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AcetobacterMedium(醋酸菌培養(yǎng)基)Glucose(葡萄糖)100gYeasstextract酵母膏)10gCaCO320gAgar(瓊脂)15gDistilledwater(蒸餾水)1000mlAdjust(調(diào))pHto6.8適用范圍:惡臭醋酸桿菌混濁變種NutrientAgar(營養(yǎng)肉汁瓊脂)Pepton(蛋白胨)5gBeefextract(牛肉膏)30gNaCl5gAgar(瓊脂)15gDistilledwater(蒸餾水)Adjust(調(diào))pHto7.0-7.2[Note]:WhencultivationofBacillus,5mgoftoMnSO4.H2Omaybeadded.Itisfavorabletopromotesporeformation.適用范圍:產(chǎn)氣氣桿菌、糞產(chǎn)堿桿菌、蠟狀芽抱桿菌、蠟狀芽抱桿菌蕈狀變種、地衣形芽抱桿菌、巨大芽抱桿菌、多粘芽抱桿菌、塵埃芽抱桿菌、短小芽抱桿菌、嗜熱脂肪芽抱桿菌、枯草芽抱桿菌、枯草芽抱桿菌深黑變種、蘇云金芽抱桿菌、蘇云金芽抱桿菌蠟螟亞種(青蟲菌)、蘇云金芽抱桿菌戈?duì)査沟伦兎N、蘇云金芽抱桿菌猝倒亞種、產(chǎn)氨短桿菌、黃色短桿菌、谷氨酸棒狀桿菌、北京棒桿菌、大腸埃希氏菌(大腸桿菌、銅綠假單胞菌(綠膿桿菌X凸形假單胞桿菌、熒光假單胞菌、彎曲假單胞菌、惡臭假單胞菌、假單胞桿菌、藤黃八疊球菌、亞黃八疊球菌、尿素八疊球菌、金黃色葡萄球菌、運(yùn)動發(fā)酵單抱菌AzotobacterMedium(固氮菌培養(yǎng)基)KH2PO40.2gK2HPO40.8gMgSO4.7H2O0.2gCaSO4.2H2O0.1gNa2MoO4.2H2OTrace(微量)Yeastaxtract(酵母膏)0.5gMannitol(甘露醇)20gFeCl3Tract(微量)Distilledwater(蒸餾水)1000mlAgar(瓊脂)15gAdjust(調(diào))pHto7.2適用范圍:固氮菌、膠質(zhì)芽抱桿菌CornMealMedium(玉米粉培養(yǎng)基)Maizeflour(玉米粉)5gPeptone(蛋白胨)0.1gGlucose(葡萄糖)1gTapwater(自來水)1000ml[Note]:Boilthemixtureinautoclaveat121°Cfor1hr.distributethemediuminto18x18mmtubes,eachcontains10mloftheliquid,thenautoclaveat121Cfor1hr.again(15磅蒸煮1小時(shí),分裝入18x18毫米試管,每管深度達(dá)6厘米。15磅再次滅菌15小時(shí)。)Lactic-bacteriaMediumI(乳酸菌培養(yǎng)基I)Yeastextract(酵母膏)7.5gPeptone(蛋白胨)7.5gGlucose(葡萄糖)10gKH2PO42gTomatojuice(西紅柿汁)100mlTween吐溫)800.5mlDistilledwater(蒸餾水)900mlpH7.0適用范圍:植物乳桿菌(胚芽乳桿菌)、嗜熱乳酸鏈球菌Lactic-bacteriaMidiumII(乳酸菌培養(yǎng)基II)Lacto-caseinpeptone(乳酪蛋白胨)10gBeefextract(蛋白胨)10gYeastextract酵母膏)5gGlucose(葡萄糖)5gTween(吐溫)801gK2HPO42gNa-acetate(醋酸鈉)5gDiaminecitrate(檸檬酸二胺)2gMgSO4.7H2O0.2gMnSO4.H2O0.05gDistilledwater(蒸餾水)1000mpH6.5-6.8適用范圍:植物乳桿菌(胚芽乳桿菌)PeotoneGlucoseYeastextractMediumPGY(蛋白胨、酵母膏、葡萄糖培養(yǎng)基)Peptone(蛋白胨)10gYeastextract(酵母膏)5gGlucose(葡萄糖)1gDistilledwater(蒸餾水)1LGlycerolAgar(甘油瓊脂)Peptone(蛋白胨)5gBeefextract(酵母膏)3gGlycerol(甘油)20gTopwater(自來水)1000mlAgar(瓊脂)15gpH7.0-7.2Rhizobiummedium(根瘤菌培養(yǎng)基)AS9Yeasteztract(酵母膏)1gSoileztract(土壤浸提液)200mlMannitol(甘露醇)10gAgar(瓊脂)15gDistilledwater(蒸餾水)800mlpH7.2[Note]:Soilextract:Suspend50gfinelyanddriedgardonsoilin200mloftapwater.Autoclaveat121°Cfor1hr.Decantthroughcotton-cloth,fillerthoughpaper,makeupvolumeto200ml.Resterilizefor20minutesat121C,thenmixedwithothringredientsanddistributed.(土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小時(shí),經(jīng)濾紙過濾后加水補(bǔ)足到200毫升。)