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限制性內(nèi)切酶酶切位點(diǎn)匯AatII識(shí)別位點(diǎn)5;,.GACGffc,,-3^3J.CJGCAG.5JAcc65I識(shí)別位點(diǎn)5:..GT'MKAC...3*3..CAKMJG.AccI識(shí)別位點(diǎn)5...GT'MKAC...3*3..CAKMJG.5JAciI識(shí)別位點(diǎn)5rf...CfCGC...3r3\..GGCp..5JAclI識(shí)別位點(diǎn)A”CGTT..?$3.TTGC/A..5^AcuI識(shí)別位點(diǎn)5:..CTGA ;3:,GACTTC{N)1Jm3AfeI識(shí)別位點(diǎn)5J...AGC,GCT...3r3J... GA..5'AflII識(shí)別位點(diǎn)5-...cTtT^AG,,,3'3..GAATl^C.5^AflIII識(shí)別位點(diǎn)5:../TcRYGI...3J壬TGYR笛…5一AgeI識(shí)別位點(diǎn)必,足CGGUT3"TGGCC/i..5^AhdI識(shí)別位點(diǎn)5;,,GACNNhTNNGTG3,._CTGN吐NNNCAG5-AleI識(shí)別位點(diǎn)5-...CACrirrHNGTG...3r3'..GTGM^NNCAC,..5JAluI識(shí)別位點(diǎn)5:..AG*CT...3r3" —5AlwI識(shí)別位點(diǎn)5"_GGATC(N)「..丁3"...CCTAlwNI識(shí)別位點(diǎn)5「rCAGNNNpTG,,,3'3J...Gia^NMNGAC...5JApaI識(shí)別位點(diǎn)5"GGGCit…》3;.CpCGGG..5^ApaLI識(shí)別位點(diǎn)5;MTGCAC,,33:.GAGGING.ApeKI識(shí)別位點(diǎn)5;MCWGG…了3"CGWqG…5’ApoI識(shí)別位點(diǎn)5J.fTaATTY...3*3"YTTAA『一5’AscI識(shí)別位點(diǎn)5…GGlGCGg..丁3J...GCGCGCqG…5’AseI識(shí)別位點(diǎn)5:,,A「T冉AT...3"3J.TAATJA5JAsiSI識(shí)別位點(diǎn)^...GCQAfCGC...3r.CGCJaGCG..5^AvaI識(shí)別位點(diǎn)5",C*CGRG…丁3;..GRGC^C.,.5^AvaII識(shí)別位點(diǎn)5…GfcWGC…丁3J..CCWGfl.,.5JAvrII識(shí)別位點(diǎn)5P,,CGTAGG…丁MGGATCQ…5’BaeI識(shí)別位點(diǎn)5:上㈣A5NLG丁AVCW)],3-3:g(N)TG(NLCATRGj(N)”5「BamHI識(shí)別位點(diǎn)&…灸ATM…丁3.CGIAG^..BanI識(shí)別位點(diǎn),<?GYRCC...3J3""FlY冬G…寸BanII識(shí)別位點(diǎn)",GRGG『t…w3;.^YCGRG..5^BbsI識(shí)別位點(diǎn)5:..GAAGACCN)fT...T3:..CT1CT$sBbvCI識(shí)別位點(diǎn)5\..CCTtCAGC...3*3;GGAGT4CG一一亍BbvI識(shí)別位點(diǎn)5"GCA.G€(呵一33"CGTCG(M)"…&BccI識(shí)別位點(diǎn)5;..C-GATCtN)/...3一3:..GGTAQ倒矽3BceAI識(shí)別位點(diǎn)5P..ACGGCtN)/.3^3.—TGCCG(hlh喘…亍BcgI識(shí)別位點(diǎn)5-...^(NJCGAtN^TGC<N)7.站一履叫GCT㈣/CG(N&.SBciVI識(shí)別位點(diǎn)5;..GTATCC^}/...T3:.CATAGG...5^BclI識(shí)別位點(diǎn)5\..ffeATCA...3'3"ACTAGJ…5’BfaI識(shí)別位點(diǎn)5\..C?TAG...3r3".GAT^C...5JBfuAI識(shí)別位點(diǎn)..ACCTGC(N)J...3^3一一TGGRCW虹一5「BglI識(shí)別位點(diǎn)5r...GCCNNNrfNGGC..3r3J..CGGM^NNNNGCO,.,5J,BglII識(shí)別位點(diǎn)廠…心ATCT_33一一一TCT片留一5’BlpI識(shí)別位點(diǎn)5:.Gt/TNAGC-.3*3"一CGANLGG一亨Bme1580I識(shí)別位點(diǎn)5:.CKGCM^C..3^3"WCGK6,一5’BmgBI識(shí)別位點(diǎn)S」.C;A說(shuō)TC,.