MRS1845-DataSheet-生命科學(xué)試劑-MedChemExpressVIP

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEMRS1845Cat.No.:HY-103310CASNo.:544478-19-5分?式:C??H??N?O?分?量:398.41作?靶點(diǎn):CalciumChannel;CRACChannel作?通路:MembraneTransporter/IonChannel;NeuronalSignaling儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥83.33mg/mL(209.16mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.5100mL12.5499mL25.0998mL5mM0.5020mL2.5100mL5.0200mL10mM0.2510mL1.2550mL2.5100mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥5mg/mL(12.55mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥5mg/mL(12.55mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥5mg/mL(12.55mM);ClearsolutionBIOLOGICALACTIVITY?物活性MRS1845?種選擇性鈣池調(diào)控鈣離?通道(SOC)抑制劑，IC50為1.7μM[1]。MRS1845?種ORAI1抑制劑[2][3]。IC50&TargetCa2+channel[1]體外研究TreatmentwithMRS1845(10μM)virtuallydisruptstheeffectsofβ-glycerophosphateonstore-operatedCa2+entry(SOCE)[2].MRS1845(10μM)significantlydecreasesSOCEandvirtuallyabolishestheincreaseofSOCEfollowingplacentalgrowthfactor(PlGF)treatment[3].REFERENCES[1].HarperJL,etal.DihydropyridinesasinhibitorsofcapacitativecalciumentryinleukemicHL-60cells.BiochemPharmacol.2003Feb1;65(3):329-38.[2].MaK,etal.Phosphate-inducedORAI1expressionandstore-operatedCaentryinaorticsmoothmusclecells.JMolMed(Berl).2019Oct;97(10):1465-1475.[3].AbdelazeemKNM,etal.UpregulationofOrai1andSTIM1expressionaswellasstore-operatedCaentryinovarycarcinomacellsbyplacentalgrowthfactor.BiochemBiophysResCommun.2019May7;512(3):467-472.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseon