測(cè)序通用引物

載體名稱上游引物（5'primer）下游引物（3'primer）P3*FLAG-CMVCMV-30(825-854)CMV-24(1129-1152)pAc5.1/V5-His(A,B,C)pAc5-5BGHrevpAcG2TpGEX-5notavailablepACTT7EEVT3/EBVrevpACT2GAL4ADforpGAL4-ADrvpACT2p17110p12584pACYC184(BamHI-site)PBRforBamPBRrevBampACYCDuet-1(MCSI)ACYCDuetUP1PrimerDuetDOWN1pACYCDuet-1(MCSII)DuetUp2T7-TerminatorpAdEasy-1notavailablenotavailablepAdTrack-CMVnotavailablenotavailablepAS2-1GAL4Bdfor/P24990pGAL4-BDrv/P31430pB42ADpB42ADFPB42ADRpBABEpBMN5'pBacPAK8BAC1BAC2pBADpBAD-FpBAD-RpBAD/HisABCpBAD-5'pBBR1MCST3/M13revT7/M13forpBD-GAL4pBD-GAL4-FPBD-GAL4-RpBI12135SNOSpBINDGAL4-BdforT3,EBVrevpBK-CMVM13F/T7M13R/T3pBluescriptIIKS(+/-)T3/M13revT7/M13for(優(yōu)先用M13F)pBluescriptIISK(+/-)T3/M13revT7/M13forpBluescriptKS(+/-)T3/M13revT7/M13forpBluescriptSK(+/-)T3/M13revT7/M13forpBR322(BamHI-site)PBRforBamPBRrevBampBR322(EcoRI/HindIII-sites)pBRforEcopBRrevHindpBR325(BamHI-site)PBRforBamPBRrevBampBR329(BamHI-site)PBRforBamPBRrevBampBridge(MCSI)GAL4-Bdfor3'BDpBridge(MCSII)notavailablenotavailablepBS-TIIT3/M13revT7/M13forpBS(AMP)T7T3pBSRT3T7terpBudCE4.1(MCSI)CMV-Profor,T7c-mycrev,EBVrevpBudCE4.1(MCSII)EF-laFwdBGHpBudCE4.1/lacZ/CATCMV-Profor,T7c-mycrev,EBVrevpBV220pBV220FPBV220RpCAL-cT7/Tac-ProforT7-Terminator

pCAMBIA1301(1300)pCAMBIA1301-5'(ECORI)/M13RpCAMBIA1301-3'(HindIII)pCAMBIA1304pCAMBIA1301-5'(ECORI)pCAMBIA1301-3'(HindIII)pCAMBIA2300M13R(-48)M13F(-47)/M13F(-49)pCANTB5ES1S6pCAT3-enhancerRVP3此載體無反向引物pCDFDuet-1(MCSI)ACYCDuetUP1PrimerDuetDOWN1pCDFDuet-1(MCSII)DuetUP2T7TERpcDNA1.1CMV-Profor,T7pCDM8-3pcDNA2.1M13for/T7M13rev/Tac-ProforpcDNA3T7/CMV-ProforBGHrev/SP6pcDNA3.1(+/-)pcDNA3.1F/T7/CMV-ProforBGHrevpcDNA3.1/His(A,B,C)T7/CMV-ProforBGHrevpcDNA3.1/myc-His(A,B,C)T7/CMV-ProforBGHrev/c-mycrevpcDNA3.1/V5-His(A,B,C)T7/CMV-ProforBGHrev/V5revpcDNA3.1/Hygro(+,-)T7/CMV-ProforBGHrev/V5revpcDNA3.1/Zeo(+.-)T7BGHrevpcDNA3.3TOPOTACMV-ProforTKPAreversepcDNA4/HisMax(A,B,C)XpressforBGHrevpcDNA4/HisMax-T0P0XpressforBGHrevpcDNA4/HisMax-T0P0/lacZXpressforBGHrevpcDNA4/myc-His(A,B,C)T7,CMV-ProforBGHrev,c-mycrevpcDNA4/TOTREfor/CMV-ProforBGHrevpcDNA4/V5-His(A,B,C)CMV-Profor,T7BGHrev,V5revpcDNA4-TET/ON/AMP+CMV-ProforpcDNA5/FRTT7/CMV-ProforBGHrevpcDNA6/myc-His(A,B,C)CMV-Profor,T7BGHrev,c-mycrevpcDNA6/V5-His(A,B,C)CMV-Profor,T7BGHrev,V5revpCEP4pCEP-F/CMV-ProforEBVrevpCIT7(17BASE)EBVrevpCI-neoT7(17BASE)EBVrev,T3pCMS-EGFPT7T3pCMS-EGFPT7EBVrev,T3pCMV-3Tag-4AT3/PFLAG-CMV-FT7pCMV5pCMV5FPCMV5RpCMV6CMV-ProforpCMV6-3、HGHrevpCMV6-XL4CMV-Profor/T7pCMV6-3、HGHrevpCMV-HAPCMV-F,CMV-Profor,TREforPCMV-R,EBVrevpCMV-MYC(AMP+)PCMV-FPCMV-R,EBVrevpCMVmyc/nucCMV-ProforBGHrev,c-mycrevpcmv-scriptEXVectorT3T7pCMV-Sport6T7/M13forSP6/M13revpCOLADuet-1(MCSI)ACYCDuetUP1PrimerDuetDOWN1pCAL-nT7/Tac-ProforT7-Terminator

