Trolox-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemETroloxCat.No.:HY-101445CASNo.:53188-07-1分?式:C??H??O?分?量:250.29作?靶點(diǎn):ReactiveOxygenSpecies;Ferroptosis;Apoptosis作?通路:Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB;Apoptosis儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:≥100mg/mL(399.54mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.9954mL19.9768mL39.9537mL5mM0.7991mL3.9954mL7.9907mL10mM0.3995mL1.9977mL3.9954mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請?jiān)?個?內(nèi)使?，-20°C儲存時，請?jiān)?個?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(8.31mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(8.31mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(8.31mM);ClearsolutionBIOLOGICALACTIVITY?物活性Trolox?種維?素E的類似物，具有強(qiáng)?的抗氧化作?。Trolox還?種強(qiáng)?的膜損傷抑制劑。體外研究Troloxhasshowntoprotectmammaliancellsfromoxidativedamage.Troloxiseffectiveinpreventingmyocytenecrosisincellculturestudiesandinacaninemodeloftwohoursofleftanteriordescendingcoronaryartery(LAD)occlusionfollowedbyfourhoursofreperfusion[1].Troloxcouldpreventoxidativestress-inducedapoptosisinthymocytes.Pre-orposttreatmentofcellswithTroloxreducedH2O2-inducedDNAfragmentationtocontrollevelsandbelow.[2].Troloxisahydrophilicanalogueofalpha-tocopherolandreportedtoscavengeperoxylradicalsbetterthanvitaminEinsodiumdodecylsulfatemicellesandinliposomes.Troloxprolongssubstantiallythesurvivalofhumanventricularmyocytesandhepatocyteagainstoxyradicalsgeneratedwithxanthineoxidaseplushypoxanthine,andpreventedlysisofredcellsexposedtoanazo-initiator[3].PROTOCOLCellAssay[1]ThymocytesaretreatedwithTrolox(10mM)before,during,orafter10minexposureto10μMH2O2.TroloxincubationsthatareconductedbeforeorafterH2O2exposuresareperformedinTCM.TroloxtreatmentsthatareadministeredconcurrentlywithH2O2areperformedinPBSwithferroussulfate[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?CellRep.2022Oct11;41(2):111462.?CellRep.2022Sep20;40(12):111381.?CellDeathDis.2021Apr1;12(4):338.?OxidMedCellLongev.2020Jan27;2020:5829521.?CellDeathDiscov.2022May2;8(1):238.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].MickleDA,etal.Myocardialsalvagewithtroloxandascorbicacidforanacuteevolvinginfarction.AnnThoracSurg.1989Apr;47(4):553-2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE7.[2].ForrestVJ,etal.Oxidativestress-inducedapoptosispreventedbyTrolox.FreeRadicBiolMed.1994Jun;16(6):675-84.[3].WuTW,etal.ThecytoprotectiveeffectofTroloxdemonstratedwiththreetypesofhumancells.BiochemCellBiol.1990Oct;68(10):1189-94.McePdfHeightCaution:Producthasnotbeen