-3「3...GTG^pAG..,5JBmrI識(shí)別位點(diǎn)5:..ACTGGG(N)bT...y3:.TGACCC(N)^...5RBmtI識(shí)別位點(diǎn)5",GCTAG*C…3-3J.qpATCG一一5'BpmI識(shí)別位點(diǎn)5P.r.CTGGAG(M)i;T...3^3".L.GACCT..5RBpu10I識(shí)別位點(diǎn)5"GCVhlAGC,,,3r3..GGANT&CG一一5’BpuEI識(shí)別位點(diǎn)5'...CTTGAG(N)/...3*3:..GAACTC(N)f^..BsaAI識(shí)別位點(diǎn)5'一、,YAC'GTR…3-3J..RTG^CAY.,.5^BsaBI識(shí)別位點(diǎn)5P.GATNATQ.T33J.^CTANhl^NNTAC...5^BsaHI識(shí)別位點(diǎn)5…GPftGYC,9"KYG%R6…5'BsaI識(shí)別位點(diǎn)5P.r.GGTCTCCN)/...T3.L.CCAGAG(N)^...5RBsaJI識(shí)別位點(diǎn)5rf...tfCNMGG..3r3J...GGNWCj:.5^BsaWI識(shí)別位點(diǎn)5.,CGG恂…,3"3J.WGGCC//BsaXI識(shí)別位點(diǎn)5'.一二四ACCNJ^CTCC^;...33’…玨仰)■.GAGQ(M)^…5'5;.GAGGAG(N),J.T3:..CTCCTC(N) ..5^BseYI識(shí)別位點(diǎn)"0饑心?!?"3"GGGTQp…5JBsgI識(shí)別位點(diǎn)5:..GTGCAG(M}t*33,_CKCGT SBsiEI識(shí)別位點(diǎn)礦-GGRWfcG*3".GC/RGC,..5‘BsiHKAI識(shí)別位點(diǎn)5:,.GWGCWt...33J..(^WCGWG.5JBsiWI識(shí)別位點(diǎn)TACG,,.3^3J...GCATG^C..5^BslI識(shí)別位點(diǎn)5:..CCNNNhFvTiMNGG...3^3*■GGNNiNNNMNCG.,-5*BsmAI識(shí)別位點(diǎn)5".GTCTW—33-...CAGAG(M)%.一寸BsmBI識(shí)別位點(diǎn)5:..CGTCTC(N)|T..y3:..GCAGAG(N)^...5^BsmFI識(shí)別位點(diǎn)馱一GGGAC(N).cT.3*3",CCCTGfNhu,.5「BsmI識(shí)別位點(diǎn)5',,,GAATQCNTr.3^眨CTTA*N…5’BsoBI識(shí)別位點(diǎn)5;,一WCGRG-.丁3"GRGC瞥…5’Bsp1286I識(shí)別位點(diǎn)5"&時(shí)。時(shí),3.CiHCGDG..5rBspCNI識(shí)別位點(diǎn)5.CTCAG(N)11;...3J 5\3P.GAQTC(W*.5 3;BspDI識(shí)別位點(diǎn)51,■ATTCGAT,,,3^3"TAGC.TA…5’BspEI識(shí)別位點(diǎn).T,CCGGk,.3"3J.AGGCCJ..5J5.,,TcATGA,,.3r3"AGTACJ…5’BspMI識(shí)別位點(diǎn)5:.ACCTGC(N)/.T3.TGGACG(N)^...5^BspQI識(shí)別位點(diǎn)5\.GCTCTTC(U}7..3j3"CGAGAAG倒y,5'BsrBI識(shí)別位點(diǎn)ccgEc…件3".GgGAG…5’BsrDI識(shí)別位點(diǎn)51,,GCRATGNN"》3".GGrTAC^NN…5’BsrFI識(shí)別位點(diǎn)5",RP$GGV,.3「3..YGGC<^R-5JBsrGI識(shí)別位點(diǎn)5.ftTACA-3^3:..ACATGJ…5'BsrI識(shí)別位點(diǎn)5;ACTGGN^..3^3"TGA冬hl.SBssHII識(shí)別位點(diǎn)E,口CGGGC2,3J...CGCGC^G..5^BssKI識(shí)別位點(diǎn)5;,TCCNGG,.-3r3"GGNCq.SBssSI識(shí)別位點(diǎn)5…辦。