Clitocine-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemEClitocineCat.No.:HY-118341CASNo.:105798-74-1分?式:C?H??N?O?分?量:287.23作?靶點(diǎn):Apoptosis;Bcl-2Family作?通路:Apoptosis儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:250mg/mL(870.38mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.4815mL17.4077mL34.8153mL5mM0.6963mL3.4815mL6.9631mL10mM0.3482mL1.7408mL3.4815mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存?式和期限。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥?式選擇適當(dāng)?shù)娜芙?案，配制前請先配制澄的儲備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；以下溶劑前的百分?指該溶劑在您配制終溶液中的體積占?)：1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(7.24mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(7.24mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(7.24mM);Clearsolution1/2MasterofSmallMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性Clitocine?種腺苷核苷類似物，?種有效的通透劑(readthrough)。Clitocine可誘導(dǎo)攜帶p53?義突變等位因的細(xì)胞產(chǎn)?p53蛋?。Clitocine可通過靶向Mcl-1誘導(dǎo)多藥耐藥腫瘤細(xì)胞凋亡。具有抗癌活性。體外研究Clitocine(0-0.8μM;24hours)enhancesTRAIL-lethalityininLS411NandSW620cells.Clitocine(0.2μM;36hours)significantlypotentiatesTRAIL-mediatedapoptosis[2].體內(nèi)研究Citocine(0.3-3mg/kg;s.c.;fivetimesperweek)-inducedp53inhibitsCAOV-33p53-UAA136tumorgrowthinaxenograftmodel[1].AnimalModel:nu/numice(CAOV-3p53-UAA136xenografttumors)[1]Dosage:0.3,3mg/kg(or20mg/kgonceperweek)Administration:S.c.;fivetimesperweekResult:CAOV-33p53-UAA136tumorgrowthwasinhibited.REFERENCES[1].FriesenWJ,etal.Thenucleosideanalogclitocineisapotentandefficaciousreadthroughagent.RNA.2017;23(4):567-577.[2].SunJG,etal.ClitocinepotentiatesTRAIL-mediatedapoptosisinhumancoloncancercellsbypromotingMcl-1degradation.Apoptosis.2016;21(10):1144-1157.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel