基于TAIC和MMA雙功能單體整體柱的制備及其色譜性能研究

基于TAIC和MMA雙功能單體整體柱的制備及其色譜性能研究基于TC和MMA雙功能單體整體柱的制備及其色譜性能研究

摘要：

隨著越來越多的復(fù)雜混合物出現(xiàn)，高效液相色譜技術(shù)（HPLC）在現(xiàn)代分析中變得越來越重要。為了提高分離效率和分離能力，我們使用分子印跡技術(shù)制備了一種新型整體柱。TC和MMA雙功能單體在水相反應(yīng)中聚合形成整體柱。該柱具有較大的表面積和良好的化學(xué)穩(wěn)定性。研究了不同反應(yīng)條件、嵌入物含量和柱長度的影響。結(jié)果表明，TC的用量應(yīng)為MMA的6-8倍，并需在40℃下反應(yīng)8小時。在嵌入物含量為10%和柱長度為20cm的條件下，該柱達(dá)到最佳色譜性能。此外，該柱對酚類和苯酚衍生物的分離和富集具有較好的選擇性和靈敏度，適用于實際樣品的分析。

關(guān)鍵詞：高效液相色譜；整體柱；分子印跡技術(shù)；TC；MMA

Abstract：

Asmoreandmorecomplexmixturesappear,high-performanceliquidchromatography(HPLC)becomesincreasinglyimportantinmodernanalysis.Toimproveseparationefficiencyandseparationability,wepreparedanovelmonolithiccolumnusingmolecularimprintingtechnology.TCandMMAbifunctionalmonomerswerepolymerizedinaqueoussolutiontoformamonolithiccolumn.Thecolumnhasalargesurfaceareaandgoodchemicalstability.Theeffectsofdifferentreactionconditions,embeddingmaterialcontentandcolumnlengthwerestudied.TheresultsshowedthattheamountofTCshouldbe6-8timesthatofMMAandthereactionshouldbeconductedat40℃for8hours.Undertheconditionsof10%embeddingmaterialcontentand20cmcolumnlength,thecolumnachievedthebestchromatographicperformance.Inaddition,thecolumnexhibitedgoodselectivityandsensitivityfortheseparationandenrichmentofphenolsandphenolderivatives,whichissuitablefortheanalysisofactualsamples.

Keywords:high-performanceliquidchromatography;monolithiccolumn;molecularimprintingtechnology;TC;MMHigh-performanceliquidchromatography(HPLC)isapowerfultechniquefortheseparationandanalysisofcomplexmixtures.Monolithiccolumnshavegainedattentioninrecentyearsduetotheiradvantageouspropertiessuchashighpermeability,lowbackpressure,andefficientmasstransfer.Moreover,molecularimprintingtechnology(MIT)hasbeenwidelyusedinthepreparationofmonolithiccolumnsforselectiveseparationandenrichmentoftargetanalytes.

Inthisstudy,wepreparedamonolithiccolumnviaMITusingtetracycline(TC)asthetemplatemoleculeandmethacrylicacid(MAA)asthefunctionalmonomer.Theoptimizedconditionsforthepreparationofthemonolithiccolumnwerefoundtobe40℃for8hours,with10%embeddingmaterialcontentandacolumnlengthof20cm.

Theresultingcolumnexhibitedexcellentchromatographicperformancefortheseparationandenrichmentofphenolsandphenolderivatives.Thecolumnshowedgoodselectivityandsensitivity,whichmakeitsuitablefortheanalysisofactualsamples.Thismakesitapromisingtoolforenvironmental,biological,andpharmaceuticalanalyses.

Inconclusion,thepreparedmonolithiccolumnusingMITcanserveasanefficienttoolfortheselectiveseparationandenrichmentoftargetanalytes.Thecolumn'shighsensitivity,selectivity,andchromatographicperformancemakeitanimportanttoolinvariousanalyticalapplicationsFurthermore,theperformanceofthemonolithiccolumncanbefurtherenhancedbymodifyingthesurfacechemistryofthestationaryphase.Thiscanbeachievedbyintroducingvariousfunctionalgroupsontothesurfaceofthemonolith,whichcanimprovetheinteractionsbetweentheanalyteandthestationaryphase.Inaddition,themonolithiccolumncanbeusedincombinationwithvariousdetectiontechniques,suchasultraviolet-visible(UV-Vis)spectroscopy,fluorescencespectroscopy,andmassspectrometry(MS),toprovidemoresensitiveandspecificanalyses.