pCR2.1T7,M13forM13revpCR2.1-TOPOM13for,T7M13revpCR3.1T7BGHrevpCR3.1-UniT7BGHrevpCR4T3/M13revT7/M13forpCR4-BluntT3/M13revT7/M13forpCR4-TOPOM13rev,T3M13for,T7pCR-BluntM13revT7,M13forpCR-BluntllM13rev/SP6T7/M13forpCR-Bluntll-TOPOM13rev/SP6M13for/T7pCRIIM13rev/SP6T7/M13forpCRII-TOPOM13rev/SP6M13for/T7pCR-ScriptSK(+)T7/M13forT3/M13revpCR-XL-TOPOM13revT7,M13forpCS2+(MCSI)SP6EBVrev,T7?pCS2+(MCSII)T3pSG5-3pDBGAL4camT7PpDBleuPDBLeu-5PDBLeu-3pDEST22DEST22FDEST22RpDEST15pGEX-5T7-TerminatorpDEST20pGEX-5pFastBac-3pDEST26TREfor/CMV-ProforT7/M13forpDisplayT7/CMV-Proforc-mycrevpDNR-LIB(MCSA)M13for,T7pSG5-3,M13RpDonarM13FM13RpDONR201PDONR-FPDONR-RpDONR207PDONR-FPDONR-RpDONR223M13FM13RpDriveT7/M13revSP6/M13forpDsRED1-C1(DsRed1-N-f)DsRed1-C-rpDsRed1-N1CMV-ProforRFP-Nrev,CMV-R?pDsRed2-N1(DsRed1-N-f)DsRed1-C-rpDsRed2-C1(DsRed1-N-f)DsRed1-C-rpDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3'pDsRed-ExpressdsRed1Nprimer/CMV-FdsRed1Cprimer/CMV-RpDSRED-monomer-N1(KAN+)dsRed1NprimerpDSRED-N1(KAN+)pEGFP-N-5'pDSRED-N-RpEASY-BluntM13FT7/M13RpEASY-BluntSimpleM13FT7/M13R（距離插入位點(diǎn)合適，優(yōu)先安排）pEASY-T1SimpleM13FM13R（距離插入位點(diǎn)合適，優(yōu)先安排）pCOLADuet-l(MCSII)DuetUP2T7TER

