pBI121質(zhì)粒圖譜及載體信息

1、BI121質(zhì)粒圖譜及載體信息啟動子：35S終止子：Nos報告基因：GUS抗性基因：NptIIpBI121載體信息：LOCUS AY781296 8196 bp DNA linear SYN 10-NOV-2004DEFINITIONExpression vector pBI121_pro T-DNA region, complete sequence.ACCESSION AY781296VERSION AY781296.1GI:55418080KEYWORDS .SOURCE Expression vector pBI121_proORGANISMExpression vector pBI12

2、1_pro other sequences; artificial sequences; vectors.REFERENCE 1(bases 1 to 8196)AUTHORS Zeng,Y.H., Cao,J.W., Cai,H.Y., Chen,X.H. and Miao,L.X.TITLE Construction of the plant expression vector of the proBA gene from Bacillus subtilis mutant to proline analog and analysis of the salt tolerance of tra

3、nsgenic Arabidopsis thalianaJOURNAL UnpublishedREFERENCE 2(bases 1 to 8196)AUTHORS Zeng,Y.H., Cao,J.W., Cai,H.Y., Chen,X.H. and Miao,L.X.TITLE Direct SubmissionJOURNAL Submitted (14-OCT-2004) College of Life Science, Wuhan University, Bayi Road, Wuhan, Hubei 430072, P.R.ChinaFEATURES Location/Qualif

4、iers source 1.8196 /organism=Expression vector pBI121_pro /mol_type=other DNA /db_xref=taxon:299464 misc_feature 1.306 /note=contains T-DNA right border and Nos promoter gene 318.1112 /gene=NptII CDS 318.1112 /gene=NptII /codon_start=1 /transl_table=11 /product=neomycin phosphotransferase II /protei

5、n_id=AAV51261.1 /db_xref=GI:55418081 /translation=MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGI

6、AAPDSQRIAFYRLLDEFF terminator 1113.1365 /note=Nos promoter 2059.2859 /note=CaMV 35S misc_feature 2872.3174 /note=Arabidopsis thaliana p5csB intron in GenBank Accession Number X86778 gene 3175.4287 /gene=proB CDS 3175.4287 /gene=proB /codon_start=1 /transl_table=11 /product=gamma-glutamyl kinase /pro

7、tein_id=AAV51262.1 /db_xref=GI:55418082 /translation=MKKQRIVVKIGSSSLTNSNGSIDEAKIKEHTEAISLLKENGHEV ILITSGAVAAGFSALGYPARPVTIKGKQAAAAVGQTLLMQQYMDHLKRYGLTPAQILL TRNDFSKRERYRNAYATVMELLERGVVPIINENDSTSVEELTFGDNDMLSALVSGLIH ADQLMILTDINGLYDANPNENPDAKRFDYLTDITPELLGYAGSAGSKVGTGGMKSKFL AAQTALSLGVKVFIGTGAGREKLKV

8、ILDGKGDGTYIGDKELSTVNNTRQWIMFHSPIS GEIIIDGGAEQAMIHNGSSLLPAGVADVCGSFPKGAVVEVRGPGGIIGKGQTHYSSDE ILEAKGKRSDQLPGAKQVEVIHRNDWVNLFDEGENK misc_feature 4300.4602 /note=Arabidopsis thaliana p5csB intron in GenBank Accession Number X86778 gene 4603.5733 /gene=proA CDS 4603.5733 /gene=proA /codon_start=1 /trans

9、l_table=11 /product=glu-5-semialdehyde /protein_id=AAV51263.1 /db_xref=GI:55418083 /translation=MSEVLQKAALAKEAAAEMVMKTTAEKNEALQCIADGLRNERQLI LTENQKDIEAGQKRGLTPDIIDRLTLDEKRLLDIADAVELLIGLEDPVGESLETIQKE NGLSIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIHSNKALVSV MKRALGLSKLPIDAVQLIEDTSKETAKQLFTLNDGLDVLIP

10、RGGKNLIDLVVRESTVP VLETGAGNCHVYIDESADPDMARDVVINAKTQRPSVCNAIESLLIHEKWAEVHGRKLL NQLTEKGVELRGDQMICELEPNAKQAEEADWGAEYLAPILSVKTVHDVQEAVRHIQQY GTNHSEAILTENGEHAAYFQTAVDAAAVYHNASTRFTDGFEF gene 5758.7569 /gene=GUS CDS 5758.7569 /gene=GUS /codon_start=1 /transl_table=11 /product=beta-glucuronidase /protein_id=

