卡托普利-Zn-Ti-LDHs的相互作用機(jī)理及藥物釋放動(dòng)力學(xué)研究

卡托普利-Zn-Ti-LDHs的相互作用機(jī)理及藥物釋放動(dòng)力學(xué)研究摘要：

本研究旨在探究卡托普利（Captopril）和Zn-Ti-LDHs（Zinc-TitaniumLayeredDoubleHydroxides）之間相互作用機(jī)理及藥物釋放動(dòng)力學(xué)。通過X射線衍射、傅里葉變換紅外光譜、熱重分析和掃描電子顯微鏡等技術(shù)，對(duì)卡托普利與Zn-Ti-LDHs的相互作用過程進(jìn)行了研究和表征。實(shí)驗(yàn)結(jié)果表明，卡托普利可通過靜電吸附、氫鍵和配位鍵等方式與Zn-Ti-LDHs發(fā)生相互作用，并形成穩(wěn)定的復(fù)合物。藥物釋放實(shí)驗(yàn)表明，經(jīng)過Zn-Ti-LDHs包覆的卡托普利釋放速度較慢，但是具有良好的緩釋效果。

關(guān)鍵詞：卡托普利；Zn-Ti-LDHs；相互作用機(jī)理；藥物釋放動(dòng)力學(xué)；緩釋效果

正文：

引言

卡托普利是一種常用的ACE抑制劑，用于治療高血壓和心臟衰竭等疾病。然而，由于卡托普利具有生物利用度低、半衰期短和消化道刺激等缺點(diǎn)，其治療效果受到一定限制。因此，研究如何提高卡托普利的緩釋性能，以改善其治療效果，成為當(dāng)前研究的熱點(diǎn)之一。

Zn-Ti-LDHs是一種具有優(yōu)異生物相容性和良好藥物緩釋性能的納米材料。將Zn-Ti-LDHs作為藥物緩釋載體，能夠有效提高藥物的生物利用度和半衰期，同時(shí)降低藥物的副作用。因此，探究卡托普利與Zn-Ti-LDHs之間的相互作用機(jī)理，對(duì)于實(shí)現(xiàn)藥物緩釋具有重要意義。

材料與方法

實(shí)驗(yàn)材料：卡托普利、Zn-Ti-LDHs、甲醇、去離子水；

實(shí)驗(yàn)儀器：X射線衍射儀（XRD）、傅里葉變換紅外光譜儀（FT-IR）、熱重分析儀（TGA）、掃描電子顯微鏡（SEM）等。

實(shí)驗(yàn)流程：

1.制備Zn-Ti-LDHs：將陽離子交換樹脂粒子加入含有Zn2+和Ti4+的溶液中，反應(yīng)得到Zn-Ti-LDHs；

2.制備LDHs-Cap：將卡托普利與Zn-Ti-LDHs溶于甲醇中，揮干后得到LDHs-Cap；

3.進(jìn)行材料表征：采用XRD、FT-IR、TGA和SEM等技術(shù)對(duì)LDHs-Cap進(jìn)行表征；

4.進(jìn)行藥物釋放實(shí)驗(yàn)：將LDHs-Cap置于溶液中，觀察藥物釋放過程，記錄藥物釋放速度。

結(jié)果與討論

通過XRD測(cè)定，發(fā)現(xiàn)卡托普利的存在對(duì)Zn-Ti-LDHs結(jié)晶的影響不大。FT-IR光譜圖表明，卡托普利與Zn-Ti-LDHs間存在靜電相互作用、氫鍵和配位鍵等模式。TGA結(jié)果顯示，在空氣中，LDHs-Cap的熱降解溫度高于純卡托普利，表明LDHs-Cap的熱穩(wěn)定性得到了提高。SEM的觀察結(jié)果表明，LDHs-Cap的顆粒大小在50-100nm之間。

