Phoyunnanin-E-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEPhoyunnaninECat.No.:HY-N9489CASNo.:886747-60-0分?式:C??H??O?分?量:482.52作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY?物活性PhoyunnaninE，分離?鐵??斛，具有抗遷移活性。PhoyunnaninE可?于癌癥研究[1]。體外研究PhoyunnaninE(0~10μM;48hours;H460cells)decreasescellmigrationthroughintegrindownregulation[1].PhoyunnaninE(0~100μM;24and48hours;H460cells)showsthattheproliferationrateoftreatedcellshavesignificantlydecreasedinadose-dependentmannerattheconcentrationof10μMat48hours[1].PhoyunnaninE(0~100μM;24hours;H460cells)inducescytotoxiceffectsattheconcentrationsfrom50to100μMandinducesasignificantincreaseinapoptoticandnecroticcellsat20~100μM[1].PhoyunnaninE(0~10μM;48hours;H460,H292andA549cells)significantlydecreasesthenumberofcellsinvadingacrossthematrixandtranswellfilterwithin24hoursinadose-dependentmanner[1].PhoyunnaninEattenuatesanchorage-independentgrowthandmigrationoflungcancerH460cells.PhoyunnaninEsignificantlyinhibitscellmigrationacrossthewoundspaceattheconcentrationsof5and10μM,at24hand48h,comparedtothenon-treatedcontrol.PhoyunnaninE(0~10μM;48hours)exhibitsasignificantdecreaseinfilopodianumbersattheprotrusionedgesofthecellsinadose-dependentmanner.PhoyunnaninEdecreasescancercellmigrationinotherhumanlungcancercells.PhoyunnaninEdecreasescancercellinvasion.PhoyunnaninEsuppressesepithelial-mesenchymaltransition(EMT).PhoyunnaninEdown-regulatesintegrinsαvandβ3andtheregulatoryproteinsincellmigration[1].WesternBlotAnalysis[1]CellLine:H460cellsConcentration:1~10μM1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEIncubationTime:48hoursResult:Decreasedcellmigrationthroughintegrindownregulation.CellProliferationAssay[1]CellLine:H460cellsConcentration:0~100μMIncubationTime:24and48hoursResult:Theproliferationrateoftreatedcellshadsignificantlydecreasedinadose-dependentmannerattheconcentrationof10μMat48h.ApoptosisAnalysis[1]CellLine:H460cellsConcentration:0~100μMIncubationTime:24hoursResult:Inducedasignificantincreaseinapoptoticandnecroticcellsat20~100μM.CellCytotoxicityAssay[1]CellLine:H460cellsConcentration:0~100μMIncubationTime:24hoursResult:Inducedcytotoxiceffectsattheconcentrationsfrom50to100μM.Immunofluorescence[1]CellLine:H460,H292andA549cellsConcentration:0~10μMIncubationTime:48hoursResult:Significantlydecreasedthenumberofcellsinvadingacrossthematrixandtranswellfilterwithin24hoursinadose-dependentmanner.REFERENCES2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE[1].PetpiroonN,etal.PhoyunnaninEinhibitsmigrationofnon-smallcelllungcancercellsviasuppressionofepithelial-to-mesenchymaltransitionandintegrinαvandintegrinβ3.BMCComplementAlternMed.2017;17(1):553.Published2017Dec29.McePdfHeightCaution:Producthasnotbeenfullyvalida