L-Homocysteine-thiolactone-hydrochloride-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEL-Homocysteine thiolactone hydrochlorideCat. No.: HY-101404ACAS No.: 31828-68-9分式: CHClNOS分量: 153.63作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) H2O : 23 mg/mL (149.71 mM)

2、* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 6.5091 mL 32.5457 mL 65.0915 mL5 mM 1.3018 mL 6.5091 mL 13.0183 mL10 mM 0.6509 mL 3.2546 mL 6.5091 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 L-Homocysteine thiolactone hydrochloride同

3、型半胱氨酸的分內(nèi)硫酯; 防同型半胱氨酸轉(zhuǎn)化成蛋質(zhì)。體外研究In all cell types, from bacterial to human, homocysteine is metabolized to homocysteine thiolactone bymethionyl-tRNA synthetase. Elevated levels of homocysteine are an independent risk factor forcardiovascular disease in humans. Homocysteine can be harmful to human cells

4、 because of its metabolicconversion to homocysteine thiolactone, a reactive thioester. This conversion occurs in all human cell types,1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEincluding endothelial cells 1. Homocysteine thiolactone induces cell death and features of apoptosisincluding increas

5、ed phosphotidylserine exposure on the membrane surface, increased apoptotic cells withhypoploid DNA contents, and internucleosomal DNA fragmentation in HL-60 cells 2. Homocysteinethiolactone is cytotoxic and capable of promoting cell death, as measured by caspase-3 activation and DNAfragmentation. H

6、cyT strongly activates IL-8 release 3.體內(nèi)研究 Homocysteine thiolactone is toxic, induces epileptic seizures in rodents, and has been implicated inAlzheimers disease 4. Homocysteine thiolactone induces two types of seizures, the coexistence ofconvulsive and nonconvulsive epilepsy. The grade of seizures

7、is dose dependent 5.PROTOCOLCell Assay 3 HUVEC are treated with homocysteine (1, 5, 10 mM) or homocysteine thiolactone (0.25, 0.5, 1 mM) for 3, 6,12 and 24 h. Cell survival is determined by the MTT method. The optical density is measured at 570 nm with630 nm as a reference 3.MCE has not independentl

8、y confirmed the accuracy of these methods. They are for reference only.Animal Rats: Homocysteine thiolactone is freshly prepared in saline and administered in a volume of 1.0 mL/100 gAdministration 5 body weight. Rats are divided into control (saline-injected) group and homocysteine thiolactone-trea

9、tedgroup. The latter group receives different doses 5.5 mM/kg, 8.0 mM/kg, and 11.0 mM/kg, respectively. Eachrat is used only once 5.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Jakubowski H, et al. Homocysteine thiolactone: metabolic ori

10、gin and protein homocysteinylation in humans. J Nutr. 2000 Feb;130(2SSuppl):377S-381S.2. Huang RF, et al. Homocysteine thiolactone induces apoptotic DNA damage mediated by increased intracellularhydrogen peroxide andcaspase 3 activation in HL-60 cells. Life Sci. 2001 May 11;68(25):2799-811.3. Kerken

11、i M, et al. Comparative study on in vitro effects of homocysteine thiolactone and homocysteine on HUVECcells: evidence for astronger proapoptotic and proinflammative homocysteine thiolactone. Mol Cell Biochem. 2006 Oct;291(1-2):119-26.4. Borowczyk K, et al. Metabolism and neurotoxicity of homocystei

12、ne thiolactone in mice: evidence for a protective role of paraoxonase 1. JAlzheimers Dis. 2012;30(2):225-31.5. Stanojlovi O, et al. Two types of seizures in homocysteine thiolactone-treated adult rats, behavioral and electroencephalographic study.Cell Mol Neurobiol. 2009 May;29(3):329-39.McePdfHeightCaution: P