適用范圍:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌MannitolAgar(甘露醇瓊脂)Yeastextract(酵母膏)5gPeptone(蛋白胨)3gMannitol(甘露醇)25gAgar(瓊脂)15gDistilledwater(蒸餾水)1000ml11.GlucoseAsparagine(葡萄糖、天門冬素瓊脂)Glucose(葡萄糖)10gAsparagine(天門冬素)0.5gK2HPO40.5gWater(水)1000mlpH7.2-7.4適用范圍:刺抱小單抱菌絳紅變種、紫色小單抱菌(絳紅小單抱菌)12.Gause'sSyntheticA高氏合成一號瓊脂)KNO31gSolublestarch(可溶性淀粉)20gK2HPO40.5gMgSO4.7H2O0.5gNaCl0.5gFeSO40.01gAgar(瓊脂)20gwater(水)1000mlpH7.2-7.4適用范圍:刺抱小單抱菌絳紅變種、紫色小單抱菌(絳紅小單抱菌)、白黃鏈霉菌、白色鏈霉菌、抗生鏈霉菌、雙重輪絲鏈霉菌、產(chǎn)色鏈霉菌、燼灰鏈霉菌、天藍(lán)色鏈霉菌、滅蚊鏈霉菌、紅霉素鏈霉菌、青色鏈霉菌、球抱鏈霉菌、淺灰鏈霉菌、灰色鏈霉菌、吸水鏈霉菌、淡紫灰鏈霉菌、黃色長抱鏈霉菌、藤黃色鏈霉菌、細(xì)黃鏈霉菌、黑化鏈霉菌、玫瑰色鏈霉菌、華美鏈霉菌、嗜熱鏈霉菌、委內(nèi)瑞拉鏈霉菌、紫色直絲鏈霉菌、紫色鏈霉菌、綠色鏈霉菌13.WortAgar(麥芽汁瓊脂)Dilutetheworld(withouthop)to12Brix.Add15gagarinto1000mlofthedilutedword..Melttheagarbyheating,thendistributethemediumintotubes.Autoclaveat110for30minutes.將發(fā)酵啤酒的原料(未加酒花),稀釋至12柏林,加瓊脂15克,溶化后分裝。15磅滅菌30分鐘。)適用范圍:克魯斯假絲酵母、郎比可假絲酵母、解脂假絲酵母、馬其頓假絲酵母、擬熱帶假絲酵母、粗壯假絲酵母、皺褶假絲酵母、熱帶假絲酵母、產(chǎn)骯假絲酵母、阿舒假囊酵母、白地霉、果香地霉、地霉屬、異常漢遜酵母、異常漢遜酵母變種、阿拉伯糖醇漢遜酵母、施氏漢遜酵母、菅囊酵母、伯頓畢赤酵母、膜醭畢赤酵母、粘紅酵母、小紅酵母、膠紅酵母、深紅酵母、卡爾斯伯酵母、釀酒酵母、椰園釀酒酵母、發(fā)酵性酵母、洛格酵母、羅斯酵母、魯氏酵母多形魯氏酵母、威爾酵母、酵母、路德類酵母、栗酒裂殖酵母、擲抱酵母、貝雷絲抱酵母、皮狀絲抱酵母、糙抱曲霉、無殼曲霉、鮮橙曲霉、紅曲霉、紫紅曲霉、紅色紅曲霉、紅曲霉菌14.PotatoDextroseAgarPDA(馬鈴薯、葡萄糖瓊脂)Potatoextract(馬鈴薯汁)1000mlDextrose(glucose)(葡萄糖)20gAgar(瓊脂)20g[Note]:Potatoextract:Slicepotatoesthin.Add1000mlofwater,immediatelytopreventOxidtionofjuiceandboiluntilsoft.(approximately30min.).Filterthroughcotton-cloth.Autoclaveat115Cfor20minutes.(注:取去皮馬鈴薯200克,切成小塊,加水1000毫升煮沸30分鐘,濾去馬鈴薯塊,將濾液補(bǔ)足至1000毫升,加葡萄糖20克,瓊脂15克,溶化后分裝,15磅滅菌30分鐘。)適用范圍:酵米面假單胞菌(酵米面黃桿菌)、白色鏈霉菌、燼灰鏈霉菌、青色鏈霉菌、球抱鏈霉菌、灰色鏈霉菌、龜裂鏈霉菌、傘枝梨頭霉、雅致放射毛霉、棒曲霉、米曲霉、出芽短梗霉、白僵霉、灰葡萄抱、頂頭抱霉、巴西毛殼、長刺毛殼、橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、束狀刺盤抱、新月彎抱霉、奇異翅抱殼、地生翅抱殼、串珠鐮抱、尖鐮抱、盤長抱菌(魯保一號)、銀白楊盤長抱、玉蜀黍長蠕抱、深黃被抱霉、小被抱霉、多頭被抱、拉曼被抱霉、葡萄色被抱霉、生香毛霉、卷枝毛霉、兩型抱毛霉、直立毛霉、球抱毛霉、絲球毛霉、大毛霉、多型抱毛霉、總狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬青霉、擬青霉、球形阜抱、白腐菌、少根根霉、華根霉、科恩根霉、戴爾根霉、日本根霉、爪哇根霉、米根霉、點(diǎn)頭根霉、綠穗霉、簇抱匍柄霉、雅致枝霉、康寧木霉、綠色木霉、黃萎輪枝抱、大麗花輪枝抱15.CzapeksAgar(察氏瓊脂)Sucrose(蔗糖)30gNaNO33gMgSO4.7H2O0.5gKCl0.5gFeSO4.7H2O0.01gK2HPO41gAgar(瓊脂)13gDistilledwater(蒸餾水)1000mlpH7.2適用范圍:紅霉素鏈霉菌、洋蔥曲霉、金頭曲霉、泡盛曲霉、泡盛曲霉煙色變種、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、無花果曲霉、黃柄曲霉、黃曲霉、煙曲霉、構(gòu)巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、醬油曲霉、土曲霉、宇佐美曲霉、焦曲霉、雜色曲霉、溫特曲霉、阿達(dá)青霉、產(chǎn)黃青霉、黃綠青霉、桔青霉、頂青霉、園弧青霉、繩狀青霉、灰黃青霉、島青霉、淡紫青霉、特異青霉、赭綠青霉、產(chǎn)紫青霉、婁地青霉、小刺青霉、蕁麻青霉、16.ConcentratedSucroseCzapek's(濃糖察氏瓊脂)Sucrose(蔗糖)200gNaNO33gMgSO4.7H2O0.5gKCl0.5gFeSO4.4H2O0.01gK2HPO41gAgar(瓊脂)13gDistilledwater(蒸餾水)1000ml適用范圍:謝瓦曲霉SyntheticPotatoMedium(綜合馬鈴薯培養(yǎng)基)20%Potatoextract(馬鈴薯汁)1LGlucose(葡萄糖)20gKH2PO43gMgSO4.7H2O1.5gVitanibB1(硫胺素)Trace(微量)Agar(瓊脂)15gpH6適用范圍:米曲霉、醬油曲霉、棲土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄抱、頂頭抱霉、巴西毛客、長刺毛殼、球毛殼、橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、多主枝抱、束狀刺盤抱、葫蘆科刺盤抱、新月彎抱霉、奇異翅抱殼、地生翅抱殼、串珠鐮抱、蝕脈鐮抱霉、盤長抱菌(魯保一號)、銀白楊盤長抱、瓶毛殼、金龜子綠僵菌、生香毛霉、卷枝毛霉、兩型抱毛霉、直立毛霉、球抱毛霉、絲球毛霉、大毛霉、多型抱毛霉、總狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬青霉、淡紫色擬青霉、擬青霉、白腐菌、少根根霉、根霉屬的菌、華根霉、科恩根霉、戴爾根霉、臺灣根霉、日本根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、點(diǎn)頭根霉、三寶壟根霉、根霉屬的種、綠穗霉、康寧木霉、木素木霉、擬康氏木霉、木霉、粉紅木霉、綠色木霉、黃萎輪枝抱、大麗花輪枝抱、雙抱蘑菇、大肥菇、發(fā)光假蜜環(huán)菌、木耳、皺木耳、毛木耳、大禿馬勃、長根菇、金針菇、棘托竹蓀、短裙竹蓀、短裙竹蓀東北變種(暫定)、長裙竹蓀、白鬼筆、無裙竹蓀、寬鱗大孔菌、凸圓靈芝(白芝、紫芝、靈芝(紅芝,赤芝,日本靈芝,韓國靈芝)、灰樹花、猴頭菌、香菇、粗毛斗菇、潔麗香菇、豹皮菇、玉蕈、真姬菇、安絡(luò)小皮傘菌、鬼毛針、黑柄皮傘菌、尖頂羊肚菌、圓錐羊肚菌、高羊肚菌、黑脈羊肚菌、羊肚菌、滑菇、光帽黃傘、尖鱗黃傘、金頂側(cè)耳、榆黃磨、金頂磨、白黃側(cè)耳、紫抱平菇、姬菇、裂皮側(cè)耳、擬囊體側(cè)耳(鮑魚菇)、真線側(cè)耳短抱變種、真線側(cè)耳、扇形平菇、扁形平菇、佛羅里達(dá)無抱子側(cè)耳、佛羅里達(dá)側(cè)耳、蠔菇、糙皮側(cè)耳、平菇、貝形側(cè)耳、鷹翅菌、肺形側(cè)耳、柳樹菌、樹窩、扁柄側(cè)耳、美味側(cè)耳、紫抱側(cè)耳、粉褶側(cè)耳、粉紅側(cè)耳、鳳尾菇、漏斗狀側(cè)耳、榆干側(cè)耳、大榆磨、側(cè)耳、茯苓、黑菇、煙色紅菇、裂褶菌、雞縱、金耳、黃木耳、銀耳、白木耳、紫晶口磨、銀絲菇、絲蓋小包腳菇、草菇CornMealMedium(玉米粉培養(yǎng)基)Mixcornmealwithwater[20:100(W/V)]well.Distributethemixtureinto100mlbottles,eachbottlecontainsabout30mlofthemixture.Autoclaveat121°Cfor30minutes.(20%玉米粉裝瓶,15磅滅菌30分鐘。)FilterPaperMedium(濾紙培養(yǎng)基)(NH4)2SO41gKH2PO41gMgSO4.7H2O0.7gNaCl0.5gDistilledwater(蒸餾水)1000mlAstripoffilterpaper(濾紙條)6x1cm(厘米)SoybeanCakeMealAgar(黃豆餅粉瓊脂)10%infusionofsoybeancakemeal(10%黃豆餅粉浸汁)1000mlGlucose(葡萄糖)10gNaCl2.5gSawdust,Wheat-branMedium(木屑、麩皮培養(yǎng)基)Sawdustofbroadleaftree75gWheatbran25gPreparation:Mixthesetwoingredientswell.Addappropriatewaterandblend(theamountofwateraddedmaybebecidedbykneadingwithfingers).Fillthemixtureintobroadmouthofbottles.Stickaholeonthefilling,putthecottonpulginbottlemouth.殼斗科植物或闊葉樹的木屑75克麩皮25克制法:加水適量充分混勻,水量以手捏時(shí)指間有水珠為宜,裝瓶并壓緊,在中心處扎一小洞,堵上棉塞,20磅滅菌1小時(shí)。PineBlockorPineSowdustMedium(松木條或松木屑培養(yǎng)基)Cut1021cmpinebiocksandimmersethemin1-2%sucrosesolution.Alowthebiocksfullyabsorbingsugersolution.Fillthebiocksintobroadmonthofbottle.Parkthebottlewithpaper.Autoclaveat20lbsfor1hr.(將10x2x1厘米的松木條浸泡在1-2%的蔗糖水中。使木條吸足糖水,裝瓶不宜過緊,用紙包好,20磅滅菌1小時(shí)。)PineSawdustMediumPineSawdust(松木屑)75%Ricebran(米糠)23-24%Socrose(蔗糖)1-2%Water(appropriate)水(適量)Autoclaveat20lbsfor1hr.(20磅滅菌1小時(shí))RiceStraw.DungMedium稻草、馬糞培養(yǎng)基)Driedhorsedung(orcowdung)(干馬糞或牛糞)65%Diedstraw(干稻草)35%Urea(尿素)0.5%Calciumsuperhosphate(過磷酸鈣)0.7%Gypsumpowder(石膏粉)1%Ammoniumsulphate(硫酸銨)0.3%[Note]:Addappropriatewater,pilethemupandcausethemfermentation.(水適量進(jìn)行堆積發(fā)酵)StrawRiceBranMedium(稻草、米糠培養(yǎng)基)Driedstraw(干稻草)75-80%Ricebran(米糠)20-25%[Note]:Selectnomouldystrawandcutthemabout3cminlength.Immersetheminfreshwaterfor12hr.Allowthestrawfully(theamountofwateraddedmaybedecidedbykneadingwithfingers.Itisrightwhenadropofwaterappearedbetweenfingers).([注]:選無霉稻草,切成長約3厘米的小段,用清水浸泡12小時(shí),使充分吸水,然后傾去水,加米糠和水,水量以用手捏時(shí)指間有水珠為宜。)RiceMedium(米飯培養(yǎng)基)Fill10gofriceintoa50mlofErlenmeyerflask.Add20-30mlwater.Autoclaveat121Cfor30minutes(orfill2gofriceintoa15x150mmtube).Add6mlofwater.Autoclaveat121Cfor30minutes.