LRE1-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemELRE1Cat.No.:HY-100524CASNo.:1252362-53-0分?式:C??H??ClN?S分?量:280.78作?靶點(diǎn):AdenylateCyclase作?通路:GPCR/GProtein儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:125mg/mL(445.19mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM3.5615mL17.8075mL35.6151mL5mM0.7123mL3.5615mL7.1230mL10mM0.3562mL1.7808mL3.5615mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(7.41mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(7.41mM);ClearsolutionBIOLOGICALACTIVITY?物活性LRE1可溶性腺苷酸環(huán)化酶的特異性和變構(gòu)抑制劑。IC50&TargetAdenylylcyclase[1]體外研究LRE1bindstothebicarbonateactivatorbindingsiteandinhibitssolubleadenylylcyclase(sAC)viaauniqueallostericmechanism[1].LRE1preventssAC-dependentprocessesincellularandphysiologicalsystemsandfacilitatesexplorationofthetherapeuticpotentialofsACinhibition[1].LRE1(0.5-100μM,60minutes)inhibitsspermandmitochondrialfunctionsofsAC[1].WesternBlotAnalysis[1]CellLine:MousecaudaspermConcentration:0.5-100μMIncubationTime:60minutesResult:LRE1inhibitssACdependentspermcapacitation[1].戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?FrontCellDevBiol.2021Nov17;9:772254.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].Ramos-EspirituL,etal.DiscoveryofLRE1asaspecificandallostericinhibitorofsolubleadenylylcyclase.NatChemBiol.2016Oct;12(10):838-44.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400