博士入學(xué)復(fù)試匯報(bào)85

DoctoralCandidatesEntrance

Examinationof

*****UniversityCandidate:******ApplyingMajor:Microbialandbiochemical

pharmacyOutlineEducationExperience

ResearchExperiencePublicationsMasterResearchDoctoralResearchPlanEducationExperiencePeriodMajordegreeSchool二零一一.零九-二零一五.零七BiologicalEngineeringbachelordegree***二零一五.零九-MicrobialandbiochemicalpharmacyMasterdegree****EducationExperienceTraditionalChineseMedicineBureauofGuangdongProvince(NO.二零一四一零六五),NaturalScienceFoundationofGuangdongProvince(NO.二零一七A三二二一七零零七)NationalUniversityStudentInnovationProgram.ProfessionalThird-classScholarshipfrom二零一五-二零一八academicyearsAwardsPublications******,

EnzeYang,LianghuaShen,DeweiNiu,Mason

Breitzig,LeeCharlesTan,FengWang*.Thenoveltruncatedisoformofhumanmanganesesuperoxidedismutasehasadifferentialroleinpromotingmetastasisoflungcancercells.CellBiologyInternational.

Accepted.doi:一零.一零零二/cbin.一零九七二.******,

LianghuaShen,SijiaLei,Mason

Breitzig,LeeCharlesTan,QingZheng,HanxiaoSun,FengWang*.PTBP一enhancesexon一一askippinginMenapre-mRNAtopromotemigrationandinvasioninlungcarcinomacells.TheJournalofBiologicalChemistry.Majorrevision.JBC/二零一八/零零二六二七R一.DeweiNiu,******,ShanzeYi,FengWang*.Genecloning,proteinexpressionandfunctionalanalysisofatype三metallothioneingenefromSonneratiaalbawithbiosorptionpotential.PolishJournalofEnvironmentalStudies,Accepted.PJOES-零零六四七-二零一七-零二.MasterResearchPTBP一enhancesexon一一askippinginMenapre-mRNAtopromotemigrationandinvasioninlungcarcinomacellsBackgroundObjectivesTechnologyMethodsResultsConclusionsBackgroundSevenalternativesplicing(AS)subgroups:Exonskippingaccountsfornearly四零%ofASevents;alternative三′splicesite(三′SS)selection(一八.四%)and五′SSselection(七.九%);intronretentionaccountsforlessthan五%;mutuallyexclusiveexons,alternativepromoterusage(三′UTR)andalternativepolyadenylation(五′UTR)arenotfrequent.BackgroundHumanorthologofmammalianenabled(Mena)regulatescellfunctionmainlybyantagonizingactincappingproteins,hasbeenshownmultiplesplicevariantsinhumantumorcells.MenaINVforinvasivefeatureMena一一aforepithelial-specificfeature

BackgroundTwomainASregulatorsheterogeneousnuclearribonucleoprotein(hnRNPs)andserine/arginine-rich(SR)proteins.PolypyrimidineTract-BindingProtein一(PTBP一,alsoknownashnRNPI)bindspreferentiallytopyrimidine-richsequences.

ObjectivesTheexactmechanismmediatedMenaAShasnotbeenelucidated.ThepurposeofthisstudyistoexplorethemolecularmechanismofPTBP一inregulatingMenaalternativesplicingandroleofPTBP一inlungcarcinomacellsmetastasis.RegulatedbyPTBP一?TechnologyMethods

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results一.一.PTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesA.PTBP一expressionwaselevatedinLUADtissues(N=五一五)comparedwithnormallungtissues(N=五九)accordingtoTCGAdatabase(p<零.零一);B.Kaplan-MeierplotsofpatientswithLUADaccordingtohigh(N=一二七)andlow(N=三七五)PTBP一expressionfromtheTCGAdatabaseandcomparedbypairedt-test,p<零.零一.AB

Results一.二.PTBP一ishighlyexpressedinmetastaticlungcancercells(九五-D)

Results一.三.UpregulationofPTBP一isassociatedwithTGFβinducedEMTprogress

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results二.一.OverexpressedPTBP一promoteslevelsofEMT-relatedproteinsinlungcancercells

Results二.二.OverexpressedofPTBP一promotesmigration

oflungcancercells

Results二.三.KnockdownofPTBP一inhibitslevelsofEMT-relatedproteinsinlungcancercells

Results二.四.KnockdownofPTBP一inhibitsmigrationoflungcancercells

Results二.五.PTBP一promotesinvasion

oflungcancercells

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results三.一.MenaINV

iselevatedinthehighlymetastaticlungcancercellsAB

Results三.二.PTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercells.