(取大米10克,入50毫升椎形瓶中,水20-30毫升于15磅滅菌30分鐘即成,或取大米2克,裝入15x150毫米試管中,加水6毫升,于15磅滅菌30分鐘。)FilterPaperMedium(濾紙條培養(yǎng)基)(NH4)2SO41gKH2PO41gMgSO4.7H2O0.5gYeastExtract酵母膏)0.1gDistilledWater蒸餾水)1000mlStripoffilterpaper1x7cm(濾紙條1x7厘米)[Note]:Siddolvetheaboveingredients.Distributetheliqidmediumintotubes(eachtubecontains1mlofthebroth).Autoclaveat121°Cfor30minutes.Thenputoneortwosterilizedstripsoffilterpaperintoatubeaseptically.(將上述溶液分配成溶液后分裝入試管,每管1毫升,15磅滅菌30分鐘。再將滅菌的濾紙條(1條或2條)投入試管中即好使用。)YeastExtract-MediumExtractAgarI酵母膏、麥芽膏瓊脂)Yeastextract(酵母膏)10gMallextract(麥芽膏)10gGlucose(葡萄糖)4gDistilledwater(蒸餾水)1000mlAgar(瓊脂)20gpH7.0適用范圍:紅霉素鏈霉菌YeastExtractPeptone(酵母膏、蛋白胨瓊脂)Yeastextract(酵母膏)1gMulti-peptone(多蛋白胨)2gBeefextract(牛肉膏)1gGlucose(葡萄糖)10gAgar(瓊脂)20gDistilledwater(蒸餾水)1LpH7.0GlucoseYeastExtractStarchAgar(葡萄糖、酵母、淀粉瓊脂)Glucose(葡萄糖)10gYeastextract(酵母膏)10gStarch(淀粉)10gNaCl5gCaCO33gAgar(瓊脂)20gDistilledwater(蒸餾水)1LpH7.0WheatBranMedium(麩皮培養(yǎng)基)Wheatbran(麩皮)36g(NH4)2HPO410gK2HPO40.2gMgSO4。7H2O0.1gAgar(瓊脂)20gDistilledwater(蒸餾水)1LpH7.0YeastSporulationMedium(酵母菌產(chǎn)生子囊抱子培養(yǎng)基)(1)KleynMediumKH2PO40.012%K2HPO40.02%Sodiumacetate(醋酸鈉)0.5%Glucose(葡萄糖)0.062%NaCl0.062%Peptone(蛋白胨)0.25%Biotin(生物素)2Mg%Aga瓊脂)2%Traceelementsolution(混合鹽類)1ml/100ml[Note]:Traceelementsolution(混合鹽類溶液):MgSO4.7H2O0.4%,NaCl0.4%,CuSO4.5H2O0.002%,MnSO4.4H2O0.2%,FeSO4.4H2O0.2%(2)GorodkowaMediumGlucose(葡萄糖)0.1%Peptone(蛋白胨)1%NaCl0.5%Agar(瓊脂)2%(3)McClaryMediumGlucose(葡萄糖)0.1%KCl0.18%Yeastjuice酵母汁)0.25%Sodiumactate(醋酸鈉)0.82%Agar(瓊脂)1.5%BPYMedium(BPY培養(yǎng)基)Beefextract(牛肉膏)5gPrptone(蛋白胨)10gNaCl5gYeastextract酵母膏)5gGlucose(葡萄糖)5gAgar(瓊脂)1-2%Distilled(蒸餾水)1000mlpH7.0適用范圍:大腸埃希氏菌(大腸桿菌)、惡臭假單胞菌LBMedium(LB培養(yǎng)基)Yeastextact酵母膏)5gPeptone(蛋白胨)10gNaCl10gAgar(瓊脂)1-2%Distilledwater(蒸餾水)1000mlpH7.0適用范圍:大腸埃希氏菌(大腸桿菌)PenssayBroth(Penssay肉湯)Beefextract(牛肉膏)1.5gYeastextract酵母膏)1.5gTryptone(胰蛋白胨)5gNaCl3.5gGlucose(葡萄糖)1gK2HPO4.3H2O4.8gDistilledwater(蒸餾水)1000mlpH7.2BlondAgar(血瓊脂培養(yǎng)基)Peptone(蛋白胨)10gGlucose(葡萄糖)2gNaCl5gNaH2PO42.5gSodiumcitrate(檸檬酸鈉)5gBraininfusion(腦浸汁)800mlPigheartinfusion(豬心浸)200mlSleepBlood(羊血)10%pH7.2[Note]:Preparationofpigbrainandpigheartinfusion:(1)Smashup100gfreshpigheart(withoutfatandbloodvessel).Adddistilledwaterupto1000ml;(2)Cut200gfreshpigbrain(withoutbloodvessel).Adddistilledwaterupto1000ml;(3)Keepthetwoinfusionsat50°Cfor1hr,thenboilfor15minutes;(4)Coolandfiltratethroughgauze.[注]豬心和豬腦浸汁法:(1)新鮮豬心除去心頭和脂肪,攪碎100克加蒸餾水至1000毫升;(2)新鮮豬腦除去血管,取200克加蒸餾水至1000毫升;(3)50C呆溫1小時(shí),然后煮開15分鐘;(4)冷卻后用紗布過濾備用。Cellulose-decomposingBacteriaSyntheticMedium(纖維細(xì)菌合成培養(yǎng)基)NaCl6gMgSO4.7H2O0.1gKH2PO40.5gCaCl20.1gK2HPO42.0gYeastextract酵母膏)1.0g(NH4)2SO42.0gDistilledwater(蒸餾水)1000mlFilterpaperstrip:onestrip(濾紙條:一片)pH7.0-7.4[Note]:So;idmediumforcellulose-decomposingbacteria:CarboxymethylCellulose2%Agar2%.