11、AAV51264.1 /db_xref=GI:55418084 /translation=MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYE

12、LCVTAKSQTECDIYPLRVGIRSVAV KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGE

13、QVWNFADFATSQGILRVGGN KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ terminator 7630.7882 /note=Nos misc_feature 7883.8196 /note=T-DNA left borderORIGIN 1 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta 61 agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact 121 gacagaaccg caacg

14、attga aggagccact cagccgcggg tttctggagt ttaatgagct 181 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc 241 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc 301 aattaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 361 gggtggagag gctattcggc tatgactggg cac

15、aacagac aatcggctgc tctgatgccg 421 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 481 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 541 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 601 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag a

16、aagtatcca 661 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 721 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 781 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 841 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga 901 atatca

17、tggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 961 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 1021 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 1081 ccttctatcg ccttcttgac gagttcttct gagatcgttc aaacatttgg caataaagtt 1141 tcttaagatt gaatcctgtt g

18、ccggtcttg cgatgattat catataattt ctgttgaatt 1201 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta 1261 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa 1321 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggcc tcctgtcaat 1381 gctggcggcg gctctggtgg tggttctggt ggcggc

19、tctg agggtggtgg ctctgagggt 1441 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt 1501 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 1561 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 1621 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa

20、tggtgctact 1681 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct 1741 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct 1801 tttgtctttg gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 1861 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 1921

21、agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 1981 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 2041 aagcttgcat gcctgcagcc cacagatggt tagagaggct tacgcagcag gtctcatcaa 2101 gacgatctac ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga 2161 tgcagtcaaa agatt

22、cagga ctaactgcat caagaacaca gagaaagata tatttctcaa 2221 gatcagaagt actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt 2281 aatagagatt ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa 2341 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag 2401 tctcttacga ctcaatgaca agaagaaaat

23、 cttcgtcaac atggtggagc acgacacact 2461 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt 2521 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt 2581 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg 2641 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaag

24、atggac ccccacccac 2701 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg 2761 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc 2821 ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactctag agtttgaaat 2881 tctctgtgaa ttttttttgt tgattgatga ttgtcttcaa aaacattcct gggtttgac

25、t 2941 tttttttcat cggagcttcg tctgtgttgt tggttgatgt caattggtat ttgtattaat 3001 ccgtactttt tcaatgattg ctttcttaag ttttttaaca tttgtttttt ggcggcggag 3061 aaatctggag taatgttgaa aaacaatgac aattttgttt ctttcaatcc taaattttcg 3121 atttttctca ctacccttat ctctatttga tcgatttttt gttattgttt acagatgaaa 3181 aagcagaga

26、a ttgttgtgaa aatcgggagc agctcgctca caaacagtaa cggaagtatt 3241 gatgaagcca aaatcaagga gcatacggaa gccatttcat tattaaaaga aaacgggcat 3301 gaagtaatcc tcattacctc aggcgcggtg gcagccggtt tttccgctct cggctatccg 3361 gcgcgccccg ttacgataaa aggaaagcag gcagccgccg cggtcggcca gacgctttta 3421 atgcagcaat atatggacca ttt

27、aaaaaga tacggtctga cgccggcgca aattttatta 3481 acaagaaatg atttctcaaa aagagagcgg tacagaaatg cgtatgccac ggtaatggaa 3541 ttattggagc ggggagtcgt gccgattatt aatgagaatg attccacatc agtcgaagaa 3601 ctgacgttcg gagataatga catgctgtcg gcgcttgtga gcggcctgat tcatgcagac 3661 caattaatga ttctcacaga cattaacggg ctgtacga

28、tg ccaatccgaa tgaaaaccct 3721 gacgcaaagc ggtttgacta tttgactgac attacacctg aattgctcgg gtatgccgga 3781 tcagcgggct cgaaggtcgg caccggaggc atgaagtcaa aatttctcgc tgcccagacc 3841 gcattgtcgc tcggagttaa ggtgtttatc ggaaccggtg ccggacggga aaaactgaag 3901 gtgattttag acggtaaagg cgacggcaca tatatcggtg ataaagagct gt

