虎杖苷作用機(jī)制 - Medchemexpress - MCE中國(guó)

1、Product Data SheetPolydatinCat. No.: HY-N0120ACAS No.: 27208-80-6分式: CHO分量: 390.38作靶點(diǎn): Autophagy; Apoptosis; Mitophagy作通路: Autophagy; Apoptosis儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 29 mg/mL (74.29 mM)* means soluble, but saturation unknown.SolventMass

2、1 mg 5 mg 10 mgConcentration制備儲(chǔ)備液1 mM 2.5616 mL 12.8080 mL 25.6161 mL5 mM 0.5123 mL 2.5616 mL 5.1232 mL10 mM 0.2562 mL 1.2808 mL 2.5616 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)內(nèi)使，-20C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)內(nèi)使。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?。以下溶解?/p>

3、都請(qǐng)先按照 In Vitro 式配制澄清的儲(chǔ)備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄 的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的式助溶1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (6.40 mM); Clear solution此案可獲得 2.5 mg/mL (6.40 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取

4、100 L 25.0 mg/mL 的澄 DMSO 儲(chǔ)備液加到 400 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (6.40 mM); Clear solutionPage 1 of 2 www.MedChemE此案可獲得 2.5 mg/mL (6.40 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲(chǔ)備液加

5、到 900 L 20% 的 SBE-CD 理鹽溶液中，混合均勻。3. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (6.40 mM); Clear solution此案可獲得 2.5 mg/mL (6.40 mM，飽和度未知) 的澄 溶液，此案不適于實(shí)驗(yàn)周 期在半個(gè)以上的實(shí)驗(yàn)。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲(chǔ)備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Polydatin提取從傳統(tǒng)中藥虎杖根，在多個(gè)實(shí)驗(yàn)?zāi)Ｐ椭芯哂锌寡鬃鳌olydatin (Pi

6、ceid) 可抑制G6PD，并誘導(dǎo)氧化和內(nèi)質(zhì)應(yīng)激IC & Target NF-B, Autophagy體外研究 Polydatin protects cerebral cells from ischemic damages via improvement of microcirculation and inhibition ofplatelet aggregation. In addition, polydatin inhibits ICAM-1 expression in endothelial cells stimulated bylipopolysaccharide; it also a

7、ttenuates adhesion between white blood cells and endothelial cells1.Polydatin inhibits cancer cell proliferation and cell cycle progression4.Polydatin induces ER stress-driven cell death4.體內(nèi)研究 Polydatin could significantly increase the activity of SOD and the heart rate, attenuate myocardial patholo

8、gicaldamage, decrease MDA content, slightly increase arterial pressure and GSH-Px activity, reduce intervals of QRS, QT and ST, and lower FFA content2.PROTOCOLCell Assay 3 The effect of polydatin on mMEC viability is evaluated with an MTT assay. mMECs are incubated in the presence orabsence of vario

9、us concentrations of polydatin (25, 50 and 100 g/mL) and DEX (100 g/mL) for 24 h. Next, 20 L ofMTT (5 mg/mL) is added to each well and incubated for 4 h. After the supernatants are removed and the formazan isdissolved with 150 L of DMSO in each well, the optical density (OD) value is measured at 570

10、 nm on a microplatereader3.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Rats: Rats are divided into six groups by random assignment and treated as follows: in normal group and polydatinAdministration 23 control group: rats are administrated wi

11、th CMC-Na and polydatin (200 mol/kg) by gavage respectively, and givennormal saline (NS) by tail intravenous (iv) injection with the same volume; in DOX group: rats are injected with DOXby cauda vein for 4 weeks (3 mg/kg per week), the cumulative dosage is 12 mg/kg similar to that in the research of

12、Chang et al2.Mice: Polydatin is dissolved in dimethyl sulfoxide (DMSO) and diluted in Dulbeccos modified Eagles medium(DMEM). Sixty adult female postpartum and lactating BALB/c mice (68 weeks old, weighing 3540 g) are obtained.Control group (CG): The mice are treated with 100 L of PBS as a vehicle c

13、ontrol. Polydatin groups: 24h after S aureusinfection, the mouse model of S aureus mastitis is intraperitoneally administered polydatin at 15, 30 and 45 mg/kg3.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Page 2 of 3 www.MedChemEREFERENCES1. Cheng Y,

14、 et al. Involvement of cell adhesion molecules in polydatin protection of brain tissues from ischemia-reperfusioninjury. Brain Res. 2006 Sep19;1110(1):193-200.2. Wang HL, et al. Synergistic effects of Polydatin and Vitamin C in Inhibiting Cardiotoxicity induced by Doxorubicin in rats. Fundam Clin Ph

15、armacol. 2016Nov 28. Epub ahead of print3. Jiang KF, et al. Polydatin ameliorates Staphylococcus aureus-induced mastitis in mice via inhibiting TLR2-mediated activation of the p38 MAPK/NF-Bpathway. Acta Pharmacol Sin. 2016 Nov 28. Epub ahead of print4. Luigi Mele, et al. A New Inhibitor of glucose-6-phosphate Dehydrogenase Blocks Pentose Phosphate Pathway and Suppresses Malignant Proliferationand Metastasis in Vivo. Cell Death Dis. 2018 May