(斜面培養(yǎng)基去濾紙條用2%瓊脂及2%羧甲基纖維素鈉CMCNa)ClostridiumPasreurianumSyntheticMedium(巴氏梭狀芽抱桿菌合成培養(yǎng)基)Glucose(葡萄糖)1%MnSO40.001%KH2PO40.05%FeSO40.001%K2HPO40.05%Yeastextract(酵母膏)0.1%MgSO4.7H2O0.02%Peptone(蛋白胨)0.01%NaCl0.001%CaCO30.5%Distillewater(蒸餾水)100%pH7.0YeastExtract-MediumExtractAgarII(酵母膏、麥芽膏瓊脂II)Yeastextract(酵母膏)4gMallextract(麥芽膏)10gGlucose(葡萄糖)4gAgar(瓊脂)17gDistilledwater(蒸餾)1000mlpH7.3OatemealAgar(燕麥粉瓊脂)Oatemeal(infusion)[燕麥粉浸液]20gAgar(瓊脂)18%Traceelementsolution(跡量鹽溶液)1mlDistilledwaterupto(用蒸餾水補(bǔ)充至1000毫升)1000mlpH7.2[Note]:Traceelementsolution(跡量鹽溶液):FeSO4.7H2O0.1gMnCl2.4H2O0.1gZnSO4.7H2O0.1gDistilledwater(蒸餾水)100mlCalciumMalateAgar(蘋果酸鈣瓊脂)Caliummalate(蘋果酸鈣)10gK2HPO40.5gNH4C10.5gGlycerol(甘油)10gAgar(瓊脂)15gDistilledwater(蒸餾水)1000mlThiobacillusFerrooxidansMedium(氧化亞鐵硫桿菌培養(yǎng)基)(NH4)2SO40.15gKH2PO40.05gKCl0.05gMgSO4.7H2O0.5gCa(NO3)2.4H2O0.01gFeSO4.7H2O50gDistilledwater(蒸餾水)1000mlpH2.0ThiobacillusThiooxidansMedium氧化硫硫桿菌培養(yǎng)基)(NH4)2SO40.3gKH2PO43-4gCaCl2(anhydrous)[無水]0.25gMgSO4.7H2O0.5gFeSO4.7H2O0.001gDistilledwater(蒸餾水)1000mlpH3.5-4PlasmaSubstituteMedium(代血漿培養(yǎng)基)Sucrose(蔗糖)12.5-15%Peptone(蛋白胨)0.5%KH2PO40.03%Na2HPO4.12H2O0.14%pH7.0-7.2SkimMilkMedium(脫脂牛奶培養(yǎng)基)ATCC183脫脂牛奶100g去離子水1000ml適用范圍:保加利亞乳桿菌、嗜熱乳酸鏈球菌PeaAgar(豌豆瓊脂)Glucose(葡萄糖)1%Peptone(蛋白胨)0.5%NaCl0.5%Agar(瓊脂)2.0%Peainfusion(countedbyNH2)[豌豆浸汁(以NH2計(jì))]12-14mg/100pH(beforesterilization)[消前]7.2-7.5[Note]:Preparetionofpeainfusion(豌豆浸汁的制法):Preparetion900gofpea.Putthemintoa5Lbottle,andadd3Loftapwater.AdjustpHto4.3-4.5byusingofsulphuricacid.Add30mlofchloroformforantisepsis.Plugwithrubberstopperandbindupthebottlemouthwithgaugeinordertoavoidthatthestopperfalloffduetogaspressure.Placethebottleat37°Cfor7-10days.Filtertheinfusion,thenboilthefiltratetoescapethechloroform.FilteragainDistributionandautoclaveat120Cfor30minutes.Storeatcoolcontition.(稱取白豌豆900克,置于5公升廣口瓶中,加自來水3公升,用硫酸調(diào)pH至4.3-4.5,再加氯仿30毫升(防腐),加膠塞并用紗布扎口(防止沖脫),放37C恒溫室7-10天。取出用濾紙過濾,濾液煮沸20分鐘,以除氯仿,并再次以濾紙過濾,除沉淀。濾液分裝,于120C30分鐘滅菌。后冷藏備用。)Thestandardqualityofpeainfusionasfollowing(豌豆浸汁質(zhì)量標(biāo)準(zhǔn)):Aminonitrogen(氨基酸):160-200mg400mlSolublephosphoruse(溶磷):400-600pgmiColor(色澤):auratus,transparent(金黃、透明)WheatBranMedium(麥麩瓊脂)Wheatbran(麥麩)3.6%(NH4)2HPO40.3%K2HPO40.02%MgSO4.7H2O0.01%Agar(瓊脂)2%pH7.0適用范圍:金霉素鏈霉菌、紅霉素鏈霉菌GlucoseYeastExtractAgar葡萄糖、酵母膏瓊脂)Glucose(葡萄糖)10gYeastextract酵母膏)10gWater(水)1000mlAgar(瓊脂)15gpH7.2MMNMedium(MMN培養(yǎng)基)CaCl20.05gMgSO40.15gNaCl0.025gFeCl3(1%)1.2mlKH2PO40.5gVitamibB1(硫胺素)100gg(NH4)2HPO40.25gWort(12Be')100mlGlucose(葡萄糖)10-15gCitricacid(檸檬酸)0.2gDistilledwater(蒸餾水)900mlpH5.5(左右)適用范圍:蜜環(huán)菌StarchAgar(淀粉瓊脂)Solublestarch(可溶性淀粉)10gNaNO31gMgCO31gK2HPO40.3gNaCl0.5gAgar(瓊脂)20gDistilledwater(蒸餾水)1000ml50.5BeWortAgar(5Be1^芽汁瓊脂)適用范圍:園弧青霉、少根根霉、日本根霉、爪哇根霉51.5BeKojiExtractAgar(5Be米曲汁瓊脂)適用范圍:華根霉、三寶壟根霉52.