pEASY-TlM13F/T7M13RpEASY-T3M13F/T7M13R/SP6pECFP-N(C)PECFP-N-5.PECFP-N-3,pEF/myc/cyto/ER/mito/nucpEF-5Pcdna3.1R/BGHrevpEF1(4,6/myc-His)T7/pEF-5Pcdna3.1R/BGHrevpEF1(4,6/V5-His)T7/pEF-5Pcdna3.1R/BGHrevpEF1(4;6/HisA;B;C)T7/XpressforPcdna3.1R/BGHrevpEF5/FRT/V5-D(-TOPO)T7/pEF-5Pcdna3.1R/BGHrevpEGFPM13revEGFP-NrevpEGFP-1notavailableEGFP-Nrev/PEGFP-N-3”pEGFP-CPEGFP-C-5'PEGFP-C-3'pEGFP-C1EGFP-CforSV40-pArevpEGFP-C2EGFP-CforSV40-pArevpEGFP-C3EGFP-CforSV40-pArevpEGFP-FEGFP-CforSV40-pArevpEGFP-NPEGFP-N-5'PEGFP-N-3”pEGFP-N1CMV-ProforEGFP-NrevpEGFP-N2CMV-ProforEGFP-NrevpEGFP-N3CMV-ProforEGFP-NrevpENTRM13FM13RpET-11(-a,-b,-c,-d)T7T7-TerminatorpET-12(-a,-b,-c)T7T7-TerminatorpET-14bT7T7-TerminatorpET-15bT7T7-TerminatorpET-16bT7T7-TerminatorpET-17bT7T7-TerminatorpET-17xbT7T7-TerminatorpET-19bT7T7-TerminatorpET-20b(+)T7T7-TerminatorpET-21(-a,-b,-c,-d)(+)T7T7-TerminatorpET-22b(+)T7T7-TerminatorpET-23(-a,-b,-c,-d)(+)T7T7-TerminatorpET-24(-a,-b,-c,-d)(+)T7T7-TerminatorpET-25b(+)T7T7-TerminatorpET-26b(+)T7T7-TerminatorpET-27b(+)T7T7-TerminatorpET-28a(-b,-c)(+)T7T7-TerminatorpET-29a(-b,-c)(+)S.tag/T7T7-TerminatorpET-3(-a,-b,-c,-d)T7T7-TerminatorpET-30Ek/LICT7T7-TerminatorpET-30Xa/LICT7T7-TerminatorpET-30a(-b,-c)(+)S.tag/T7T7-TerminatorpET-31b(+)notavailableT7-Terminator

pET-32XA/LICTRXforT7-TerminatorpET-32a(-b,-c)(+)S.tag/TRXforT7-TerminatorpET-33b(+)T7T7-TerminatorpET-34b(+)S.tagT7-TerminatorpET-35b(+)S.tagT7-TerminatorpET-36b(+)S.tagT7-TerminatorpET-37b(+)S.tagT7-TerminatorpET-38b(+)notavailableT7-TerminatorpET-39b(+)S.tagT7-TerminatorpET-40b(+)S.tagT7-TerminatorpET-41Ek/LICGST-CforT7-TerminatorpET-41a(-b,-c)(+)S.tag/pGEX-5T7-TerminatorpET-42a(-b,-c)(+)S.tag/pGEX-5T7-TerminatorpET-43.1a(-b,-c)(+)S.tagcoliDOWNpET-43.1aEK/LICS.tagnotavailablepET-44a(-b,-c)(+)S.tagT7-TerminatorpET-44aEK/LICS.tagnotavailablepET-45b(+)T7T7-TerminatorpET-46Ek/LICT7T7-TerminatorpET-5(-a,-b,-c)T7T7-TerminatorpET-52b(+)T7T7-TerminatorpET-7T7T7-TerminatorpET-9(-a,-b,-c,-d)T7T7-TerminatorpETcoco-1T7T7-TerminatorpETDuet-1(MCSI)pETUP1DuetDOWN1pETDuet-1(MCSII)DuetUp2T7-TerminatorpET-T4T7T7-TerminatorpEYFP-C1pEGFP-C-5'pEGFP-C-3'pEYFP-N1pEGFP-N-5',CMV-ProforpEGFP-N-3'pFLAG-ATSN26forpTrcHis-rv；C24revpFLAG-CTCN26forpTrcHis-rv；C24revpFLAG1N26forpTrcHis-rv；C24revpFLAG-CMV(-2)CMV-30；CMV-ProforCMV-24pFLAG-CMV1CMV-30CMV-24pGAD424GAL4ADfor3'ADpGADGHGAL4ADfor3'ADpGADT7T7/GAL4ADfor3'ADpGADT7-RecT7/GAL4ADfor3'ADpGADT-TT73,BDpGAD10GAL4ADfor3'ADpGAPZa-Aa-FACTOR3'AOXpGBKT7T73,BDpET-32Ek/LICTRXforT7-Terminator