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results四.一.DiagramoftheMenapre-mRNAminigenespanningfromexon一零toexon一二B

Results四.二.PTBP一enhancesexon一一askippinginMena

minigene

Results四.三.PTBP一bindstobothupstreamanddownstreamintronsequencesinMenapre-mRNABCA.

SchemeofwildMenaminigeneandmutantMenaminigene.TheunderlinedsequencesareconsensusPTBP一bindingsites.B.RT-PCRassayswereperformedtodetectthechangesofexon一一askippingofwildandmutantMenaminigenesinPTBP一overexpressedA五四九cells.

C.

WesternblotanalysisofRNA-pulldownassaysshowedthatthechangesofinteractionsbetweenPTBP一andbiotin-RNAoligomerscontainingpotentialPTBP一bindingsites.

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results五.一.PTBP一-mediatedmigration

of九五-DcellsispartiallydependentonMenaINV

Results五.三.ThefilopodiaformationwasregulatedbyPTBP一anddependedonchangeofMenaINV.Thefieldsofviewswerecapturedat一零×四零magnification(Scalebars:一零μm)

ConclusionDoctoralResearchPlanMolecularMechanismforTumorMetastasisRegulatedby******BackgroundObjectivesResearchContentsTechnologyMethodsExpectedResults

Background******hasbeenreportedtobeeffectiveintreatingOsteoarthritis(OA)andinhibitingtumorcellsproliferation.Itisnotclearwhether******inhibitstumormetastasisanditspotentialmolecularmechanisms.ObjectivesTostudytheeffect******on

theregulating

tumormetastasisinseveralcancercells.Toexplorethemolecularmechanismsofinhibitingmetastasisby******,andprovidethebasicdatafortheclinicalapplicationof******.ResearchContentsThemetastatic

modeinvitro

is

inducedbytransforminggrowthfactor-β(TGF-β).Theadhesionandproliferationofcancercellstreatedwith******areevaluated.Wound-healingandtranswellassaysareperformedtostudytheeffect******

ontheregulating

tumormigrationandinvasion.

Thetumormetastasismodeinvivoisestablishedinnudemouse.TranscriptomeandProteomicsareusedtodetecttheexpressionsoftranscriptionfactorsorpathway-relatedproteins.Inhibitorsareusedtodemonstratethemolecularmechanismsofmetastasisinhibitedby******.TechnologyMethodsMolecularMechanismforTumorMetastasisRegulatedbyXanthanGumRegulationofTumorMetastasisStudyofthemolecularmechanismInvitroInvivoCellviabilityCellcycleCelladhesionMigrationInvasionTumorgrowthTumormetastasisImmunohistochemistryVivoimagingTranscriptomeProteomicsWesternblotqPCRInhibitorImmunofluorescence******ExpectedResults

******

caneffectivelyinhibitthemetastasisoftumorcells.Thepotentialmolecularmechanismscanbeelucidated.大學(xué)博士入學(xué)復(fù)試報(bào)告學(xué)校:XX大學(xué)

大學(xué)姓名:XX

報(bào)考導(dǎo)師:XX報(bào)告內(nèi)容一二三個(gè)人簡(jiǎn)歷碩士階段研究情況博士生階段學(xué)術(shù)規(guī)劃一個(gè)人簡(jiǎn)歷第部分個(gè)人簡(jiǎn)歷個(gè)人概況姓名:

籍貫:山東青島畢業(yè)學(xué)校:中國(guó)藥科大學(xué)報(bào)考專業(yè):藥理學(xué)報(bào)考方向:生物藥物的藥理活性年齡:二六應(yīng)屆:是個(gè)人簡(jiǎn)歷本科碩士二零零七.九至二零一一.六安徽理工大學(xué)化工學(xué)院化學(xué)工程與工藝專業(yè)讀本科

優(yōu)秀學(xué)生干部三好學(xué)生校獎(jiǎng)學(xué)金政治輔導(dǎo)員助理

二零一一.九至今安徽理工大學(xué)化工學(xué)院化學(xué)工程專業(yè)讀碩士

三好學(xué)生校獎(jiǎng)學(xué)金個(gè)人簡(jiǎn)歷學(xué)習(xí)成績(jī)碩士主干課程成績(jī)優(yōu)秀,加權(quán)平均分九零.二,GPA三.六一.一零六E一零門(mén)課程不低于九零分藥理學(xué)進(jìn)展、細(xì)胞分子藥理學(xué)、高級(jí)毒理學(xué)、科研設(shè)計(jì)、論文寫(xiě)作……六門(mén)課程不低于八零分細(xì)胞生物學(xué)、臨床藥理學(xué)、生物數(shù)學(xué)模型、SPSS數(shù)理統(tǒng)計(jì)……英語(yǔ)免修通過(guò)國(guó)家大學(xué)英語(yǔ)四六級(jí)考試通過(guò)上海中級(jí)口譯個(gè)人簡(jiǎn)歷本科階段三次獎(jiǎng)學(xué)金二零一零.零九

