丙泊酚聯(lián)合骨髓間充質(zhì)干細(xì)胞移植脊髓損傷大鼠：改善后肢電生理功能作用優(yōu)于單獨(dú)用藥

1、丙泊酚聯(lián)合骨髓間充質(zhì)干細(xì)胞移植脊髓損傷大鼠：改善后肢電生理功能作用優(yōu)于單獨(dú)用藥來(lái)自中國(guó)河北醫(yī)科大學(xué)附屬唐山工人醫(yī)院王躍新所在科研團(tuán)隊(duì)一項(xiàng)最新的研究顯示，丙泊酚聯(lián)合骨髓間充質(zhì)干細(xì)胞移植治療可有效改善脊髓損傷大鼠后肢電生理功能，促進(jìn)運(yùn)動(dòng)功能的恢復(fù)，起到神經(jīng)保護(hù)作用。骨髓間充質(zhì)干細(xì)胞在一定條件誘導(dǎo)下具有自我更新、多向分化、遷移的能力，可在體外能夠分化培養(yǎng)擴(kuò)增為多種遺傳穩(wěn)定的組織和細(xì)胞，可作為理想的神經(jīng)元移植供體。移植的骨髓間充質(zhì)干細(xì)胞能在宿主體內(nèi)受損傷脊髓部位存活，產(chǎn)生并釋放趨化因子向損傷區(qū)域遷移、聚集，分泌多種生長(zhǎng)因子，抑制炎癥因子的表達(dá)，誘導(dǎo)損傷區(qū)域微血管的再生，減輕局部的繼發(fā)性炎癥反應(yīng)，分化成

2、神經(jīng)元和膠質(zhì)細(xì)胞，達(dá)到保護(hù)并促進(jìn)神經(jīng)元的再生重構(gòu)、治療脊髓損傷的目的。但目前的顯示單一的骨髓間充質(zhì)干細(xì)胞移植對(duì)神經(jīng)系統(tǒng)損傷的修復(fù)作用仍不理想。因此王躍新等擬研究神經(jīng)系統(tǒng)損傷后起神經(jīng)保護(hù)作用的丙泊酚與骨髓間充質(zhì)干細(xì)胞聯(lián)合干預(yù)對(duì)脊髓損傷的治療效果。王躍新等以打擊法建立脊髓損傷大鼠模型后，分別以骨髓間充質(zhì)干細(xì)胞移植和/或丙泊酚干預(yù)方法進(jìn)行治療。結(jié)果發(fā)現(xiàn)經(jīng)骨髓間充質(zhì)干細(xì)胞移植和/或丙泊酚治療4周后，脊髓損傷大鼠脊髓組織缺失及脊髓空洞明顯縮小，脊髓組織中PKH-26陽(yáng)性細(xì)胞和辣根過(guò)氧化物酶陽(yáng)性神經(jīng)纖維數(shù)量明顯增加，下肢感覺(jué)誘發(fā)電位及運(yùn)動(dòng)誘發(fā)電位的潛伏期明顯縮短，波幅增加，下肢運(yùn)動(dòng)功能明顯改善，且兩者聯(lián)合

3、效果優(yōu)于單獨(dú)使用。說(shuō)明移植細(xì)胞在脊髓損傷區(qū)存活，促進(jìn)了神經(jīng)纖維的修復(fù)及下肢感覺(jué)誘發(fā)電位及運(yùn)動(dòng)誘發(fā)電位的恢復(fù)。表明相關(guān)文獻(xiàn)發(fā)表于中國(guó)神經(jīng)再生研究（英文版）雜志2015年4月第4期。經(jīng)骨髓間充質(zhì)干細(xì)胞移植聯(lián)合丙泊酚治療4周后，脊髓損傷大鼠脊髓組織中辣根過(guò)氧化物酶陽(yáng)性神經(jīng)纖維數(shù)量明顯增加Article: " Propofol injection combined with bone marrow mesenchymal stem cell transplantation better improves electrophysiological function in the hindlim

4、b of rats with spinal cord injury than monotherapy," by Yue-xin Wang1, Jing-jing Sun2, Mei Zhang3, Xiao-hua Hou1, Jun Hong4, Ya-jing Zhou5, Zhi-yong Zhang1 (1 First Department of Orthopedics, Tangshan Workers Hospital Affliated to Hebei Medical University, Tangshan, Hebei Province, China; 2 Dep

