中藥指紋圖譜研究

1、中藥指紋圖譜研究 逍遙丸中甘草的鑒別（1.陰性對照、2.樣品 3.對照藥材） 1 2 3甘草的來源：甘草Glycyrrhiza uralensis Fisch.脹果甘草 Glycyrrhiza inflata Bat. 光果甘草 Glycyrrhiza glabra L. 的干燥根及根莖 3.建立中藥指紋圖譜的目的和意義建立中藥指紋圖譜的目的和意義中藥的特點：中藥的特點： （1）中藥化學(xué)成分的多樣性與復(fù)雜性； （2）中藥藥效成分的非單一性； （3）中藥質(zhì)量的不穩(wěn)定性。 3.建立中藥指紋圖譜的目的和意義建立中藥指紋圖譜的目的和意義 （1） 對中藥質(zhì)量控制的補充和提高：對中藥質(zhì)量控制的補充和提高：

2、 現(xiàn)有質(zhì)量控制模式：以1個或1個以上成分為控制指標(biāo)，難以準(zhǔn)確、全面地評價中藥質(zhì)量。 指紋圖譜從整體上評價中藥的內(nèi)在質(zhì)量，已為國際社會所認(rèn)可，有利于中藥及產(chǎn)品進(jìn)入國際市場。3.中藥指紋圖譜的研究目的意義中藥指紋圖譜的研究目的意義 （2）提高中藥質(zhì)量的穩(wěn)定性）提高中藥質(zhì)量的穩(wěn)定性 控制原料、中間體、成品的一致性，減少批間差異，提高中藥質(zhì)量的穩(wěn)定性。樣品編號樣品編號藥材產(chǎn)地藥材產(chǎn)地來源來源1湖北通城湖北通城GAP基地基地菝葜菝葜Simlia china L.2湖南靖州湖南靖州菝葜菝葜S. china L.3安徽墳天山安徽墳天山菝葜菝葜S. china L.4廣西賓陽廣西賓陽菝葜菝葜S. china

3、L.5廣東省從化縣廣東省從化縣菝葜菝葜S. china L.6重慶市馬腳山重慶市馬腳山菝葜菝葜S. china L.7浙江溫州市永嘉縣上塘鎮(zhèn)浙江溫州市永嘉縣上塘鎮(zhèn)菝葜菝葜S. china L.8福建仙游福建仙游菝葜菝葜S. china L.9湖南岳陽湖南岳陽菝葜菝葜S. china L.10江西樟樹市讓山江西樟樹市讓山菝葜菝葜S. china L. 11江西弋陽江西弋陽小果菝葜（小果菝葜（S.davidiana A.DC）12湖南長沙湖南長沙長托菝葜（長托菝葜（S.ferox Wall.ex Kunth）13湖北麻城湖北麻城黑果菝葜黑果菝葜(S.glauco-china Warb.)14湖北咸

4、寧湖北咸寧土茯苓土茯苓(S.glabra Roxb.) 精密稱取黃杞苷對照品適量，加甲醇制成每ml含50g的溶液。峰號平均值RSD()10.2750.2760.2730.2730.2750.274 0.49 20.3940.3960.3960.3930.3960.395 0.36 30.4880.490.4910.4860.490.489 0.41 40.8010.8040.8030.80.8020.802 0.20 50.8520.8550.8550.8520.8530.853 0.18 60.9200.920.9180.9190.9210.920 0.12 7（S）1.0001.0001.

5、0001.0001.0001.0000.00 81.2691.2651.2551.2681.2711.266 0.50 91.3421.3531.3551.3531.3481.350 0.39 101.7111.7081.6961.7071.7011.705 0.35 111.7771.7831.7861.7821.7781.781 0.21 121.8251.8311.8351.8291.8251.829 0.23 峰號平均值RSD（）10.7500.7570.7750.7730.7840.768 1.81 20.1770.1760.1690.1750.1760.175 1.84 30.35

6、90.3590.3620.3590.3600.360 0.36 40.2310.2330.2200.2250.2340.229 2.60 50.2280.2210.2210.2240.2260.224 1.38 61.4531.4611.4321.4621.4881.459 1.38 7（S）1.0001.0001.0001.0001.0001.000 0.00 87.8627.8567.8407.8417.8407.848 0.13 90.2540.2570.2540.2550.2530.255 0.60 101.2701.2741.2721.2741.2741.273 0.14 110.2