29、ccaccgtc 3961 aataatacac ggcaatggat catgtttcat tctccgatat caggggaaat catcattgat 4021 gggggagcgg agcaggcgat gatccataac ggctcaagtc tccttccggc cggtgtcgcc 4081 gatgtctgcg gcagttttcc gaaaggggcc gtggtggaag tcagaggtcc cggcggcatc 4141 atcggcaaag ggcagacgca ttactcatcc gatgagattt tggaagccaa agggaagcgg 4201 ag

30、cgatcagc ttccgggcgc gaagcaggtg gaagtgattc acagaaatga ttgggtgaat 4261 ctatttgacg agggggaaaa caaatgatct agaggatccg tttgaaattc tctgtgaatt 4321 ttttttgttg attgatgatt gtcttcaaaa acattcctgg gtttgacttt tttttcatcg 4381 gagcttcgtc tgtgttgttg gttgatgtca attggtattt gtattaatcc gtactttttc 4441 aatgattgct ttcttaa

31、gtt ttttaacatt tgttttttgg cggcggagaa atctggagta 4501 atgttgaaaa acaatgacaa ttttgtttct ttcaatccta aattttcgat ttttctcact 4561 acccttatct ctatttgatc gattttttgt tattgtttac agatgagtga agtattgcaa 4621 aaagctgcct tggcaaaaga agcggctgcc gaaatggtca tgaaaacgac tgctgaaaaa 4681 aacgaagcgc tccaatgtat tgcagacgga c

32、tcagaaatg aacgccagct gattttaaca 4741 gagaatcaga aagatattga agcgggacag aaaagagggc tgacacctga cattattgac 4801 aggctgactc ttgatgaaaa acgcctgctc gatatcgcgg atgctgtaga actgttaatc 4861 ggtctggaag atcccgtcgg tgaatcattg gagaccattc aaaaagagaa tggattatcc 4921 attgaaaaaa tccgcgtgcc cctcggagta gtgggcatga tctatg

33、aagc cagaccgaat 4981 gttaccgtag acgctgccac actttgcctg aaaaccggaa acgcggttgt gctcagagga 5041 agttcgtcag ccatccacag caacaaagcg cttgtcagtg tgatgaaacg cgcactggga 5101 ctgtcaaagc ttccgattga tgccgttcag ctcatagaag atacaagcaa agaaaccgcg 5161 aaacagctct tcacattaaa tgacgggctg gatgtattga tcccgcgcgg cggcaaaaat

34、5221 ttgattgatc tggtggtcag ggaatcaacc gtacccgtgt tagaaaccgg agcgggcaac 5281 tgccacgtgt acatagatga gtcagcagat cccgatatgg caagagatgt cgtcatcaac 5341 gccaaaacac agcggccttc tgtctgcaat gcgattgaat ctcttttgat tcatgaaaaa 5401 tgggcggaag tacacggacg aaagctgctg aatcagctga cagaaaaagg ggtggagcta 5461 cgcggagatc

35、agatgatttg cgagctggag cctaatgcga aacaagccga agaggccgat 5521 tggggagcgg aatacctggc gccgattctc agtgtaaaaa cggtgcatga cgtacaggag 5581 gcggtccgcc acattcagca gtacggcaca aaccattcag aagccattct gacggaaaac 5641 ggtgagcatg ccgcttactt ccagacagcc gttgacgccg ccgccgtgta tcataatgct 5701 tccacacggt ttacagacgg ctttg

36、aattc tgaggatccc cgggtggtca gtcccttatg 5761 ttacgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc 5821 agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa 5881 agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt 5941 aattatgcgg gcaacgtctg gtatcagcgc gaagtcttta

37、 taccgaaagg ttgggcaggc 6001 cagcgtatcg tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat 6061 caggaagtga tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat 6121 gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag 6181 actatcccgc cgggaatggt gattaccgac gaaaacggca agaaaaagca gtct

38、tacttc 6241 catgatttct ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac 6301 acctgggtgg acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct 6361 gttgactggc aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa 6421 caggtggttg caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc 6481 tggc

39、aaccgg gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag 6541 tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc 6601 ctgattaacc acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggacttg 6661 cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt 6721 ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca 6781 gatgaacatg gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta 6841 ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac 6901 ggggaaactc agcaagcgc