pcombia載體圖譜

1、pCAMBIA1305.1 and pCAMBIA1305.2This vector series is based on a new reporter gene GUSPlusä. The novel reporter enzyme has improved enzymatic characteristics, and can be more efficiently secreted than E. coli GUS when fused with a signal peptide. pCAMBIA1305.1 and pCAMBIA1305.2CAMBIAs GUSPlus

2、28; is a new reporter gene isolated from Staphylococcus sp with superior properties to E.coli GUS§ Better catalytic activity for more rapid detection of GUS activity.§ Greater stability at 60°C and in the presence of fixatives such as formaldehyde and glutaraldehyde for robust, cell-s

3、pecific detection of gene activity in situ.§ A version with the rice glycine-rich protein signal peptide for extracellular secretion providing rapid, in vivo gus assays.GUSPlusä has been tested extensively in transgenic rice, and the gene has been codon optimised for high expression in pla

4、nts. The version in pCAMBIA1305.2 with a signal peptide provides an alternative to GFP, offering sensitive in vivo observation of gene activity without the need for UV detection.GUSPlusä is available in two vector formats:§ pCAMBIA1305.1 (Genbank acc nr AF354045). This vector is based on p

5、CAMBIA1300 in which E. coli gusA has been replaced by GUSPlusä. It is compact binary vector with a pBR322 ori for high copy replication in E.coli and a broad host range ori for low copy, stable replication in Agrobacterium. It contains the hygromycin resistance gene (hpt II) for selection in pl

6、ants. The GUSPlusä gene contains the intron from the castor bean catalase gene to ensure detection of plant-specific glucuronidase expression.§ pCAMBIA1305.2 (Genbank acc nr AF354046). Same as pCAMBIA1305.1 except for the presence of the rice glycine-rich protein signal peptide sequence (G

7、RP) inserted between the Nco I and Bgl II sites.General featurespCAMBIA 1305.1pCAMBIA 1305.2pUC18 multiple cloning siteBlue/white b-galactosidase screeningHigh copy plasmid in E.coliTriparental mating feasibilityGUSPlusä replacement of gusA as reporter geneHexa-His GUSPlusä purificationGUSPlusä secreted to apoplastic spaceCan be maintained in Agrobacterium without selectionBacterial antibiotic selectionPlant antibiotic selectionYesYesYesYesYesYesNoYesKanamycinHygromycinYesYesYesYesYesYesYesYesKanamycinHygromycinContact: Vector inquiries.CAMBIA, GPO Box 3200, Canbe