發(fā)根農(nóng)桿菌培養(yǎng)基(YEB)Beefextract(牛肉浸膏)5g/LYeastextract酵母膏)1g/LPeptone(蛋白胨)5g/LSucrose(蔗糖)5g/LMgSO4.7H2O0.4g/100mlAgar(瓊脂)1.5g/100mlpH7.4適用范圍:青枯假單胞菌53.青枯菌培養(yǎng)基(Tm)Peptone(蛋白胨)10gCasein(干酪素水解物產(chǎn))1gGlucose(葡萄糖)5gDdhro(純水)1000mlAgar(瓊脂)15gpH7.0YM(YeastMaltAgar)酵母膏3.0g麥芽汁3.0g蛋白胨5.0g瓊脂20.0g蒸餾水1000mlISP4可溶性淀粉10.0gK2HPO41.0gMgSO4.7H2O1.0gNaCl1.0g(NH4)2SO42.0gCaCO32.0gFeSO4.7H2O0.001gMnCl2.7H2O0.001g瓊脂20.0g蒸餾水1000mlTryptonSoyAgar胰蛋白胨17.0g大豆胨3.0g葡萄糖2.5g瓊脂20.0gNaCl5.0gK2HPO42.5g蒸餾水1000mlNA(NutrientAgar)牛肉膏3.0g蛋白胨5.0g瓊脂20.0g蒸餾水1000mlpH6.6-7.0甘露(糖)醇瓊脂(ATCC1)酵母膏5.0g蛋白胨3.0g甘露(糖)醇25.0g瓊脂15.0g蒸餾水1000mlMarine瓊脂2216(ATCC2)蛋白胨5.0g酵母膏1.0g檸檬酸鐵0.1g漠化鉀0.08gNaCl19.45gMgCl28.8gNa2SO43.24gCaCl21.8gKCl0.55gNaHCO30.16gNH4NO30.0016gNa2HPO40.008g氯化鍶0.034g硼酸0.022g硅酸鈉0.004g氟化鈉0.0024g瓊脂15.0g蒸餾水1000mlpH7.4-7.8兔血瓊脂(ATCC4)牛心(取其浸液)500.0g胰蛋白胨(Tryptose)10.0gNaCl5.0g瓊脂15.0g蒸餾水1000mlpH6.6-7.061.抱子形成培養(yǎng)基(ATCC5)酵母膏1.0g牛肉膏1.0g胰蛋白胨2.0g葡萄糖10.0gFeSO4微量瓊脂15.0g蒸餾水1000mlpH7.2TrypticaseSoyAgar(ATCC18)TrypticaseSoyBroth30.0g瓊脂15.0g蒸餾水1000mlYPAD(ATCC109)酵母膏10.0g蛋白胨20.0g葡萄糖20.0g瓊脂20.0g蒸餾水1000mlpH7.0SP(ATCC432)棉子糖1.0g蔗糖1.0g半乳糖1g可溶性淀粉5g水解酪蛋白2.5g瓊脂20.0gMgSO4.7H2O0.5gK2HPO40.25g蒸餾水1000mlpH自然65.胰酪胨大豆酵母浸膏瓊脂(TSA-YE)胰蛋白胨15.5g大豆蛋白胨5.0gNaCl5.0g酵母膏6.5g瓊脂15.0g蒸餾水1000mlpH7.3ATCC20蛋白胨5.0g酵母膏15.0gK2HPO43.0g葡萄糖2.0g瓊脂20.0g蒸餾水1000mlpH7.3-7.5TY(YeastTryptoneAgar)蛋白胨10.0g酵母膏5.0g葡萄糖1.0gNaCl5.0g瓊脂20.0g蒸餾水1000mlpH7.0硫醇(Thiol)培養(yǎng)基(ATCC49)示蛋白胨(ProteosePeptoneNo.3,Difco)10.0g酵母膏5.0g葡萄糖1.0gNaCl5.0g硫醇復(fù)合物8.0g瓊脂1.0gp-氨基苯甲酸0.05gpH6.9-7.3Czapek'Dox瓊脂培養(yǎng)基(ATCC134)Czapek'DoxBroth35.0g瓊脂15.0g蒸餾水1000mlMG/L蛋白胨5.0g甘露醇5.0g谷氨酸鈉1.15g生物素0.0001gK2HPO40.25gNaCl0.1gMgSO4.7H2O0.1g酵母膏2.5g瓊脂20.0g蒸餾水1000ml氯霉素(終濃度)50gg/mlpH7.0EclbBroth蛋白胨10.0g酵母膏5.0gNaCl5.0g瓊脂20.0g蒸餾水1000ml氨芐青霉素(終濃度)300gg/mlpH7.0BennettsAgar(IFO228)酵母膏1.0g牛肉膏1.0gNZAmine,typeA2.0g葡萄糖10.0g瓊脂20.0g蒸餾水1000mlpH7.3Harrold'sM40Y(ATCC319)麥芽膏20.0g酵母膏5.0g蔗糖400.0g瓊脂20.0g蒸餾水1000mlPSA(PotatoSucroseAgar)土豆200g蔗糖20g蒸餾水1000ml瓊脂20gpH6.0-6.575,培養(yǎng)基牛肉膏300g水解酪蛋白17.5g可溶性淀粉1.5g瓊脂20.0g蒸餾水1000ml磺胺嘧啶(終濃度)100gg/ml氨芐青霉素(終濃度)100gg/mlpH7.3-7.476.馬鈴薯牛肉汁培養(yǎng)基麥芽糖20.0g牛肉膏5.0g蛋白胨3.0g馬鈴薯200.0gpH7.0-7.2ISP2酵母膏4.0g麥芽汁10.0g葡萄糖4.0g瓊脂20.0g蒸餾水1000mlpH7.0BHI(BrainHeartInfusion)牛腦200.0g牛心浸出汁250.0g蛋白胨10.0g葡萄糖2.0gNaCl5.0g瓊脂20.0g蒸餾水1000mlpH6.8-7.2YPG(YeastPeptoneGlucose)酵母膏10.0g蛋白胨20.0g葡萄糖20.0g瓊脂20.0g蒸餾水1000mlpH7.080.兔食瓊脂培養(yǎng)基(ATCC340)兔食(商店出售)25.0g瓊脂20.0g蒸餾水1000ml*煮沸兔食,并浸泡1/2小時(shí),用多層紗布過濾,加瓊脂于濾液中。81.