臨床免疫學(xué)與檢驗英語教案-肝功能

教案2013~2014學(xué)年第1學(xué)期課程名稱臨床免疫學(xué)與檢驗開課學(xué)院檢驗學(xué)院開課教研室臨床免疫學(xué)檢驗授課教師職稱技師授課班級2010級醫(yī)學(xué)檢驗學(xué)生人數(shù)102醫(yī)學(xué)院教務(wù)處制醫(yī)學(xué)院教案課程名稱ClinicalImmunology&ImmunologicalLaboratoryScience授課題目（章節(jié)或主題）ELISADetecthepatitisbvirus授課教師所屬二級學(xué)院KingmedCollegeofLaboratoryScience所屬教研室DepartmentofClinicalImmunology&ImmunologicalLaboratoryScience職稱Technician授課時間October11,2013,Thursday,Thesixthweek,section1,thefirstclass授課時數(shù)4classes授課班級Undergraduatelevel2010教學(xué)課型Theexperimentalclass教材名稱、作者、出版社及出版時間ChangqianZeng&ZhihuaTao.ExperimentGuideofClinicalImmunology&ImmunologicalLaboratoryScience.Chinesemedicinescienceandtechnologypress,2010,2.教學(xué)目標(biāo)與要求：1、fullyUnderstandtheprinciple,operationprocessandattentionissuesoftheELISAmethodtodetectHepatitisBvirus.2、knowtheclinicalsignificanceofdetectionofHepatitisBvirus。主要知識點、重點與難點：Themainpoint：theprinciple,operationprocessofthedoubleantibody/antigensandwichmethod、neutralcompetitivemethod、competitiveinhibitionmethod.Thedifficultpoint:theoneisthecharacteristicsofneutralCompetitivemethodandcompetitiveinhibitionmethod,theotheroneistheclinicalsignificanceofdifferentresults.教學(xué)方法（請打√選擇）：講授法□√討論法□√演示法□√自學(xué)輔導(dǎo)法□練習(xí)法(習(xí)題或操作)√讀書指導(dǎo)法□□案例法□□教學(xué)媒體（請打√選擇）：教材□板書□√實物□√標(biāo)本□√掛圖□模型□投影□幻燈□錄像□CAI（計算機輔助教學(xué)）□√教學(xué)過程設(shè)計（包括講授內(nèi)容、講授方法、時間分配、媒體選用、板書設(shè)計等）：講授內(nèi)容講授方法、時間分配、手段一、Reviewtheliverfunction:Metabolism;Biotransformation;SecretionandExcretion.3minutesIntroducingthemaincontent二、introducethebilirubinmetabolismmechanism:1、Conceptionofthreedifferentkindsofbilirubin;totalserumbilirubin,conjugatedbilirubin,unconjugatedbilirubin.2、Themetabolismmechanismofthreedifferenttypesofjaundice;①Normalbilirubinmetabolism②Hemolyticjaundicemechanismdiagram③Hepatocellularjaundicemechanismdiagram④Cholestaticjaundicemechanismdiagram2minutesThepictures

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students

tounderstandthepoint.3minutes5minutes5minutes5minutes三、Theprincipleofthedetectionofconjugatedbilirubinandtotalserumbilirubin.1、Conjugatedbilirubin:DiazoniumSaltStableDiazonium-BilirubinAlkalineSodiumTartrateUnstableDiazoniumSaltStableDiazonium-BilirubinAlkalineSodiumTartrateUnstableDiazonium-BilirubinConjugatedBilirubinTotalserumbilirubin:ConjugatedConjugatedBilirubinDiazoniumSaltUnconjugatedBilirubinCaffeinesodiumBenzoateUnstableDiazonium-BilirubinAlkalineSodiumTartrateStableDiazonium-Bilirubin5minutes四、DetectionStepsandattentionnotes:STBtubeCBtubeBlanktubenoticeSampleAfterreacting1min,CBtubeadded0.05mlInhibitors.Putallthetubesundertheroomtemperaturefor10min.unit：（ml）Caffeinereagent1.61.6Distilledwater1.55Sulfonicacid0.4Diazoreagent0.40.4Sodiumazide0.05PotassiumsodiumtartrateUseblanktubeasstandardandadjustabsorbancetobe“0”.ReadabsorbanceofSTBandCBatwavelengthof600nm.10minutes五、resultandclinicalsignificanceSTB（umol/L）=STB（A）×228CB（umol/L）=CB（A）×228UCB=STB-CB2minutes提問、課堂討論等師生互動的設(shè)計：1、Accordingtotheresult,whichtypeofJaundicedoesthispatienthave?2、HowtodistinguishthethreedifferentkindsofJaundice?教學(xué)小結(jié)、復(fù)習(xí)思考及作業(yè)題布置：summarize：1、Reviewtheliverfunction.2、Introducetheconceptionofthreedifferentkindsofbilirubinandthemetabolismmechanism:3、Theprincipletheexperiment.4、DetectionStepsandattentionnotes.5、ResultandclinicalsignificancesQuestures：1、Accordingtotheresult,whichtypeofJaundicedoesthispatienthave?2、HowtodistinguishthethreedifferentkindsofJaundice?教學(xué)中的創(chuàng)新點（加強基礎(chǔ)與臨床聯(lián)系、外語運用、啟發(fā)學(xué)生思維、指導(dǎo)學(xué)生自學(xué)、介紹學(xué)科新進展等方面）：1、Focusontheconnectionoftheoryandpractice.Questionsandinteractiveteachingwillinspirestudentstothink.2,Usingthegraphicsandanimationtodescribetheexperimentalprinciplewhichwillhelpthestudentstounderstandtheteachingcontent.3、Basedonthereality,weinterpretMetabolismofbilirubinandthethreedifferenttypesofjaundice.Connectingwithclinic,letstudentsknowtheclinicalsignificanceandhelpstudentsunderstandandmemorytheknowledge.參考資料（包括輔助教材、參考書、文獻等）：1、ClinicalLaboratoryDiagnostic(au:LotharThomas)2、Fang,Liu，JulienSegbo,JunhongLiu&XiaolianZhang.ProfessionalEnglishforLaboratoryMedicalScience,WuhanUniversityPress,2012,7.教研室意見教研室主任簽章：年月日教學(xué)后記（即教學(xué)實施情況總結(jié)分析，在課程結(jié)束后填寫）Mostofstudentscanmasterthepri