潮霉素B作用機制 - Medchemexpress - MCE中國

1、Product Data SheetHygromycin BCat. No.: HY-B0490CAS No.: 31282-04-9分式: CHNO分量: 527.52作靶點: Bacterial; Fungal作通路: Anti-infection儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 250 mg/mL (473.92 mM; Need ultrasonic)H2O : 16.67 mg/mL (31.60 mM; Need ultrasonic)Solv

2、entMass1 mg 5 mg 10 mgConcentration制備儲備液1 mM 1.8957 mL 9.4783 mL 18.9566 mL5 mM 0.3791 mL 1.8957 mL 3.7913 mL10 mM 0.1896 mL 0.9478 mL 1.8957 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。儲備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲存時，請在 6 個內(nèi)使，-20C 儲存時，請在 1 個內(nèi)使。體內(nèi)實驗請根據(jù)您的實驗動物和給藥式選擇適當?shù)娜芙獍?/p>

3、。以下溶解案都請先按照 In Vitro 式配制澄清的儲備液，再依次添加助溶劑：為保證實驗結果的可靠性，澄 的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的作液，建議您現(xiàn)現(xiàn)配，當天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的式助溶1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 6.25 mg/mL (11.85 mM); Clear solution此案可獲得 6.25 mg/mL (11.85 mM，飽和度未知) 的澄清溶液。以 1

4、mL 作液為例，取 100 L 62.5 mg/mL 的澄 DMSO 儲備液加到 400 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 6.25 mg/mL (11.85 mM); Clear solutionPage 1 of 2 www.MedChemE此案可獲得 6.25 mg/mL (11.85 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 62.5 mg/m

5、L 的澄 DMSO 儲備液加到 900 L 20% 的 SBE-CD 理鹽溶液中，混合均勻。3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 6.25 mg/mL (11.85 mM); Clear solution此案可獲得 6.25 mg/mL (11.85 mM，飽和度未知) 的澄 溶液，此案不適于實驗周 期在半個以上的實驗。以 1 mL 作液為例，取 100 L 62.5 mg/mL 的澄 DMSO 儲備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Hygromycin B抑制原核和真核細胞的氨 糖苷類抗素

6、。IC & Target Target：Antibacterial; Antifungal1體外研究 Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized byStreptomyces hygroscopicus1. Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in richmedia, presumably by preventin

7、g protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-freeextracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B.The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-d

8、ependenttranslocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNAbound to the ribosomal acceptor site2.體內(nèi)研究 Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading invivo3. Constituti

9、ve expression of the bacterial hygR gene in transgenic mice in vivo confers resistance to hygromycinB4.PROTOCOLAnimal Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated withAdministration 4 a single dose of hygromycin B i.p. at doses that star

10、ts at 2.7 mg/kg and increases by 50% for each consecutive dose.Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL.The health status and body weights of animals are monitored daily for 10 consecutive days4.MCE has not independently conf

11、irmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻 Cell Metab. 2018 Oct.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus-the producing organism. J

12、Gen Microbiol. 1985Jun;131(6):1289-98.Page 2 of 3 www.MedChemE2. Gonzlez A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec21;521(2):459-69.3. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502.McePdfHeightCaution: Product has not be