Biotin-NHS-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemEBiotinNHSCat.No.:HY-D0802CASNo.:35013-72-0分?式:C??H??N?O?S分?量:341.38作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:-20°C,sealedstorage,awayfrommoistureandlight*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)?現(xiàn)配，即刻使?。溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥3.4mg/mL(9.96mM)掃描?維碼，*"≥"meanssoluble,butsaturationunknown.運(yùn)?溶解?案計(jì)算器獲得適合您實(shí)驗(yàn)體系的溶解?案MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.9293mL14.6464mL29.2929mL5mM0.5859mL2.9293mL5.8586mL10mM---------請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)?現(xiàn)配，即刻使?。BIOLOGICALACTIVITY?物活性BiotinNHS?于制備?物素化表或多肽的氨反應(yīng)性?物素試劑。體外研究Guidelines(Followingisourrecommendedprotocol.Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds).Calculations:Forbestresults,use≥12-foldmolarexcessofbiotinfora10mg/mLproteinsolutionor≥20-foldmolarexcessofbiotinfora2mg/mLproteinsolution.1.Calculatemillimolesofbiotinreagenttoaddtothereactionfora20-foldmolarexcess:1/3www.MedChemEwww.MedChemEmLprotein×(mgprotein/mLprotein)×(mmoLprotein/mgprotein)×(20mmoLprotein/mmoLprotein)=mmoLBiotin.*20=Recommendedmolarfoldexcessofbiotinfor2mg/mlproteinsample2.Calculatemicrolitersof10mMbiotinreagentsolution(preparedinStepB.3)toaddtothereaction:mmoLBiotin×(1000000μL/L)×(L/10mmoL)=μLBiotinBiotinLabelingReaction:1.Dissolve1-10mgproteinin0.5-2.0mLPBSaccordingtothecalculationmadeinCalculations.2.Beforeuse,preparea10mMsolutionofthebiotinreagentinanorganicsolventsuchasDMSO.3.Addtheappropriatevolume(μL)of10mMbiotinreagentsolutiontotheproteinsolution.4.Incubatereactiononicefortwohoursoratroomtemperaturefor30minutes.5.ToteststhelabeledproteinbyELISAorWesternblot.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?ACSNano.2019Feb26;13(2):2501-2510.?Biomacromolecules.2019Jan14;20(1):469-477.?JStructBiol.2017Dec;200(3):360-368.?Patent.US20210038741A1.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].WojchowskiDMetal.Hybridomaproductionbysimplifiedavidin-mediatedelectrofusion.JImmunolMethods.1986Jun24;90(2):173-7.[2].NgoTTetal.Biotinyl-glucose-6-phosphatedehydrogenasepreparation,kinetics,andmodulationbyavidin.ApplBiochemBiotechnol.1982Nov;7(6):443-54.McePdfHeight2/3www.MedChemEwww.MedChemE關(guān)注MCE中國(guó)公眾號(hào)，

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