布魯菌屬-微生物教學(xué)課件

1、Medical MicrobiologyDepartment of Microbiology, HMU第二篇 致病性細(xì)菌第24章布魯菌屬Brucella 微生物學(xué)教研室 鐘照華教授 商慶龍講師 教學(xué)大綱掌握內(nèi)容掌握主要生物學(xué)性狀（抗原構(gòu)造與分型） 熟悉內(nèi)容熟悉布魯菌的免疫性及微生物學(xué)檢查法 了解內(nèi)容了解布魯菌的防治原則 問題布魯菌抗原構(gòu)造與分型？布魯菌是如何致病的？布魯菌引起的免疫反應(yīng)如何？ 布魯菌屬 Brucella引起人類、家畜和其它動(dòng)物布魯菌病分6個(gè)生物種羊布魯菌 B. melitensis牛布魯菌 B. abortus豬布魯菌 B. suis鼠布魯菌 B. meotomae綿羊布魯菌

2、B. ovis犬布魯菌 B. canis生物學(xué)性狀革蘭陰性小桿菌無鞭毛，不形成芽胞，光滑型菌株有微莢膜 專性需氧生長緩慢分解葡萄糖，產(chǎn)生少量酸過氧化氫酶陽性，氧化酶陽性產(chǎn)生H2SB. melitensis分泌物、乳品消化道、呼吸道粘膜、皮膚及眼結(jié)膜人菌血癥16W潛伏期（70）發(fā)熱、出汗、關(guān)節(jié)痛和全身乏力內(nèi)毒素進(jìn)入肝、脾、骨髓（發(fā)熱消退）繁殖入血菌血癥（體溫再次上升）波浪熱致病性致病物質(zhì)內(nèi)毒素、透明質(zhì)酸酶、莢膜所致疾病母畜流產(chǎn)赤蘚醇胎盤、絨毛膜含量高Brucella species SummaryBiologySmall gram-negative rods without capsuleObl

3、igatory aerobicGrows slowly in enriched culture media with 10% CO2H2S + Pathogens Human pathogen Animal reservoirsBrucella melitensis goat, sheepBrucella abortus cattleBrucella suis pigTransmissionEnter human body by Ingestion of contaminated milk productsThrough the skin directly contact in an occu

4、pational setting such as abattoir Disease: BrucellosisAnimalAbortionHumanWeakness and fatigueEnlarged lymph nodes, liver, and spleenUndulant fever (rising-and-falling fever)Occurs in a few patientsDiagnosisIsolationSample: blood, bone marrowSerological examinationAgglutination testComplement fixation testCoombs test Skin testPrevention & TherapyPasteurization of milkImmunizationAnimalsOccupational high-risk personsSlaug