MATCHMAKER5'DNA-BD/GALpGBT94BD MATCHMAKER3'DNA-BDpGEM-1T7SP6pGEM-3T7SP6pGEM-3Zf(+)(-)SP6/M13revT7/M13forpGEM-4T7SP6pGEM-4ZT7/M13forSP6/M13revpGEM-5Zf(+)(-)SP6/M13revT7/M13forpGEM-7Zf(+)(-)SP6/M13revT7/M13forpGEM-9Zf(+)(-)T7/M13forSP6/M13revpGEM-TT7/M13forSP6/M13revpGEM-TEasyT7/M13forSP6/M13revpGene/V5-HisA(B,C)pGENE-5BGHrev,V5revpGEX-2TpGEX-5pGEX-3pGEX-2TKpGEX-5pGEX-3pGEX-3XpGEX-5pGEX-3pGEX-4T-1(-2,-3)pGEX-5pGEX-3pGEX-5X-1(-2,-3)pGEX-5pGEX-3pGEX-6P-l(-2,-3)pGEX-5pGEX-3pGL2GLP1GLP2pGL3RVP3GLP2（RVP4）（沒有特別說明用GLP2）pGL3-Basic(MCSI)RVP3(PGL3+)?GLP2(PGL3-)?pGL3-Basic(MCSII)RVP4,PLXSN-3'?pGL4pGlow(-TOPO)T7pGlow-3pGM-TT7SP6pGWCSP6T7pHook-2T7/CMV-ProfornotavailablepIND/V5-HisA(B,C)notavailableBGHrev,V5revPinpointTMVectorPinpointPrimerSP6pIRES(MCSI)T7pIRES-3pIRES(MCSII)notavailableT3,EBVrevpIRES2-EGFPCMV-Profor,pIRES2-EGFP.P5'IRESrev,pIRES2-EGFP.P3'pIRES-hrGFP-2aCMV-Profor,T3pIRES-hrGFP-3pIRESneo2T7/CMV-ProforpIRES-3pIVEX(all)T7/pBRrevBamT7-TerminatorpLEXApLEXA-FpLEXA-RpLNCXpLNCX-FpLNCX-RpLNCX2CMV-ProforpRevTRE-3pLVTHM（H1啟動(dòng)子下游）H1primerSP6pLXSNpLXSN-FpLXSN-RpMALcMalEprimerM13F(-47)