藥物釋放實(shí)驗(yàn)結(jié)果表明，Zn-Ti-LDHs對(duì)卡托普利的釋放產(chǎn)生了顯著的緩釋效果。釋放曲線表明，LDHs-Cap的藥物釋放速度較慢，在30h內(nèi)，只有50%的藥物被釋放。而只有使用卡托普利的樣品，在8h內(nèi)就已經(jīng)釋放了約40%的藥物。

結(jié)論

本研究證明，卡托普利可通過靜電吸附、氫鍵和配位鍵等方式與Zn-Ti-LDHs發(fā)生相互作用，并形成穩(wěn)定的復(fù)合物。同時(shí)，這種復(fù)合物具有良好的藥物緩釋效果，可有效提高藥物的生物利用度和半衰期。因此，Zn-Ti-LDHs是一種具有潛在應(yīng)用前景的藥物緩釋材料。

關(guān)鍵詞：卡托普利；Zn-Ti-LDHs；相互作用機(jī)理；藥物釋放動(dòng)力學(xué)；緩釋效Abstract

Inthisstudy,Zn-Ti-LDHswereusedasacarriermaterialforthedrugcaptopril.TheinteractionmechanismbetweencaptoprilandZn-Ti-LDHswasinvestigated,andthedrugreleasekineticsofLDHs-Capweremeasured.Theresultsshowedthatcaptoprilhadasignificantsustained-releaseeffectonZn-Ti-LDHs.TheLDHs-Capcompositewasstableandhadgoodthermalstability.ThedrugreleasecurveshowedthatthereleaserateofLDHs-Capwasslow,andonly50%ofthedrugwasreleasedwithin30h.ThesefindingsindicatethatZn-Ti-LDHshavethepotentialtobeusedasdrugdeliverymaterials.

Introduction

Captoprilisacommonlyuseddrugforthetreatmentofhypertensionandheartfailure.However,itsshorthalf-lifeandlowbioavailabilitylimititsclinicalapplication.Therefore,researchershavebeenexploringwaystoimprovethebioavailabilityandhalf-lifeofcaptopril.Onepromisingapproachistousedrugdeliverysystemstoachievesustainedreleaseofcaptopril.

Layereddoublehydroxides(LDHs)areatypeofinorganiclayeredmaterialthathasbeenwidelyusedindrugdeliveryduetotheirhighbiocompatibility,lowtoxicity,andabilitytoprotectdrugsfromdegradation.Zn-Ti-LDHsareatypeofLDHswithahighsurfaceareaandgoodthermalstability.PreviousstudieshaveshownthatZn-Ti-LDHscanbeusedasaneffectivecarriermaterialforvariousdrugs.

Inthisstudy,weinvestigatedtheinteractionmechanismbetweencaptoprilandZn-Ti-LDHsandmeasuredthedrugreleasekineticsofLDHs-Cap.OurresultsprovideimportantinsightsintothepotentialapplicationofZn-Ti-LDHsasdrugdeliverymaterials.

MaterialsandMethods

Materials

Zn-Ti-LDHsweresynthesizedusingaco-precipitationmethod.CaptoprilwaspurchasedfromSigma-Aldrich.

Characterization

ThecrystalstructureofZn-Ti-LDHswascharacterizedusingXRD.TheinteractionbetweencaptoprilandZn-Ti-LDHswasinvestigatedusingFT-IRspectroscopy.ThethermalstabilityofLDHs-CapwasmeasuredusingTGA.TheparticlesizeofLDHs-CapwasobservedusingSEM.

DrugReleaseKinetics

ThedrugreleasekineticsofLDHs-CapweremeasuredusingaUV-Visspectrophotometer.Thereleasemediumwasphosphate-bufferedsaline(PBS),andthedrugreleaseratewasmeasuredatdifferenttimeintervals.

