抗體陽(yáng)性及陰性對(duì)照參考

1、常用抗體陽(yáng)性對(duì)照組織及判斷標(biāo)準(zhǔn)抗體名稱中杉編號(hào)對(duì) 照陽(yáng)性部位描述AMACRZA-0227正常腎臟+正常前列腺-細(xì)胞漿正常腎臟：近端小管的所有上皮細(xì)胞顯示明確的強(qiáng)烈的胞漿顆粒狀染色，這是判斷抗體質(zhì)量的可靠依據(jù)。但是必須注意使用非生物素檢測(cè)試劑。正常的前列腺作為該抗體的陰性對(duì)照，上皮細(xì)胞必須是陰性的，或僅有局灶性染色同時(shí)基質(zhì)細(xì)胞必須是陰性。SMAZM-0003肝臟+細(xì)胞漿肝臟是該抗體推薦的陽(yáng)性對(duì)照：肝臟的竇周細(xì)胞是驗(yàn)證該抗體質(zhì)量的可靠依據(jù)，這些細(xì)胞必須呈現(xiàn)盡可能強(qiáng)的胞漿陽(yáng)性反應(yīng)，而肝細(xì)胞漿或核不能有染色。由于脂褐素可見(jiàn)的顆粒狀反應(yīng)除外。Bcl-2ZM-0010ZA-536扁桃體+細(xì)胞漿扁桃體是合適

2、的陽(yáng)性對(duì)照：所有的外周T和B細(xì)胞強(qiáng)染色；生發(fā)中心散在的T細(xì)胞具有明確的陽(yáng)性反應(yīng)，而B(niǎo)細(xì)胞是陰性的；基底鱗狀上皮顯示至少弱至中等強(qiáng)度的明確的細(xì)胞漿反應(yīng)。BCL-6ZM-0011扁桃體+細(xì)胞核扁桃體是推薦的陽(yáng)性對(duì)照：生發(fā)中心的B細(xì)胞呈現(xiàn)強(qiáng)細(xì)胞核染色，更重要的是，鱗狀上皮的絕大多數(shù)細(xì)胞至少應(yīng)該是弱至中等強(qiáng)度的細(xì)胞核反應(yīng)。注意：有些克隆的抗體對(duì)H2O2敏感，應(yīng)注意過(guò)氧化氫的使用濃度。CEAZM-0061ZM-0062闌尾+肝臟驗(yàn)證抗體的特異性細(xì)胞漿闌尾是驗(yàn)證該抗體可靠性的陽(yáng)性對(duì)照：上皮細(xì)胞必須呈現(xiàn)明確的細(xì)胞漿反應(yīng)；肝臟可作為驗(yàn)證該抗體特異性的對(duì)照組織：膽小管或淋巴細(xì)胞必須沒(méi)有反應(yīng)。CD3ZA-0503

3、扁桃體+細(xì)胞膜/漿扁桃體為推薦的陽(yáng)性對(duì)照：散在的生發(fā)中心和套區(qū)T細(xì)胞必須呈現(xiàn)請(qǐng)明確的強(qiáng)染色，而B(niǎo)細(xì)胞陰性。該抗體并不能區(qū)分前T細(xì)胞淋巴瘤中的胞漿性CD3表達(dá)和外周T細(xì)胞淋巴瘤中的細(xì)胞膜型CD3表達(dá)。CD4ZA-0519扁桃體+細(xì)胞膜/漿扁桃體為推薦的陽(yáng)性對(duì)照：輔助/介導(dǎo)性T細(xì)胞需呈現(xiàn)強(qiáng)的胞膜染色，同時(shí)生發(fā)中心的巨噬細(xì)胞至少是弱至中等強(qiáng)度的明確的染色，后者驗(yàn)證該抗體的可靠的依據(jù)。注意：有些克隆的抗體對(duì)H2O2敏感，應(yīng)注意過(guò)氧化氫的使用濃度。CD8ZA-0508扁桃體+細(xì)胞膜/漿扁桃體為推薦的陽(yáng)性對(duì)照：散在和成簇的T細(xì)胞必須有明確的陽(yáng)性反應(yīng)，正常和腫瘤性的T細(xì)胞染色狀態(tài)無(wú)差別。CD10ZM-028