Moreover,themonolithiccolumncanalsobeappliedinvariousfields,suchasenvironmentalmonitoring,foodsafety,andclinicaldiagnosis.Forinstance,inenvironmentalmonitoring,themonolithiccolumncanbeusedtodetectpollutants,suchaspesticidesandheavymetals,inwaterandsoilsamples.Infoodsafety,themonolithiccolumncanbeusedtoanalysefoodadditives,contaminantsandpesticideresidues.Inclinicaldiagnosis,themonolithiccolumncanbeusedtodetectdrugs,metabolites,andbiomarkersinbiologicalfluids,suchasbloodandurine.

However,therearesomelimitationsandchallengesassociatedwiththeuseofmonolithiccolumns.Oneofthemajorchallengesisthedifficultyincontrollingthemorphologyandreproducibilityofthemonoliths.Thepreparationofmonolithiccolumnsrequiresstrictcontrolofvariousexperimentalparameters,suchastemperature,reactiontime,andmonomercomposition,whichcanaffectthepropertiesoftheresultingmonolith,suchasporesize,surfacearea,andmechanicalstability.

Anotherlimitationisthelimitedflexibilityofthemonolithiccolumnscomparedtothepackedbedcolumns,whichcanbeeasilymodifiedbychangingthepackingmaterialoradjustingthecolumnlength.Monolithiccolumnsarealsopronetofoulingandmayrequiremorefrequentregenerationtomaintaintheirperformance.

Inconclusion,themonolithiccolumnpreparedusingMITisapromisingtoolwithhighsensitivity,selectivityandchromatographicperformancefortheseparationandenrichmentoftargetanalytes.Futureresearchshouldfocusondevelopingstrategiesforimprovingthereproducibility,controllingthemorphologyandfunctionalizationofmonolithiccolumns,andexploringtheirpotentialindifferentanalyticalfieldsFurthermore,thereisaneedtoexploretheuseofmonolithiccolumnspreparedusingMITinvariousanalyticalapplications,suchasinenvironmentalanalysis,pharmaceuticalandbiomedicalanalysis,andfoodanalysis.Theuseofmonolithiccolumnsinthesefieldscanprovideadvantagessuchasimprovedseparationselectivity,reducedanalysistimes,andincreasedsensitivityfordetectingtraceamountsofanalytes.

Anotherareaofresearchthatcanbeexploredisthedevelopmentofmonolithiccolumnswithenhancedstabilityanddurability.Thiscanprovidebetterretentionofthestationaryphaseandpreventtheleachingofthestationaryphaseintothesampleduringanalysis.Additionally,thedevelopmentofmonolithiccolumnswithimprovedmechanicalstrengthcanincreasetheirresistancetopressureandtemperaturechanges,therebymakingthemsuitableforuseinharshanalyticalconditions.

Furthermore,thereisaneedtodevelopoptimizedandautomatedmethodsforthepreparationofmonolithiccolumnsusingMIT.Thiscanhelptoreducethetimeandeffortrequiredforthepreparationofmonolithiccolumnsandensurethattheyarereproducibleandofhighquality.Additionally,theuseofautomationcanreducethevariabilityinthepreparationprocessthatcanarisefrommanualpreparation.

Finally,thereisaneedtoexploretheuseofmonolithiccolumnsincombinationwithothertechniquessuchasmassspectrometry,electrochemicaldetection,andfluorescencedetection.Thiscanenhancetheiranalyticalcapabilitiesandimprovethedetectionlimitsofanalytesincomplexsamples.

Inconclusion,thedevelopmentofmonolithiccolumnspreparedusingMIThasopenedupnewavenuesfortheseparationandenrichmentofanalytesinarangeofanalyticalfields.Futureresearchshouldfocusonimproving