7、980.2910.2900.2940.2960.294 1.14 120.5780.5780.5740.5780.5770.577 0.30 峰號峰號平均值平均值RSD(RSD() )1 10.2750.2750.2760.2760.2750.2750.2730.2730.2780.2780.275 0.275 0.66 0.66 2 20.3940.3940.3960.3960.3960.3960.3960.3960.3940.3940.395 0.395 0.28 0.28 3 30.4880.4880.490.490.490.490.4910.4910.4890.4890.490 0.4

8、90 0.23 0.23 4 40.8010.8010.8030.8030.8020.8020.8030.8030.8030.8030.802 0.802 0.11 0.11 5 50.8520.8520.8550.8550.8550.8550.8530.8530.8520.8520.853 0.853 0.18 0.18 6 60.920.920.9190.9190.920.920.9210.9210.920.920.920 0.920 0.08 0.08 7 7（S S）1.0001.0001.0001.0001.0001.0001.0001.0001.0001.0001.000 1.00

9、0 0.00 0.00 8 81.2691.2691.2641.2641.2651.2651.2651.2651.2651.2651.266 1.266 0.15 0.15 9 91.3421.3421.3531.3531.3551.3551.3531.3531.3481.3481.350 1.350 0.39 0.39 10101.7111.7111.7061.7061.7061.7061.7061.7061.7061.7061.707 1.707 0.13 0.13 11111.7771.7771.7821.7821.7781.7781.7811.7811.7821.7821.780 1.

10、780 0.13 0.13 12121.8251.8251.831.831.831.831.8311.8311.8311.8311.829 1.829 0.14 0.14 峰號峰號平均值平均值RSD()10.750.7590.760.7630.7740.761 1.14 20.1770.1750.1790.1770.1770.177 0.80 30.3590.360.3590.3620.3590.360 0.36 40.2310.2230.220.2240.2240.224 1.80 50.2280.2240.2210.2270.220.224 1.58 61.4531.4611.4571.4

11、591.4621.458 0.25 7(S)1.0001.0001.0001.0001.0001.000 0.00 87.8627.8557.867.8497.8457.854 0.09 90.2540.2540.2560.2550.2530.254 0.45 101.271.2741.2721.2741.2731.273 0.13 110.2980.2930.290.2890.2970.293 1.38 120.5780.5750.5790.5780.5780.578 0.26 峰號峰號樣品樣品1234567(S)8910111210.7500.1770.3590.2310.2281.453

12、1.0007.8620.2541.2700.2980.57821.1791.4270.5271.3921.0901.8091.0007.9341.0211.6681.0090.51430.7820.1310.1650.3000.0851.0891.0006.2900.2050.4820.1430.18541.1600.2431.0750.6301.8051.8011.0007.9110.2870.7890.9820.25250.1980.8150.2030.3800.7211.0441.0006.2950.2960.6030.3380.07061.3931.6601.2961.8320.586

13、1.8121.0006.3170.2372.7041.0880.62371.2842.4331.7551.3242.0151.4771.0006.2990.9442.6810.7970.96781.0100.3361.8690.6611.6271.4571.0006.8670.5881.0330.5610.32290.8050.2761.6510.9340.4271.7911.0009.3810.5291.7770.2280.462102.5041.3192.6251.1501.9831.2161.0009.2850.8992.3311.0250.323峰面積占總峰面積大于峰面積占總峰面積大于

14、20，其差值不大，其差值不大于于20峰面積占總峰面積大于峰面積占總峰面積大于10，而小于，而小于20，其差值不大于其差值不大于25樣品編號樣品編號非共有峰面積非共有峰面積總峰面積總峰面積 比例（）比例（）17722.52657452.91220932.811140381.8831099.9530092.0741575.31148171.3751458.8443423.2961338.21031741.2973580.51271932.8182254.71310091.7291309.6779991.68101171.1423102.77水飛薊賓13.0min氧化白藜蘆醇 43.6min43.6min黃杞苷47.5min白藜蘆醇60.0min 樣品樣品 編號編號藥材藥材產(chǎn)地產(chǎn)地來源來