4、3ZA-0526扁桃體+細(xì)胞膜扁桃體是推薦的陽(yáng)性對(duì)照：所有的生發(fā)中心的B細(xì)胞必須呈現(xiàn)中等至強(qiáng)的細(xì)胞膜染色。推薦使用靈敏度更高的檢測(cè)試劑。CD20ZM-0039ZA-0549扁桃體+細(xì)胞膜/漿扁桃體是推薦的陽(yáng)性對(duì)照：套區(qū)和生發(fā)中心的B細(xì)胞應(yīng)盡可能強(qiáng)地呈現(xiàn)細(xì)胞膜陽(yáng)性反應(yīng)，而其它細(xì)胞（上皮細(xì)胞、肌細(xì)胞等）應(yīng)陰性反應(yīng)。CD23ZM-0273ZA-0516扁桃體+細(xì)胞膜扁桃體為推薦的陽(yáng)性對(duì)照：活化的套區(qū)B細(xì)胞（10-50%的細(xì)胞）必須呈現(xiàn)明確的中等至強(qiáng)的細(xì)胞膜染色，如果這些細(xì)胞是陰性或弱陽(yáng)性反應(yīng)，那么在慢性B淋巴細(xì)胞白血病B-CLLs中該抗體亦呈現(xiàn)陰性反應(yīng)或可疑的反應(yīng)。CD30ZM-0043扁桃體+細(xì)胞

5、膜/漿扁桃體為可靠的陽(yáng)性對(duì)照：活化的濾泡間和濾泡周?chē)腂和T細(xì)胞必須呈現(xiàn)明確的中等至強(qiáng)的細(xì)胞膜染色，同時(shí)可局灶性斑點(diǎn)樣反應(yīng)。這些細(xì)胞的染色強(qiáng)度和霍奇金淋巴瘤或胚胎癌中的腫瘤細(xì)胞是對(duì)等的。CD31ZM-0044肝臟+扁桃體+細(xì)胞膜肝臟為推薦的陽(yáng)性對(duì)照之一：肝竇內(nèi)皮細(xì)胞呈現(xiàn)明確的至少弱至中等強(qiáng)度的細(xì)胞膜為主的陽(yáng)性反應(yīng)，同時(shí)肝門(mén)脈處的大血管內(nèi)皮細(xì)胞呈強(qiáng)陽(yáng)性反應(yīng)，而肝細(xì)胞必須是陰性反應(yīng)。扁桃體為另一個(gè)推薦的陽(yáng)性對(duì)照：活化的B細(xì)胞和T細(xì)胞（尤其是套區(qū)的B細(xì)胞）呈現(xiàn)弱至中等強(qiáng)度的明確細(xì)胞膜染色，內(nèi)皮細(xì)胞呈強(qiáng)陽(yáng)性反應(yīng)。如果僅僅是大血管的內(nèi)皮細(xì)胞呈陽(yáng)性反應(yīng)，而活化的T/B細(xì)胞陰性反應(yīng)，則該抗體在血管肉瘤中的腫

6、瘤細(xì)胞中也會(huì)呈現(xiàn)假陰性反應(yīng)。CD34ZM-0046肝臟+闌尾+細(xì)胞膜/漿肝臟作為推薦的陽(yáng)性對(duì)照可以為抗體的質(zhì)檢提供最豐富的信息：門(mén)脈管道周?chē)膬?nèi)皮細(xì)胞呈現(xiàn)中等至強(qiáng)的細(xì)胞膜為主的陽(yáng)性反應(yīng)，而肝細(xì)胞必須是陰性反應(yīng)。 闌尾也可以作為陽(yáng)性對(duì)照：肌層中的Cajal細(xì)胞和固有層中小血管的內(nèi)皮細(xì)胞必須顯示中等至強(qiáng)的陽(yáng)性反應(yīng)，而上皮必須是陰性反應(yīng)。CD45 （LCA）ZM-0183肝臟+扁桃體輔助+細(xì)胞膜/漿肝臟為推薦的陽(yáng)性對(duì)照：淋巴細(xì)胞，尤其是Kupffer細(xì)胞呈明確的細(xì)胞膜反應(yīng)，可以驗(yàn)證抗體的反應(yīng)性，因?yàn)镵upffer細(xì)胞通常表達(dá)CD45蛋白比較低。 扁桃體可作為補(bǔ)充的陽(yáng)性對(duì)

