血涂片制備染色及白細(xì)胞形態(tài)觀察【-】課件

血涂片制備染色及白細(xì)胞形態(tài)觀察臨床檢驗(yàn)基礎(chǔ)與輸血教研室血涂片制備染色及白細(xì)胞形態(tài)觀察臨床檢驗(yàn)基礎(chǔ)與輸血教研室1根據(jù)不同的細(xì)胞種類及細(xì)胞的不同成分，對(duì)酸性及堿性染料的結(jié)合能力不同，而使各種細(xì)胞呈現(xiàn)出各自的特點(diǎn)。掌握血涂片的制備和染色方法及白細(xì)胞的正常形態(tài)原理

目的根據(jù)不同的細(xì)胞種類及細(xì)胞的不同成分，對(duì)酸性及堿性染料的結(jié)合能2器材載玻片、推片、洗耳球、顯微鏡、微量吸管、香柏油、乙醇-乙醚溶液、拭鏡紙?jiān)噭┤鹗先疽?、磷酸緩沖液標(biāo)本抗凝血器材載玻片、推片、洗耳球、顯微鏡、微量吸管、香柏油、乙醇3血涂片制備推片載玻片血液將推片勻速向前，勿停頓。涂片的厚薄取決于速度和角度：快、大(厚)。慢、?。ū。┮粡垵M意的血涂片應(yīng)厚薄均勻頭體尾鮮明涂片干燥后用鉛筆在血涂片的頭部寫上姓名和編號(hào)李四B123血涂片制備推片載玻片血液將推片勻速向前，勿停頓。涂片的4血涂片染色勿沖洗再加瑞氏染色Ⅱ液5-8滴后將Ⅰ液和Ⅱ液充分混勻靜置10分鐘直接流水沖洗3-5分鐘（切勿先倒掉染液）涂片經(jīng)水洗干燥后用油鏡分類計(jì)數(shù)100個(gè)白細(xì)胞李四123涂片干燥后加瑞氏染色Ⅰ液5-8滴覆蓋整個(gè)血膜1分鐘血涂片染色勿沖洗再加瑞氏染色Ⅱ液5-8滴后將Ⅰ液和Ⅱ液5血涂片的觀察低倍鏡觀察1.染色情況(滿意、偏酸、偏堿）2.細(xì)胞分布情況油鏡下分類計(jì)數(shù)：體尾交界處。油鏡分類時(shí)應(yīng)按一定走向，不要重復(fù)計(jì)數(shù)。李四123血涂片的觀察低倍鏡觀察李四1236中性桿狀核粒細(xì)胞中性桿狀核粒細(xì)胞7