土豆瓊脂(ATCC423)200克去皮土豆切成小塊于1000ml自來水中煮沸1小時(shí),用細(xì)棉布過濾,加進(jìn)5.0mgMnSO4,pH調(diào)至6.8,用自來水定容至1000ml。加進(jìn)15.0g瓊脂,溶化,分裝并滅菌。82,土豆浸汁加無機(jī)鹽培養(yǎng)基(ATCC470)土豆2000gMnSO4.5H2O0.02gMgSO4.7H2O0.4gZnSO4.7H2O0.01gCuSO4.5H2O0.01gFeSO4.7H2O0.1gCaCl2.2H2O0.1gK2HPO40.5g*稱取200g去皮塊狀土豆,加入1000ml蒸餾水煮沸30分鐘,多層紗布過濾,濾液中加入以上成分,溶解后定容至1000ml。83.無機(jī)鹽淀粉瓊脂培養(yǎng)基(ATCC527)可溶性淀粉10.0gK2HPO41.0gMgSO41.0gNaCl1.0g(NH4)2SO42.0gCaCO32.0gFeSO4.7H2O0.001gMnCl2.7H2O0.001gZnSO4.7H2O0.001g瓊脂20.0gpH7.0-7.484.(AS125)新鮮雞蛋15-16蒸餾水375ml2%孔雀(石)綠10.0gK4HPO41.5gMgSO4.7H2O0.375g天冬酰胺2.25g甘油7.5ml土豆粉18.8g85.燕麥片(Oatmeal)瓊脂培養(yǎng)基燕麥片60.0g瓊脂12.5g蒸餾水1000ml*將燕麥片加600ml水中,制成勻漿,加熱至45-50OC,然后加入已溶化瓊脂的另400ml水,121oC滅菌90分鐘。86.細(xì)菌培養(yǎng)基(ATCC573)(NH4)2SO41.3gK2HPO40.37gMgSO4.7H2O0.25gCaCl2.2H2O0.07gFeCl30.02g葡萄糖1.0g酵母膏1.0g蒸餾水1000ml用10NH2SO4調(diào)pH至4.0。若配制固體培養(yǎng)基,為增強(qiáng)上述溶液的強(qiáng)度,pH需調(diào)至3.5,瓊脂水溶液濃度為40g/L。兩者分別高壓滅菌,冷卻至約50oC,無菌操作將兩種溶液等體積混合,這一過程可避免瓊脂被酸水解。87,碎肉培養(yǎng)基(ATCC593)牛肉(去脂肪)500.0g蒸餾水1000mlNaOH25.0ml*所用瘦牛肉或馬肉(碾)磨碎前需去脂肪和周圍的組織。將肉、水、NaOH混合后邊攪拌邊加熱,冷至室溫,去掉表面油脂,過濾,留下肉末和濾液。濾液加蒸餾水至1000ml。濾液中加下列成分:蛋白胨30.0g酵母膏5.0gK2HPO45.0g0.025%刃天青(Resazurin)溶液4.0ml然后煮沸,冷卻,加0.5gL-半胱氨酸HCl。H2O,pH調(diào)至7.0。在氧氣、氮?dú)猓∣2-freenitrogen)和3%氫氣條件下,按1份碎肉加4-5份以上溶液的比例分裝于每支試管。在氮?dú)夂蜌錃鈼l件下,使用橡皮塞,快排氣,高壓滅菌15分鐘。YMPG酵母膏3.0g麥芽汁3.0g蛋白胨5.0g葡萄糖10.0gFeSO4.7H2O0.1g瓊脂20.0g蒸餾水1000mlpH6.8滅菌:110oC,35分鐘TGYM培養(yǎng)基(ATCC679)胰蛋白胨5.0g葡萄糖1.0g酵母膏3.0g蒸餾水1000mlDL-蛋氨酸(DL-Meth0.5gTYG培養(yǎng)基(ATCC741)胰蛋白胨3.0g酵母膏3.0g葡萄糖3.0gK2HPO41.0g瓊脂20.0g蒸餾水1000mlpH7.4M9Na2HPO412.8gKH2PO43.0gNaCl0.5gNH4Cl0.5g葡萄糖(單獨(dú)滅菌)10.0g維生素B1(單獨(dú)滅菌)1〃g/mlpH自然ISP3(IFO245)燕麥粉20.0g微量元素溶液1mlpH7.2微量元素:FeSO4.7H2O0.1gMnCl2.4H2O0.1gZnSO4.7H2O0.1gOatmealAgar瓊脂5.0g蒸餾水500ml加熱溶化速溶燕麥粉40.0g蒸餾水250ml加熱溶化后與瓊脂液混合加水至1000mlpH5.8V8-JuiceAgar(AYCC343)V-8果汁200gCaCO33.0g瓊脂20.0g蒸餾水1000mlpH5.8Czapek-DoxAgar(ATCC312)NaNO33.0gK2HPO41.0gMgSO4.7H2O0.5gKCl0.5gFeSO4.7H2O0.01g蔗糖(單獨(dú)滅菌)30.0g瓊脂20.0g蒸餾水1000mlpH5.6GC培養(yǎng)基(ATCC814)A液瓊脂5.0gGC瓊脂培養(yǎng)基(BBL11275)36.0g蒸餾水500.0mlB液干燥色牛血紅蛋白(BBL11871)10.0g蒸餾水500.0mlIsoVitaleX(BBL11876)10.0ml固氮螺菌培養(yǎng)基(ATCC838)K2HPO40.1gKH2PO40.4gMgSO4.7H2O0.2gNaCl0.1gCaCl20.02gFeCl30.01gNaMoO4.2H2O0.002g蘋果酸鈉(Namalate)5.0g酵母膏0.05g蒸餾水1000mlpH7.2-7.4BG-11NaNO31.5gK2HPO40.04gMgSO4.7H2O0.075gCaCl2.7H2O0.036gNa2CO30.02g檸檬酸0.006g檸檬酸鐵0.006g*微量元素溶液A51ml氨芐青霉素(終濃度)50Mg/m蒸餾水1000ml瓊脂20.0g*微量元素溶液A5H3BO42.86gMnCl2.4H2O1.81gZnSO40.222gNa2MoO40.39gCuSO4.5H2O0.079gCo(NO3)2.6H2O49.4g(MarineAgar)蛋白胨5.0g酵母膏1.0g檸檬酸鐵0.1gNaCl19.45gMgSO4.7H2O5.9gKCl0.55gNa2SO43.24gCaCl21.8gNaHCO30.16gKI0.08g微量元素10mlpH7.0-7.6*微量元素溶液:氯化鍶0.034g硅酸鈉0.004g蒸餾水1000mlH3BO40.022gNaCl0.0024gNaNO30.0016gNaHPO40.008g蒸餾水1000ml(YeastStarchAgar)酵母膏2.0g可溶性淀粉10.0g瓊脂20.0g蒸餾水1000mlpH7.2-7.4GYMC葡萄糖4.0g酵母膏2.0g麥芽汁20.0g瓊脂20.0gCaCO31.5g蒸餾水1000mlpH6.8-7.0LCSB乳糖15.0g玉米漿5.0g蛋白胨5.0gNaCl4.0gMgSO4.7H2O0.5gKH2PO40.6gFeCl3.6H2O0.005gCuSO4.5H2O0.002g瓊脂20.0g蒸餾水1000ml相對濕度65%pH4.8.