5、artment of Neurology, Tangshan Union Medical College Hospital, Tangshan, Hebei Province, China; 3 Operating Room, Tangshan Workers Hospital Affliated to Hebei Medical University, Tangshan, Hebei Province, China; 4 Department of Neurosurgery, Tangshan Workers Hospital Affliated to Hebei Medical Unive

6、rsity, Tangshan, Hebei Province, China; 5 Department of Anesthesiology, Xingtai Peoples Hospital Affliated to Hebei Medical University, Xingtai, Hebei Province, China)Wang YX, Sun JJ, Zhang M, Hou XH, Hong J, Zhou YJ, Zhang ZY (2015) Propofol injection combined with bone marrow mesenchymal stem cell

7、 transplantation better improves electrophysiological function in the hindlimb of rats with spinal cord injury than monotherapy. Neural Regen Res 10(4):636-643.欲獲更多資訊：Neural Regen Res Propofol injection plus BMSCs transplantation vs. propofol injection for SCI repair According to a study appearing i

8、n Neural Regeneration Research (Vol. 10, No. 4, 2015), propofol injection combined with bone marrow mesenchymal stem cell (BMSCs) transplantation can effectively improve hindlimb electrophysiological function, promote the recovery of motor function, and play a neuroprotective role in spinal cord inj

9、ury (SCI).Under certain conditions, BMSCs have the ability to self-renew, multi-differentiate and migrate, are able to differentiate into various tissues and cells in vitro, and can be used as ideal donors for neuron transplantation. The transplanted BMSCs can survive in the host spinal cord, genera

10、te and release chemotactic factors which migrate toward and accumulate in the injured area, secrete various growth factors, inhibit the expression of inflammatory factors, induce the regeneration of microvessels in the injured area, alleviate local secondary inflammatory reaction, differentiate into

11、 neurons and glial cells, and protect and promote neuronal regeneration, thereby achieving the purpose of treating SCI. Simple BMSCs transplantation for repair of nervous system injury is not satisfactory. Yue-xin Wang et al. established rat models of SCI using the weight drop method and treated the

12、m with BMSCs transplantation and/or propofol injection to investigate the neuroprotective effects of these two interventions. They found that at 4 weeks after BMSCs transplantation and/or propofol injection, the cavity within the spinal cord was reduced, the numbers of PKH-26-positive cells and hors

13、eradish peroxidase-positive nerve fibers apparently increased in the spinal cord, latencies of somatosensory evoked potentials and motor evoked potentials in the lower extremity were noticeably shortened, amplitude was increased and lower extremity motor function was obviously improved. Moreover, th

14、e combined effects were better than cell transplantation or propofol injection alone. The above data suggest that the transplanted HBMSCs survived in the injured spinal cord, promoted the repair of nerve fibers, and contributed to the recovery of somatosensory evoked potentials and motor evoked pote

15、ntials. At 4 weeks after propofol injection plus BMSCs transplantation, the number of horseradish peroxidase-positive nerve fibers apparently increased in injured rat spinal cord tissue. Article: " Propofol injection combined with bone marrow mesenchymal stem cell transplantation better improve

16、s electrophysiological function in the hindlimb of rats with spinal cord injury than monotherapy," by Yue-xin Wang1, Jing-jing Sun2, Mei Zhang3, Xiao-hua Hou1, Jun Hong4, Ya-jing Zhou5, Zhi-yong Zhang1 (1 First Department of Orthopedics, Tangshan Workers Hospital Affliated to Hebei Medical Univ

17、ersity, Tangshan, Hebei Province, China; 2 Department of Neurology, Tangshan Union Medical College Hospital, Tangshan, Hebei Province, China; 3 Operating Room, Tangshan Workers Hospital Affliated to Hebei Medical University, Tangshan, Hebei Province, China; 4 Department of Neurosurgery, Tangshan Workers Hospital Affliated to Hebei Medical University, Tangshan, Hebei Province, China; 5 Department of Anesthesiology, Xingtai Peoples Hospital Affliated to Hebei Medical University, Xingtai, Hebei Province, China)Wang YX, Sun