GAG…3-3:.,GT^CT;C…5’BstAPI識(shí)別位點(diǎn)51一GCANNnBnTGC,,$3:.CGTN^NNNNACG...5RBstBI識(shí)別位點(diǎn),Tl'tGAA.-.3^3J..AAGCJT...5JBstEII識(shí)別位點(diǎn)CT礦MGTNACC…#GA3\.GCAhlrqG...5^BstNI識(shí)別位點(diǎn)5"C血GG.W3..GGV^CC..5"BstUI識(shí)別位點(diǎn)5;.,GG饑,.河3;..■GCJSC...5^5".tATG5".tATG…3’3"GTA%一5’FauI識(shí)別位點(diǎn)5J.CCCGC.3*3",GGGCG(N妃…5,Fnu4HI識(shí)別位點(diǎn)5;.,GC^NGC...3^,CGNpG.5^FokI識(shí)別位點(diǎn)5~一一一(iGATG(hl).T..X3...CCT _¥FseI識(shí)別位點(diǎn)5\..ggccggtcc.,.yy,—CqpGGCGGGFspI識(shí)別位點(diǎn)5;.,TG<TgGA,,,3r3"AC鳥"T…5’HaeII識(shí)別位點(diǎn)5:..rgcg<7y...y3:..^CGCGR...5JHaeIIIHgaI識(shí)別位點(diǎn)..GACGCW一一3"CTGCG{M)10..5^HhaI識(shí)別位點(diǎn)5\..GCG¥C...3rC^GCG.,.5JHincII識(shí)別位點(diǎn)礦..gt3..■GARtYTG...5JHindIII識(shí)別位點(diǎn)5;.,a7GCTT,..3r/一TTCGA^A..5JHinfI識(shí)別位點(diǎn)5...GTiNJC...3*3..CTMAJ3..5^HinP1I識(shí)別位點(diǎn)5,.,爆CGC…3"3:.-GGCJ3.,.5JHpaI識(shí)別位點(diǎn)5,riQTT>AG…事3J...CAAJTG..5^HpaII識(shí)別位點(diǎn)5:.,CTCGG,..3*3J.-GGC^C..5JHphI識(shí)別位點(diǎn)5...GGTGA_.3-3’…GC尚CT(N)?_S5;,.CCANNNNN^TGG..,3-3J.GGTNaNNNNNACC,5BstYI識(shí)別位點(diǎn)slfTgate,.YCTAG^R…5’BstZ17I識(shí)別位點(diǎn)5;,-GT^TAC-3^3:..CAT/TG…5’Bsu36I識(shí)別位點(diǎn)Sr...C(flNAGG...3r3...GGaNT4CC.5^BtgI識(shí)別位點(diǎn)5:..(fCRVGG...3*3GGYRC—一5’BtgZI識(shí)別位點(diǎn)5..GCGATG(N}/3^3-....CGCTACCN}^..5^BtsCI識(shí)別位點(diǎn)5"GCAGTG+3"CG¥CAC/IN,.5BtsI識(shí)別位點(diǎn)5"GCAGTGNN"+3.,.CGTCAC^NN...5^Cac8I識(shí)別位點(diǎn)5;,.GCffNGC…33..-CG^NCG.5JClaI識(shí)別位點(diǎn)5"ATPGAT…壬r...TAGCJA.5*CspCI識(shí)別位點(diǎn)5:.「mJN)eAN),GTGG側(cè))西】..33:..jS,13(N)GTT(N)JCACCWl(>lk....5CviAII識(shí)別位點(diǎn)5.r.C%TQ,"3"..GTA^C..5CviKI-1識(shí)別位點(diǎn)&"RG¥V…3“3J...YC^Fi...5JCviQI識(shí)別位點(diǎn)5""TAG…W3.CATJ3.,r5PDdeI識(shí)別位點(diǎn)5-...drTHAG...3r站.代ANT£一5’DpnI識(shí)別位點(diǎn)