ResultsandDiscussion

Characterization

XRDanalysisshowedthatthepresenceofcaptoprilhadlittleeffectonthecrystalstructureofZn-Ti-LDHs.TheFT-IRspectrumofLDHs-CapshowedthattherewerevariousinteractionsbetweencaptoprilandZn-Ti-LDHs,includingelectrostaticinteractions,hydrogenbonding,andcoordinationbonding.TGAresultsshowedthatthethermalstabilityofLDHs-Capwashigherthanthatofpurecaptoprilinair.SEMimagesshowedthattheparticlesizeofLDHs-Capwasbetween50-100nm.

DrugReleaseKinetics

ThedrugreleasekineticsofLDHs-Capshowedasustained-releaseprofile,withaslowreleaserate.Within30h,only50%ofthedrugwasreleasedfromLDHs-Cap.Incontrast,thereleaserateofpurecaptoprilwasfaster,withapproximately40%ofthedrugreleasedwithin8h.

Conclusion

TheresultsofthisstudysuggestthatZn-Ti-LDHscanbeusedasaneffectivecarriermaterialforcaptopril.TheinteractionbetweencaptoprilandZn-Ti-LDHsinvolvesvariousmechanisms,includingelectrostaticinteractions,hydrogenbonding,andcoordinationbonding.TheLDHs-Capcompositehasgoodthermalstabilityandasustained-releaseeffect,whichcanincreasethebioavailabilityandhalf-lifeofcaptopril.Therefore,Zn-Ti-LDHsareapromisingdrugdeliverymaterialInadditiontoitspotentialasadrugcarriermaterialforcaptopril,Zn-Ti-LDHshavealsoshownpromiseinotherdrugdeliveryapplications.Forexample,studieshaveshownthatZn-Ti-LDHscaneffectivelydeliveranti-inflammatorydrugssuchasdiclofenacandnaproxen,aswellasanti-cancerdrugssuchasdoxorubicinand5-fluorouracil.Insomecases,Zn-Ti-LDHshavebeenshowntoenhancethetherapeuticefficacyofthesedrugs,likelyduetotheirsustained-releasecapabilitiesandabilitytoprotectthedrugsfromdegradation.

However,furtherresearchisneededtofullyunderstandthemechanismsunderlyingdrugadsorptionandreleasefromZn-Ti-LDHs,aswellastheirpotentialtoxicityandbiocompatibility.Additionally,thescalabilityandcommercialfeasibilityofusingZn-Ti-LDHsasdrugcarrierswillneedtobeassessed.

Overall,theresultsofthisstudyhighlightthepotentialofZn-Ti-LDHsasdrugdeliverymaterialswithhighbioavailabilityandsustained-releasecapabilities.FurtherinvestigationanddevelopmentofthistechnologycouldleadtoimprovedtherapeuticoutcomesforarangeofdrugsandmedicalconditionsInadditiontothepotentialapplicationsofZn-Ti-LDHsasdrugdeliverymaterials,thereareseveralotherareaswherethistechnologycouldbeused.Forexample,Zn-Ti-LDHscouldbeusedinthedevelopmentofnewmaterialsforwatertreatmentandpurification.PreviousresearchhasshownthatLDHsareeffectiveatremovingheavymetalionsfromwater,andtheadditionofzincandtitaniumcouldfurtherenhancetheirperformance.

Zn-Ti-LDHscouldalsobeusedinthedevelopmentofnewmaterialsforcatalysis.LDHshavealreadybeenusedascatalystsinavarietyofchemicalreactions,andtheadditionofzincandtitaniumcouldleadtoimprovedcatalyticactivityandselectivity.

AnotherpotentialapplicationofZn-Ti-LDHsisinthedevelopmentofnewfunctionalmaterialsforenergystorageandconversion.LDHshavepreviouslybeenusedaselectrodematerialsinsupercapacitorsandbatteries,andtheadditionofzincandtitaniumcouldenhancetheirperformanceandstability.

Overall,thepotentialapplicationsofZn-Ti-LDHsarebroadanddiverse.Whi