補陽還五湯藥浴對大鼠周圍神經(jīng)損傷再生影響的實驗研究

1、補陽還五湯藥浴對大鼠周圍神經(jīng)損傷再生影響的實驗研究         09-09-08 14:31:00     作者：石杜鵑，凌麗，薛金偉    編輯：studa20【摘要】  目的研究補陽還五湯藥浴對周圍神經(jīng)損傷后修復(fù)的作用。方法采用雌性Wistar大鼠90只， A組為補陽還五湯藥浴組，B組為補陽還五湯口服組，C組為單純端端縫合組，每組30只。造模采用大鼠坐骨神經(jīng)橫斷損傷模型，造模成功后7 d起，A組大鼠放入補陽還五湯溫熱藥

2、液中游泳，隔日1次，5 min/次； B組用補陽還五湯制劑灌胃，隔日1次，3 ml/次；C組大鼠進行單純端端縫合。3組均于術(shù)后6周取材進行電生理學、組織形態(tài)學觀察。結(jié)果補陽還五湯藥浴可以明顯減少神經(jīng)損傷后運動神經(jīng)元和感覺神經(jīng)元的死亡，防止支配肌肉的萎縮變性，促進損傷后功能的恢復(fù)。結(jié)論補陽還五湯藥浴可以在神經(jīng)元、損傷局部、終末效應(yīng)器多個平面、多個角度對神經(jīng)修復(fù)產(chǎn)生積極作用。 【關(guān)鍵詞】  周圍神經(jīng)；損傷與再生；補陽還五湯；藥浴Abstract：ObjectiveTo study the effect of Buyang Huanwu Decoction medicated bath o

3、n neurogenesis after the peripheral nerve injury.Methods90 wistar rats were randomly divided into 3 groups, group A was Buyang Huanwu Decoction medicated bath ,group B was Buyang Huanwu Decoction administration, group C simply had an end-to-end sutured operation serve as control. The model of ischia

4、dic nerve transsection were adopted. After 7 days, wistar rats of group A  swam in the warm Buyang Huanwu Decoction physic liquor, 5 minutes a time every other day. The intragastric administration was given in group B 3 ml a time every other day. Group C simply had an end-to-end sutured operati

5、on. Electrophysiology and histomorphology were detected after 6 weeks. ResultsThe Buyang Huanwu Decoction medicated bath could obviously reduce the death number of motor neurons and sensory neurons after nerve injury, prevent the atrophy and degeneration of gastrocnemius muscle and promote the funct

6、ional recovery after injury. ConclusionThe Buyang Huanwu Decoction medicated bath may play an active role in the nerve repair of neuron, damage region and ending effective apparatus from multiple facts.Key words：Peripheral nerve;  Injury and restore;  Buyang Huanwu Decoction；  Medicat

7、ed bath    周圍神經(jīng)損傷屬于細胞損傷的范疇1，其再生的基礎(chǔ)和臨床研究雖取得了長足的進步，但目前周圍神經(jīng)損傷修復(fù)后功能恢復(fù)仍欠理想。究其原因，對于每一條神經(jīng)纖維來說，神經(jīng)元、神經(jīng)干中的支持細胞及其終末器官是互相營養(yǎng)的。周圍神經(jīng)干損傷后，其上位神經(jīng)元、整個神經(jīng)干及終末器官均會發(fā)生變性甚至壞死。所以，周圍神經(jīng)損傷的修復(fù)也是一個綜合的修復(fù)過程。近年來，中醫(yī)中藥以其多靶點、多途徑的綜合療效優(yōu)勢2愈來愈受到人們的重視。神經(jīng)損傷屬于中醫(yī)的“痿證”，表現(xiàn)為肢體功能障礙，肌肉萎縮，感覺消失，治療上應(yīng)補氣活血通絡(luò)，補陽還五湯在臨床治療和我們的前期研究中有良好的作用。因此，我

8、們設(shè)計了動物實驗來進一步研究補陽還五湯藥浴及口服對周圍神經(jīng)再生的影響。1  材料與方法1.1 材料實驗于2006-03-07在黑龍江中醫(yī)藥大學實驗中心完成。雌性Wistar大鼠，90只，體重220250 g，購自黑龍江中醫(yī)藥大學動物實驗中心。中藥煎劑: 生黃芪120 g，當歸尾6 g，赤芍6 g，川芎3 g，紅花3 g，地龍3 g，桃仁3 g。方藥組成采用補陽還五湯原方，5倍劑量為1劑。混合均勻，煎煮前預(yù)先用清水浸泡20 min，加水量約5 000 ml，煮沸后文火再煎約20 min，煎得劑量約3 0003 500 ml，自然冷卻至微溫，進行灌胃或藥浴。中藥材及煎制均由黑龍江中醫(yī)藥大

9、學附屬第一醫(yī)院煎藥室提供。1.2  方法按手術(shù)先后順序隨機分成A、B、C 3組，A組為補陽還五湯藥浴組，B組為補陽還五湯口服組。C組為單純端端縫合組，每組30只。造模采用大鼠坐骨神經(jīng)橫斷損傷模型：右坐骨神經(jīng)于坐骨結(jié)節(jié)下0.5 cm水平橫行切斷，用9/0無創(chuàng)尼龍縫合線均勻縫合神經(jīng)外膜3針。造模成功后7 d起，A組大鼠放入補陽還五湯溫熱藥液中游泳，隔日1次，5 min/次；B組用補陽還五湯制劑灌胃，隔日1次，3 ml/次；C組大鼠進行單純端端縫合。3組均于術(shù)后6周取材進行電生理學、組織形態(tài)學觀察。自制大鼠行走箱。術(shù)后3周起，隔日進行SFI測定。碳素墨水刷染大鼠雙后足，放在底面鋪設(shè)白紙的行走箱通道上行走，可清晰記錄大鼠行走過程中雙側(cè)后