高氏1號(G)可溶性淀粉20.0gKNO31.0gK2HPO40.5gMgSO4.7H2O0.5gNaCl0.5gFeSO4.7H2O0.01g瓊脂20.0g蒸餾水1000mlpH7.0-7.2.葡萄糖-酵母膏瓊脂(ATCC846)酵母膏5.0g蛋白胨1.0gKH2SO41.0gNaCl1.0g瓊脂20.0g蒸餾水950.0mlpH6.8補(bǔ)加50ml10%葡萄糖(葡萄糖溶液單獨(dú)過濾滅菌)。Tep尿液(UricAcid)瓊脂(ATCC894)Na2HPO4.12H2O9.0gKH2PO41.5gMgSO4.7H2O0.2g檸檬酸鐵銨(綠色)1.2gCaCl220.0mgMnCl2.4H2O1.0mgTrypticcaseSoyBroth(BBL11768)3.0g尿素4.0g瓊脂20.0蒸餾水1000mlpH7.2增強(qiáng)梭菌生長的培養(yǎng)基(ATCC1053)胰蛋白胨10.0g牛肉膏10.0g酵母膏3.0g葡萄糖5.0gNaCl5.0g可溶性淀粉1.0g半胱氨酸鹽酸鹽0.5g醋酸鈉3.0g瓊脂15.0g蒸餾水1000mlpH6.6-7.0酵母膏-甘露(糖)醇瓊脂(ATCC1205)K2HPO40.5gMgSO4.7H2O0.2gNaCl0.1g甘露(糖)醇10.0g酵母膏0.4g蒸餾水加至1000mlTomatoDextrinAgar(ATCC965)番茄汁20.0g糊精20.0g酵母膏10.0gCoCl2.6H2O5.0mg瓊脂20.0g蒸餾水1000mlpH7.2-7.4嗜熱菌無機(jī)鹽溶液(ATCC1554)FeSO41.0mgMgSO4.7H2O200.0mgNa2HPO4210.0mgNaH2PO490.0mgKCl40.0mgCaCl215.0mgNaNO30.25gNH4Cl0.25g微量元素溶液(見下面)10.0mln-Heptadecane(C17)0.1%(v/v)蒸餾水1000ml微量元素溶液:CuSO4.5H2O500mcgH3BO41.0mgMnSO4.5H2O7.0mcgZnSO4.7H2O7.0mgMoO31.0mgCoSO4.7H2O18.0mcg蒸餾水1000ml110,醋桿菌培養(yǎng)基(AS1)葡萄糖100.0g酵母膏10.0gCaCO320.0g瓊脂15.0g蒸餾水1000mlpH6.8111.葡萄糖天冬酰胺瓊脂培養(yǎng)基(AS11)葡萄糖10.0g天冬酰胺(Asparagine)0.5gK2HPO40.5g蒸餾水1000mlpH7.2-7.4麥芽汁瓊脂(AS13)稀釋去除啤酒花的麥芽汁至濃度為12Brix(白利-糖濃度單位)。按1.5%的比例將瓊脂加進(jìn)上述麥芽汁中,加熱溶解,然后將此培養(yǎng)基分裝試管中。高壓滅菌:110C30分鐘。(ATCC805)酵母膏1.0gK2HPO40.7gMgSO4.7H2O1.0g甘露糖醇5.0gKH2PO40.1g蒸餾水1000ml瓊脂20.0gpH7.4Todd-HewittMedium牛心臟500.0g蛋白胨20.0g葡萄糖2.0gNaCl2.0gNa2HPO40.4gNa2CO32.5g瓊脂20.0g蒸餾水1000mlpH7.8MSK酵母膏1.0g脫脂奶粉100.0g瓊脂20.0g蒸餾水1000mlpH6.9116.土豆葡萄糖瓊脂培養(yǎng)基(AS14)土豆500g葡萄糖20.0g瓊脂20.0g*稱取500克土豆,去皮切成丁,立即加A1000ml水,充分煮沸,用棉布過濾。濾液用水定容至1000ml,再加入其它成分。高壓滅菌:115C20分鐘。PCA(ATCC343)土豆20.0g胡蘿卜20.0g瓊脂20.0g蒸餾水1000mlpH5.8(Mueller-HintonAgar)牛肉膏300.0g水解酪蛋白17.5g淀粉1.5g磺胺嘧啶(終濃度)50gg/ml瓊脂20.0g蒸餾水1000ml氨芐青霉素(終濃度)50gg/mlpH7.2119,豆餅粗粉瓊脂培養(yǎng)基(AS20)10%豆餅粗粉浸液1000g葡萄糖10.0gNaCl2.5g瓊脂15.0g120.北京棒狀桿菌噬菌體培養(yǎng)基(AS48)(1)牛肉膏3.0g蛋白胨5.0gNaCl5.0g葡萄糖10.0g蒸餾水1000mlpH7.0(2)葡萄糖20.0g玉米漿8.0gK3PO4.3H2O1.0gMgSO4.7H2O0.4g尿素(Urea)5.0gFe+,Mn+分別為2ppm蒸餾水1000mlpH7.0121.營養(yǎng)肉湯和葡萄糖培養(yǎng)基(AS100)牛肉膏10.0g蛋白胨10.0g葡萄糖10.0gNaCl5.0g瓊脂15.0-20.0g蒸餾水1000mlpH7.0(TrypticaseSoyaAgar)胰蛋白胨17.0g大豆胨3.0g葡萄糖2.5gNaCl5.0gK2HPO42.5g甘油(50%)瓊脂20.0g蒸餾水1000mlpH7.0尿囊素?zé)o機(jī)鹽培養(yǎng)基(DSM6)K2HPO40.8gKH2PO40.2gMgSO4.7H2O0.5gCaCl2.2H2O0.05gFeSO4.7H2O0.01gMnSO4.H2O1.0mg尿囊素(Allantion)20.0g瓊脂15.0g蒸餾水1000ml124,堿性營養(yǎng)瓊脂培養(yǎng)基(DSM31)蛋白胨5.0g牛肉膏3.0g瓊脂20.0g蒸餾水1000ml滅菌后,加入無菌的1MNa-Sesquicar-bonate溶液:(1:10v/v),至pH9.7。Na-Sesquicarborbonate溶液:NaHCO34.2gNa2CO35.3g蒸餾水100.0mlTrypticaseSoy瓊脂培養(yǎng)基(DSM220)酪蛋白胨(Peptonefromcasein)5.0g豆粉蛋白胨(Peptonefromsoymeal)5.0gNaCl5.0瓊脂15.0g蒸餾水1000mlpH7.3(I

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