Sodium-Butyrate-Butanoic-acid-sodium-salt-DataSheet-生命科學試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESodium ButyrateCat. No.: HY-B0350ACAS No.: 156-54-7Synonyms: Butanoic acid sodium salt分式: CHNaO分量: 110.09作靶點: HDAC; Autophagy作通路: Cell Cycle/DNA Damage; Epigenetics; Autophagy儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 m

2、onths-20C 1 month溶解性數(shù)據(jù)體外實驗 H2O : 100 mg/mL (908.35 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 9.0835 mL 45.4174 mL 90.8348 mL5 mM 1.8167 mL 9.0835 mL 18.1670 mL10 mM 0.9083 mL 4.5417 mL 9.0835 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內(nèi)實驗 Sodiu

3、m butyrate (SB) is prepared in PBS5.BIOLOGICAL ACTIVITY物活性 Sodium Butyrate (Butanoic acid sodium salt)種短鏈脂 酸，可以抑制組蛋去酰化酶。IC50 & Target HDAC1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE體外研究 Sodium Butyrate induces morphological changes, inhibits cell proliferation and impairs cell viability in NPC

4、cells. Sodium Butyrate (1, 5, 10 mM) is cytotoxic to NPC cells, inducing a dose- and time-dependentdecrease in cell viability, in both 5-8F and 6-10B cells. Sodium Butyrate induces nasopharyngeal carcinomacell apoptosis by activating the mitochondrial apoptotic axis. Moreover, SOCE inhibition and di

5、sruption of theCRAC channel can attenuate the apoptosis induced by Sodium Butyrate 1. Sodium butyrate significantlydecreases cell viability, accompanied by reduced levels of p-mTOR and PCNA protein. Sodium butyrate, in adose-dependent manner, induces cell cycle arrest in G0/G1 phase and reduces the

6、numbers of cells in Sphase. In addition, relative expression of p21, p27, and pro-apoptosis bak genes, and protein levels ofp21Waf1/Cip1, p27Kip1, cyclinD3, CDK4, and Cleave-caspase3 are increased by higher concentrations ofsodium butyrate (1, 5, 10 mM), and the levels of cyclinD1 and CDK6 are reduc

7、ed by 5 and 10 mM butyrate3.體內(nèi)研究 Sodium Butyrate (300 mg/kg, s.c.) administration immediately after HI provided almost completeneuroprotection in comparison with non-treated animals. Sodium butyrate administration results in anincreased number of microglial cells to 150% of vehicle-treated animals i

8、n the ipsilateral side. Sodiumbutyrate promotes the polarization of microglia from M1- to M2-like phenotype after neonatal hypoxia-ischemia 2. Sodium butyrate (300 mg/kg, s.c.) in combination with AK-7 (20 mg/kg, i.p.) significantlyalleviates this reduction of the time spent exploring new objects, a

9、meliorates the reduction of the number ofKi67-positive cells and DCX-immunoreactive neuroblasts in the dentate gyrus of the mice. In addition,sodium butyrate reverses SIRT2-related age phenotypes 4.PROTOCOLCell Assay 1 Cells are seeded at a density of 2,000 cells/well in 96-well plates with 200 L cu

10、lture medium containingSodium Butyrate at different concentrations. Then, the cells are consecutively cultured for 72 h. Every 24 h,20 L 5 mg/mL MTT solution is added into the corresponding well, and the cells are cultured for another 4 h.Then, the solution is replaced with 150 L dimethylsulfoxide,

11、followed by gentle agitation of the plates for 15min at room temperature. Finally, the absorbance at 492 nm is measured to represent the cell viability.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Seven-day-old rat pups are subjected to unilat

12、eral carotid artery ligation followed by 60 min of hypoxia (7.6%Administration 2 O2). Sodium Butyrate (300 mg/kg) is administered in a 5-day regime with the first injection given immediatelyafter hypoxic exposure. The damage of the ipsilateral hemisphere is evaluated by hematoxylin-eosin staining6 d

13、ays after the insult. Samples are collected at 24 and 48 h and 6 days.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻 Neurobiol Dis. 2017 Dec 14;111:12-25. BMC Vet Res. 2019 Jul 29;15(1):267. Patent. US20180263995A1.See more customer validat

14、ions on HYPERLINK / www.MedChemE2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemEREFERENCES1. Huang W, et al. Inhibition of store-operated Ca2+ entry counteracts the apoptosis of nasopharyngeal carcinoma cells induced by sodiumbutyrate. Oncol Lett. 2017 Feb;13(2):921-9292. Jaworska J, et al. The pot

15、ential neuroprotective role of a histone deacetylase inhibitor, sodium butyrate, after neonatal hypoxia-ischemia.J Neuroinflammation. 2017 Feb 10;14(1):343. Qiu Y, et al. Effect of sodium butyrate on cell proliferation and cell cycle in porcine intestinal epithelial (IPEC-J2) cells. In Vitro Cell De

16、vBiol Anim. 2017 Jan 274. Jung HY, et al. Sirtuin-2 inhibition affects hippocampal functions and sodium butyrate ameliorates the reduction in novel object memory,cell proliferation, and neuroblast differentiation. Lab Anim Res. 2016 Dec;32(4):224-2305. Wang P, et al. Sodium butyrate triggers a functional elongation of microglial process via Akt-small RhoGTPase activation and HDACsinhi