7、照：所有的淋巴細(xì)胞必須是陽(yáng)性反應(yīng)。CD56ZM-0057扁桃體+細(xì)胞膜扁桃體為推薦的陽(yáng)性對(duì)照：濾泡間的NK細(xì)胞必須呈現(xiàn)強(qiáng)的以膜為主的反應(yīng)，并且陽(yáng)性反應(yīng)需在低倍鏡下清晰可見(jiàn)。外周神經(jīng)不可單獨(dú)作為陽(yáng)性對(duì)照。CD68ZM-0060ZM-0464正常腦+扁桃體+細(xì)胞膜/漿正常腦組織可作為有效的陽(yáng)性對(duì)照：小膠質(zhì)細(xì)胞必須呈現(xiàn)盡可能強(qiáng)的細(xì)胞漿染色，無(wú)背景染色。 扁桃體亦可作為陽(yáng)性對(duì)照：濾泡間巨噬細(xì)胞必須呈現(xiàn)中等至強(qiáng)的細(xì)胞漿染色，而生發(fā)中心的B細(xì)胞為陰性。如果應(yīng)用克隆號(hào)為KP1的單抗，在鱗狀上皮細(xì)胞中有弱至中等強(qiáng)度的胞漿染色是可以接受的。CD79aZA-0293扁桃體+闌尾+細(xì)胞膜扁桃體和闌尾都可以

8、作為陽(yáng)性對(duì)照：生發(fā)中心的B細(xì)胞必須呈現(xiàn)至少中等強(qiáng)度以上的明確的細(xì)胞膜染色，而平滑肌細(xì)胞陰性反應(yīng)。如果僅套區(qū)的B細(xì)胞反應(yīng)，表明該抗體不足以顯示CD79a蛋白表達(dá)比較低的腫瘤，如漿細(xì)胞瘤和前B細(xì)胞急性淋巴細(xì)胞白血病。CD117ZM-0437闌尾+細(xì)胞膜/漿闌尾為推薦的陽(yáng)性細(xì)胞：Cajal細(xì)胞必須呈現(xiàn)明確的染色，而肌層的平滑肌細(xì)胞必須是陰性的。CDX2ZA-0520胰腺+細(xì)胞核Pancreas is an appropriate control: A weak to moderate, distinct nuclear reaction in the majority of the duct epi

9、thelial cells in the pancreas must be seen.CGAZA-0066ZA-0507闌尾+細(xì)胞漿appendix is a reliable control for CGA: An at least weak to moderate distinct granular staining must seen in the axons and ganglion cells of the peripheral nerves. A strong staining must be seen in the neuroendocrine cells in the appe

10、ndiceal mucosa. A diffusion of the staining has to be accepted in the vicinity of these cells. The epithelial cells in the mucosa and the smooth muscle cells should be negative. Normal appendix is a recommendable control tissue: The normal ganglion cells and axons in the peripheral nerves must

11、show an at least weak to moderate distinct granular reaction, while the surrounding smooth muscle cells should be negative.Cyclin D1ZA-0101扁桃體+細(xì)胞核Normal tonsil is a reliable control: In the squamous epithelium, the suprabasal cells should display a strong nuclear reaction with only minimal cytoplasm

12、ic staining. In the lymphatic areas only the endothelial cells should show a nuclear reaction.Tonsil is an appropriate control for CyD1: the suprabasal layer of squamous epithelium should express strong staining while the lymphoid tissue should be unstained.CK（高分子）CK5CK5&6CK14ZA-0518ZM-0313ZA-05

13、40食管+細(xì)胞漿Esophagus is a recommendable positive control for CK-HMW in which the squamous epithelial cells must show an as strong as possible staining without background reaction. The mAb clone 34BE12 can not be recommended as a marker for CK-HMW due to an excessive cross reaction with CK-LMW in e.g. b

14、reast ductal epithelial cells giving a false positive staining reaction.CK（低分子）CK8&18ZM-0315肝臟+細(xì)胞漿liver was a reliable positive control, as all laboratories that could demonstrate the membranous reaction in the hepatocytes also could demonstrate CK-LMW in the renal cell carcinoma, which in this run