中性分葉核粒細(xì)胞中性分葉核粒細(xì)胞8

嗜酸性粒細(xì)胞嗜酸性粒細(xì)胞9嗜堿性粒細(xì)胞嗜堿性粒細(xì)胞10

單核細(xì)胞單核細(xì)胞111.本次實(shí)驗(yàn)的關(guān)鍵環(huán)節(jié)是什么？

2.每人分類的結(jié)果與本組其他人使用同一標(biāo)本的同學(xué)是否一致，分析原因。分析討論分析討論12K+oN3sR6vVazYdC$hG-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7wWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6vVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8xXcB#fF&jI)mM1qP4tT8AZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT8yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS7xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR6wVazYeD$hG*lK+oN2sR6vU9zYdC!hG*kJ-oN2rQ5vU9yXdC!gF&kJ-nM1rQ5uT8yXcB#gF&jI)nM1qP4uT8A#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jI)mL1qP4tS8xWbA#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jI)mL1qP4tS8xWbA#fE%iH)mL0pO4tS7wWbAZeD%iH(lK0pO3sR7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rQ6vU9yYdC!gF*kJ-nM2rQ5uT9yXcB!gF&jI-nM1qP5uT8xXcB#fE&jL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2rR6vU9zYdC!gG*kJ-nN2rQ5uU9yXcC!gF&jJ-nM1qQ5uT8yXcB#fF&jI)mM1qP4tT8xWbB#fE%iI)mL0pP4tS7xWbAZeE%iH(lL0pO3sS7wVaAZeD$hH(lK+oO3sR6wVazYdG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbAZeD$iH(lK+pO3sR7wVazYeD$hG*lK+oN2sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM1rQ5uT9yXcB#gF&jI)nM1qP4uT8xWcB#fE%jI)mL0qP4tS8xWbAZfE%iH(mL0pO3tS7wVbA$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rR6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qQ5uT8xXcB#fE&jI)mL1qP4tT8xWbA#fE%iH)mL0pP4tS7wWbAZeD%iH(lK0pO3sS7wVazZeD$hG(lK+oN3sR6vVazYdC$hG*kJ+oN2rR6vU9yYdC!gF*kJ-nM2rQ5uU9yXcB!gF&jI-nM1qQ5uT8xXcB#fE&jI)mL4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+pO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-oN2rQ5vU9yXcC!gF&jJ-nM1rQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0qP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+pO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-nN2rQ5vU9yXcC!gF&jJ-nM1rQ5uT8yXcB#fF&jI)mM1qP4uT8xWbB#fE%iI)mL0qP4tS7xWbAZeE%iH(lL0pO3tS7wVaAZeD$hH(lK+oO3sR6wVazYdD$hG*kK+oN2sR6vU9zYdC!gG*kJ-nN2rQ5vU9y#gF&jI-nM1qP5uT8xWcB#fE%jI)mL1qP4tS8xWbAZfE%iH(mL0pO4tS7wVbAZeD$iH(lK0pO3sR7wVazYeD$hG*lK+oN3sR6vUazYdC!hG*kJ+oN2rQ6vU9yXdC!gF&kJ-nM2rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB#fE%jI)mL1qP4tS8xWbAZfE%iH(mL0pO4tS7wVbAZeD$iH(lK0pO3sR7wVazYeD$hG*lK+oN3sR6vUazYdC!hG*kJ-oN2rQ6vU9yXdC!gF&kJ-nM2rQ5uT9yXcB#gF&jI)nM1qP5uT8xWcB%iI)mL0pP4tS7wWbAZeD%iH(lL0pO3sS7wVazZeD$hG(lK+oO3sR6vVazYdC$hG*kK+oN2rR6vU9yYdC!gF*kJ-nN2rQ5uU9yXcB!gF&jJ-nM1qQ5uT8xXcB#fE&jI)mM1qP4tT8xWbA#fE%iH)mL0pP4tS7wWbAZeD%iH(lL0pO3sS7wVazZeD$hG(lK+oO3sR6vVazYdC$hG*kK+oN2rR6vU9yYdC!gF*kJ-nN2uT8yXcB#fF&jI)nM1qP4uT8xWbB#fE%jI)mL0qP4tS7xWbAZeE%iH(mL0pO3tS7wVaAZeD$iH(lK+pO3sR6wVazYdD$hG*lK+oN2sR6vU9zYdC!gG*kJ-oN2rQ5vU9yXcC!gF&kJ-nM1rQ5uT8yXcB#fF&jI)nM1qP4uT8xWbB#fE%jI)mL0qP4tS7xWbAZeE%iH(mL0pO7wVazYeD$hG(lK+oN3sR6vUazYdC$hG*kJ+oN2rQ6vU9yXdC!gF*kJ-nM2rQ5uT9yXcB!gF&jI-nM1qP5uT8xWcB#fE&jI)mL1qP4tS8xWbAZfE%iH)mL0pO4tS7wVbAZeD%iH(lK0pO3sR7wVazYeD$hG(lK+oN3sR6vUazYdC$hG*kJ+oN2rQ6vU9yXgF&jJ-nM1qQ5uT8x