Brefeldin-A-BFA-DataSheet-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEBrefeldinACat.No.:HY-16592CASNo.:20350-15-6Synonyms:BFA;Cyanein;Decumbin分?式:C??H??O?分?量:280.36作?靶點:Autophagy;CRISPR/Cas9;Mitophagy;HSV;Antibiotic;Bacterial作?通路:Autophagy;CellCycle/DNADamage;Anti-infection儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:100mg/mL(356.68mM;Needultrasonic)Ethanol:11.11mg/mL(39.63mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.5668mL17.8342mL35.6684mL5mM0.7134mL3.5668mL7.1337mL10mM0.3567mL1.7834mL3.5668mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內使?，-20°C儲存時，請在1個?內使?。體內實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(8.92mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(8.92mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(8.92mM);Clearsolution4.請依序添加每種溶劑：10%EtOH>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(8.92mM);Clearsolution5.請依序添加每種溶劑：10%EtOH>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(8.92mM);Clearsolution6.請依序添加每種溶劑：10%EtOH>>90%cornoilSolubility:≥2.5mg/mL(8.92mM);ClearsolutionBIOLOGICALACTIVITY?物活性BrefeldinA(BFA)?種內酯抗?素，蛋?質運輸?shù)奶囟ㄒ种苿?。BrefeldinA阻斷分泌蛋?和膜蛋?從內質?向?爾體的轉運。BrefeldinA也?種?噬(autophagy)和mitophagy抑制劑。BrefeldinA還?種CRISPR/Cas9激動劑，可抑制HSV-1病毒，并具有抗癌活性。IC50&TargetHSV-1CRISPR/Cas9HSV-1體外研究BrefeldinA(BFA)treatmentfor15hor40h,causesdramaticswellingoftheEndoplasmicReticulum(ER)andshiftsitslocalizationtotheperipheryofnormalratkidney(NRK)cells.ProlongedBrefeldinAtreatmentresultsinmarkeddisruptionoftheMTandactincytoskeleton[1].ADP-ribosylationofBARSismediatedbyformationofaconjugatebetweenBrefeldinAandADPR.BARSshowsBACbindingwhenincubatedwiththemediumfromtheBFA-treatedCD38+HeLacells[3].BrefeldinAinducesanchorage-independentcelldeathinMDA-MB-231breastcancercells,inhibitstheformationofMDA-MB-231coloniesin3Dand2DculturesandinhibitsthemigrationandMMP9(MatrixMetallopeptidase9)activityofMDA-MB-231[2].PROTOCOLCellAssay[1]Cellsaregrownonglasscoverslips,fixedin3%paraformaldehydeinPBS(10minatroomtemperature)andthenwashedinPBS.Cellsarepermeabilizedwith0.01%TritonX-lOOinPBSatroomtemperaturefor7min.Thecoverslipsarewashed(3timesinPBS/0.2%Tween)incubatedinPBS/O.4%fishskingelatin/0.2%Tween(5min)andinPBS/2.5%goatserum/0.2%Tween(5min.).Afterblocking,thecellsareincubatedwithprimaryantibodiesfor45minat37°C,andthenwashedwithPBS/0.2%Tween(5times,5mineach).Thesecondaryantibodiesareaddedfor30minat37°Candthencellsarewashedasabove.Coverslipsaremountedonslidesin9:1glycerol/PBSwith0.1%o-phenylenediamine.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE戶使?本產(chǎn)品發(fā)表的科研?獻?NatMicrobiol.2022Sep;7(9):1361-1375.?JAutoimmun.2019May;99:39-47.?CancerRes.2020Sep15;80(18):3892-3905.?JImmunotherCancer.2022Dec;10(12):e005651.?Theranostics.2021Jun1;11(15):7491-7506.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].AlvarezC,etal.BrefeldinA(BFA)disruptstheorganizationofthemicrotubuleandtheactincytoskeletons.EurJCellBiol.1999Jan;78(1):1-14.[2].ColanziA,etal.MolecularmechanismandfunctionalroleofbrefeldinA-mediatedADP-ribosylationofCtBP1/BARS.ProcNatlAcadSciUSA.2013Jun11;110(24):9794-9.[3].TsengCN,etal.BrefeldinAreducesanchorage-independentsurvival,cancerstemcellpotentialandmigrationofMDA-MB-231humanbreastcancercells.Molecules.2014Oct29;19(11):17464-77.[4].WangJ,etal.Erythroleukemiacellsacquireanalternativemitophagycapability.SciRep.2016Apr19;6:24641.[5].YuC,etal.SmallmoleculesenhanceCRISPRgenomeeditinginpluripotentstemcells.CellStemCell.2015Feb5;16(2):142-7.[6].NozawaN,etal.Subcellularlocalizationofherpessimplexvirustype1UL51proteinandroleofpalmitoylationinGolgiapparatustargeting.JVirol.2003Mar;77(5):3204-16.[7].JensenHL,RygaardJ,NorrildB.Atime-relatedstudyofBrefeldinAeffectsinHSV-1infectedculturedhumanfibroblasts.APMIS.1995;103(7-8):530-539.doi:1