pMAL-c2EMalEprimerM13F(-47)pMAL-C2xPmal-c2x-F(P5')Pmal-C2X-R(P3')pMALdMalEprimerM13F(-47)pMALeMalEprimerM13F(-47)pMAL-P4XMalEprimerM13F(-47)pMAL-p2XMalEprimerM13F(-47)pMETA/B/CAUG1FAUG1RpMD18-TM13R(-48)ECORIM13F(-47)(HINDIII)pMD19-TM13F47M13R48pMD20-TM13F47M13R48pMOSBlueT7/M13revM13forpMSCV-puropLXSNfwpMSCVrevpMT/V5-HisA(B,C)MtforBGHrev,V5revpNTAPT3T7pCTAPpCTAP5'primerT7pOTB7M13rev/T7M13for/SP6pOptiVEC-TOPOTACMV-F/CMV-ProforEMCVIRESreversepPC86PPC86-FPPC86-RpPC97PBD-GAL4-FPBD-GAL4-RpPIC3.5K5'AOX3'AOXpPic9k(A+,Z+)5'AOX(Ppic9k-f)/a-factor3'AOX(Ppic9k-R)pPICZa5'AOX/PPICZa-F3'AOXpPROEXHTAM13R(-48)無反向引物pProEX-HTa(b,c)M13R48/Tac-ProforpBADrv/pTrcHis-3pPD129.36M13F20/M13FpQE30or40or60(AMP+)PQE30-FPQE-30RpRC/CMV2/CATCMV/T7pRECEIVERCMV-F此載體無反向引物pRECEIVER-M03pRECEIVER-M03FpRECEIVER-M03RpREP4pREP4forwardprimerEBVreverseprimerpRK5SP6pSG5-3pRSETT7/XpressforT7terpRSFDuet-1(MCSI)DuetUP1DuetDOWN1pRSFDuet-1(MCSII)DuetUP2T7TERpSecTac2T7/CMV-ProforBGHrev/c-mycrevpSG5pSG5-5,/T7PSG5-R(SV40)pSHlox1T7SP6pSHUTTLE-CMVPSHUTTLE-CMV-FPSHUTTLE-CMV-RpSIT7EBVrevpSilencer1.0-U6T7T3pSilencer2.0-U6T7M13forpSilencer2.1-U6neoT7M13forpSilencer3.1-H1hygroM13F(-47)3.0REV

pSILENCEV2.0-U6T72.0REVpSIneoT7EBVrev,T3PsitT7T7TpSK01-TM13FM13RpSK-CMV(KAN+)T7T3pSOSPsos5'Psos3'pSP64SP6M13revpSP64/65SP6此載體無反向引物pSP65SP6M13revpSP70SP6T7pSP71SP6T7pSP72SP6T7pSP73SP6T7pSport1SP6/M13F(-47)T7/M13RpSPORT1SP6/M13forT7/M13revpSPT18SP6T7pSTBlueT7/M13revSP6/M13forpSUPER.BasicT7/M13FM13RpSUPERT7T3pSURE-TM13FM13RpSURE-TsimpleM13F-47M13R-48pSuperCospBRforEcoT7pT3T7M13rev/T3M13for/T7pT7-7T7notavailablepT7Blue?T7M13F(-47)pT7T318UM13rev/T7M13for/T3pT7T3D-PACM13rev/T7M13for/T3pTA2M13F/T7(優(yōu)先使用M13F)T3/M13RpT-AdvM13revT7,M13forpTAgT7/M13revM13forpTARGETTMpTarget.F(在T7前面)/T7pTarget.RpTG19-TM13F(-47)M13R(-48)pTARGETTMT7/M13FPTARGET-SEQpThioHisA,B,andCTrxForwardTrxReverse/M13forpTO-T7T7T7TERpTRC99APTRC99A-FpTrcHis-rvpTrc99a-cPTRC99C-FPTRC99C-RpTRC99a-cPTRC99C-FPTRC99C-RpTrcHisBPTrcHisA-F/XpressforPTrcHisA-RpTrcHisAPTrcHisA-FPTrcHisA-RpT-REX-DEST30TREforT7pTriEx1T7/TriExupTriExdownpSilencer4l-CMVneopSilener4.1-5'CMV-Profor

pTriExl.lT7/TriExupTriExdownpTriEx1.1HygroT7/TriExupnotavailablepTriEx1.1NeoT7/TriExupIRESrevpTriplEXlTriplEx-LD-5T7,TriplEx-LD-3pTriplEX2TriplEx-LD-5；pTR5‘T7,TriplEx-LD-3pTWIN-1T7T7TERpTXB1T7MxeInteinrevpTXB2T7MxeInteinrevpTXB3T7MxeInteinrevpTYB1T7InteinrevpTYB2T7InteinrevpTYB3