj即強(qiáng)焙rad.肥竝MusmusoJiBT4QQ15349UirMnmHliwnbplniSfiMmmalp*ia-2RPHIICA?MEiPKEiPKK.RSK3HomoRipiens：(HUEjn)帀3QQWJTadXtitalih站kiritirDalplia3如乞甜曲Mipkjpkli：,RxkiMu&mutoJus733BP2S482WCflL_HUMAhHiE04Alk-ai：!ivJI：iMiiprOCOi1MAPK1EHKPfiKML,P藺WHomoiiapieiiiiiiBuivan)JbOElPZTSfllWCn3_HUhUHpnrteiMAPK-3ERKJ.PWM3

14、HomosapRfiF(Hurvan)379BR63305WKOl-MOUSilltoven-actwBtMipnrteiMl*lEricZ.Mspt.RrtanlMurrnusxKH:Mousej35BNdunMaikk2?3.3T5wukHjW亠dMimjmHA3.在搜索結(jié)果Entryname欄中會(huì)列出不同物種選項(xiàng)，且會(huì)有Proteinnames及Genenames,點(diǎn)擊所要選擇物種蛋白，進(jìn)入以下頁面。UniProtKB-P51812(KS6A3_HUMAN)Display、BLASTrQFarrnatISTAddtobasketHistoryEntryFeatureviewerProte

15、inRibosomalproteins&kinaseal|hai-3FeaturetabhGeneRPS6KA3OrganismINoneOoss-rRrencesStatus野Riaviawad-Annotationwuane;-Experimentalevidencemtpnothinlevel1Function1Eeririe/threonine-pratainkinaaethatactsdownstreamofERKfMAPKl/ERK2andMAPK3/GRK1)signalingtranstriptid門factorsCREB1,ETVl/ERaiandNR4A1/NUR.77.r

16、egulatestranslati口rithroughRPS6andE1F4survival,anddifferentiatio仃bymodulatingmTORsign-alingandrepressingpro-apciptcrti匚functionofBADphosphorylatianofCRE01andhistoneH3atSer-lO,whichresultsinthesubsequenttrans匚cptiQnmlacmitogenicsbmulation(EOFandPMAphogphgrylatBSandactivatesNR4-A1/NUR77andETvl/EFuBltr

17、ansderivedgignal,m匚indirectlyonthetranscripbonragulsticinoFseveralgenesbyphosphorylatingGSK3Eresponseto5-arumorEGFviaanmTOR-indepandentmechanismandpromotestranslationimbationbvFitoinsulinphaapriorlitesEJF4G.,enhancingEIF4QaffinityfortheEIF3camplejeandstimulabngcap-deppathwaybydirectlyphosphorylating

18、TSC2atSer-179S,whichpotentlyinhibitsTSC2abilitytoauppreswhichegulatesmTORClactivity旨ndmaypramaterapamci門-生已仃呂汁姑已mignalingindep已ndm門tl*oftheFthepro-apoptoticproteinsBADandDAPK1andsuppressingtheirpro-apoptoticfunction.Promeitestheresponsetothehepatotoxincarb口門tetrachloride匚匚同sinvolvedincellcycleregula

19、tionbyphosphaassociationwith14-3-3proteinsandpreventsitstransl口匸mCjontothenucleusandinhibitionofG2progiridiiroHfn廠enine廠irfrsuiuanrlineipqIcenrallcn廠hrnuaFfor+mrrF匚亡匚P5-m口口rlh-ncFnnTihrncin4.點(diǎn)擊頁面頁面左側(cè)的Sequence欄，并找到Sequencedatabases，其下面表格中有RefSeq，其中包含NP及NM。FunctionNoneSMiLJprcedatabasesNames&TaxonomyS

20、ubc&llularlocationStructureFamily&DomainsNames&TaxonomfySubc&BularlocarionStruGtijn?Family&DomainsSelecttheinkdesinalions-EMBLj.GenBank:DOBfiU0E315mRWJV一-mnslation：AAA91952.1.AK3L3932niRJNfl.-ran5labQn：=1AG366511.A0203933mRNA.Translator!：JAD92170.1.Di嚴(yán)erentinitiation.AL73335&JAL807772GenomicDHA.Tran

21、slation:GA405斗AL807772,AL73236S3nomicDMA.Translation:CAJ3g6S7.L.BC095301mRTJA._ranslaton:,H963J1.1.BCD95302mRTJA.Tranelatan:,AH9&3口Nl.BCD96303mRTJ4.Fnwlmti口n:AAHg53a3.1.L07599mRNA.Transition：AAC92495.1.AB102&62mRTJA.Translator!：iAC31131.1.SequenceSequenceCCDS1CCDS141&7.1.UniGene3Hs.4斗53呂7,5此處以查找mRNA

22、為例，點(diǎn)擊NM,頁面會(huì)跳轉(zhuǎn)至NCBI的查找頁面，如下:pyu-KbKZ;ppyurKZ;止；;btih-a丄phad7710/gne=/gene-SynonCLS;HU-3;ISPK-1;OPKAPK1B;HEX19;RPS6KA3P9C-RSK2;pp30RSK2;RSK;RSE2;S5K-alpha3ffORIGIN611211S12413013614214S15416016617217S1S41901961102110S1114112011261132113S1：a.ttaaccc：iu狂班垃匚狂玄玄芒乞玄玄弋丈guaaAumuaugtaaa.gg耳狂ggauatg：a已已之EEUNg3tcuttggg包gtttg：乩已ttaa已已gtattaEEuaggttEE日c：已guttta.tEcuatg：乩已g一t已l：lEaNg：a.aEEUuauairl:EaaagttGga