學(xué)校二等獎(jiǎng)學(xué)金二零一一.零九

學(xué)校一等獎(jiǎng)學(xué)金二零一二.零九

學(xué)校三等獎(jiǎng)學(xué)金碩士階段二次獎(jiǎng)學(xué)金二零一三.零九

新生獎(jiǎng)學(xué)金二零一五.零九

研究生國(guó)家獎(jiǎng)學(xué)金二碩士階段研究狀況第部分碩士階段研究狀況一.碩士研究方向:生物質(zhì)催化熱解轉(zhuǎn)化.零一零二零三零四二.參加過(guò)基金項(xiàng)目:安徽省教育廳自然科學(xué)基金重點(diǎn)項(xiàng)目（KJ****A零七零）—農(nóng)林廢棄物兩段催化氣化制富氫燃?xì)庋芯?三.在北大核心期刊《可再生能源》第一作者發(fā)表論文:《三種過(guò)渡金屬氧化物對(duì)生物質(zhì)微波催化熱解產(chǎn)物影響》.四.撰寫(xiě)專利兩篇:《一種可以降低工業(yè)堿木質(zhì)素?zé)峤鉁囟鹊难趸瘎贰兑环N可降低生物油含氧量提高芳烴含量的催化劑》碩士階段研究狀況零一碩士論文題目:三種過(guò)渡金屬氧化物對(duì)生物質(zhì)微波快速催化熱解研究以HZSM-五為載體,利用共沉淀法制備三種過(guò)渡金屬氧化物催化劑,考察其對(duì)生物質(zhì)快速熱解的催化效果.零二催化劑Fe二O三/HZSM-五失活和再生進(jìn)行研究.零三利用均勻沉淀法合成nano-Fe二O三/HZSM-五,并與普通的Fe二O三/HZSM-五催化效果作對(duì)比.碩士階段研究狀況實(shí)驗(yàn)裝置圖一-氮?dú)馄?；?壓力表；三-轉(zhuǎn)子流量計(jì)；四-微波高溫爐；五-石英反應(yīng)器；六-直冷凝管；七-三口燒瓶；八-冰浴杯；九-鐵架臺(tái)；一零-U形管；一一-集氣袋碩士階段研究狀況二.一MOx/HZSM-五的合成與表征（a）Fe二O三/HZSM-五(b)Co三O四/HZSM-五(c)NiO/HZSM-五SEM表征經(jīng)分析,催化劑較好的負(fù)載在載體的表面XRD表征碩士階段研究狀況abcd三種催化劑的催化熱解效果:(a)三種催化劑均具有良好的脫氧效果,可提高生物油品質(zhì)；(b)熱解液相產(chǎn)物中芳香類化合物都大幅提高,其中Fe二O三/HZSM-五催化作用最明顯,最高產(chǎn)率可達(dá)四八.二零%；(c)三種催化劑都能夠促進(jìn)G型與S型酚轉(zhuǎn)化生成結(jié)構(gòu)更穩(wěn)定的H型酚,NiO/HZSM-五的催化效果更明顯.(d)Fe二O三/HZSM-五和NiO/HZSM-五可分別促進(jìn)生物質(zhì)向糠醛和苯酚的定向熱解,產(chǎn)率分別可達(dá)一零.八六%和一四.六零%.碩士階段研究狀況二.二Fe二O三/HZSM-五失活和再生積碳量分析XRD分析碩士階段研究狀況SEM分析新鮮Fe二O三/HZSM-五失活Fe二O三/HZSM-五經(jīng)分析后得出,催化劑失活主要是因積碳引起,空氣燒炭法對(duì)其再生,再生催化劑仍有良好催化效果碩士階段研究狀況XRD分析SEM分析二.三Nano-Fe二O三/HZSM-五的合成與表征用均勻沉淀法合成Nano-Fe二O三/HZSM-五.根據(jù)衍射峰半高寬和Scherrer公式D=零.八九λ/β(cosθ)估算Fe二O