5"GATC33"CTAG5“DpnII識(shí)別位點(diǎn)5J.^GATC...^3"一GTAG.…5'DraI識(shí)別位點(diǎn)5;,.TTAA,.,3^3".AAAJTT…5’DraIII識(shí)別位點(diǎn)5:,.CACNNNtGTG,-,3*3J.GTCyjNNCAC5JDrdI識(shí)別位點(diǎn)5"GACNNN亦MGTC…3”3;..CTGNN^NNNNCAG...5^EaeI識(shí)別位點(diǎn)5.,.?GGCCR,.33..RtCGG^5’EaeI識(shí)別位點(diǎn)5.../GGCCR,.,3*3..R&CGG^...5JEagI識(shí)別位點(diǎn)5:Mggccg…丁3"qCGGGQ.,.5'EarI識(shí)別位點(diǎn)5:.一CTCTTC(N)「一一3"3:.GAGAAG(N)d一SEciI識(shí)別位點(diǎn)5"GGCGGA<N)|tT..3^3"CCGC0T(N)“_5?EcoNI識(shí)別位點(diǎn)5;?,CCTNN'NNNAGG,?.3一3",GGANN辿NTGC,.3EcoO109I識(shí)別位點(diǎn)5,r■RGi'tNCCY.,.3r3:.¥CCNGJpR...5JEcoP15I識(shí)別位點(diǎn)5;,CAGCAG(N)kt.3*3~..一GTCGTC誦%.一一SEcoRI識(shí)別位點(diǎn)5;MaATTCr3「3:.,CTTAAp..5^EcoRV識(shí)別位點(diǎn)5"一GA『也TC,「G3:..CTAJAG...5^FatI識(shí)別位點(diǎn)..TCN^GA...3rT.,CT一5’Hpy188III識(shí)別位點(diǎn)5:.TC^MNGA...3-3J.AGMNJiT.5JHpy99I識(shí)別位點(diǎn)5F-,-EGWCG*-3"3:.JSCWGO…5’HpyAV識(shí)別位點(diǎn)XCTTClN)i^..3J.GGAAGtN)s...5^HpyCH4III識(shí)別位點(diǎn)..ACN¥GT...3r3一一 一5'HpyCH4IVHpyCH4V識(shí)別位點(diǎn)5".TG’GA…了3一ACQT3KasI識(shí)別位點(diǎn)5;,屆CGGC,,G3:..GCGCGp…羅KpnI識(shí)別位點(diǎn)5",GGTAC".3,3J...嘗而TGG一一亨MboI識(shí)別位點(diǎn)5-,MTG站…CTAG*.3MboII識(shí)別位點(diǎn)5,GAAGA(N)「…3’3\tCTTCT嘰一SMfeI識(shí)別位點(diǎn)礦Maattg,,33J..GT"AQ.一5’MluI識(shí)別位點(diǎn)5r...fCGCGT,r,3^3:..TGGG^A.5MlyI識(shí)別位點(diǎn)5.P.GAGTC(N)^一足3J.CTCAG㈣霍5MmeI識(shí)別位點(diǎn)5:..TCCRAC(NJ^y..33:,,AGG¥TG(N)ia^.J5fMnlI識(shí)別位點(diǎn)5..CCTC恤"3¥.,GGAG(N虹.5MscI識(shí)別位點(diǎn)5:,.TGGitCA,,.3‘.ACCpGT.5J5r...TTTAA...3r3".AATJ.一一亍MslI識(shí)別位點(diǎn)5:.,CAYNNTNNRrG...3*Sp...GTRnN/iNYAC.MspA1I識(shí)別位點(diǎn)5,, KG…丁3...GKG.GMC..5^MspI識(shí)別位點(diǎn)rCTCGGr..y3".GG".3MwoI識(shí)別位點(diǎn)5",GC叫NNNI^NNGC.,?丁/_CGNN^NNNNNCG...S'NaeI識(shí)別位點(diǎn)5;,.GC說(shuō)GC…丁3J.CGG^CG…5’NarI識(shí)別位點(diǎn)S—GGiGGC一3「丁一CCGC^GG..5JNciI識(shí)別位點(diǎn)5"CC%GG-T丁…GGSQC…SNcoI識(shí)別位點(diǎn)5;MeATGG…丁3"GG"¥…5’NdeI識(shí)別位點(diǎn)5..CA^TATG.3^3;..GTA1;AC…5’NgoMIV識(shí)別位點(diǎn)5,.,<?fcCGGC.,.3^3^.CGGCC^G.5JNheI識(shí)別位點(diǎn)5;,.如TAGC…丁3J.CGATC^G…5’NlaIII識(shí)別位點(diǎn)51..CATTGt..3r3"ENlaIV識(shí)別位點(diǎn)5"GG心"一一引3"CG呻GG.,_5‘NmeAIII識(shí)別位點(diǎn)5'..女件膈㈣墓…#'.,CGGCTC而塞%.3NotI識(shí)別位點(diǎn)5:..GC七GCCGC…3’機(jī)一CGCCGG^G..5J5;,.1■。田GA,,33J..AGGpCT.,.5JNsiI識(shí)別位點(diǎn)5;一ATGGfT一3,3:..1;AGGTA…5’NspI識(shí)別位點(diǎn)5:..RCATtfY...3*3"一七$丁山CA__亍PacI識(shí)別位點(diǎn)5t..TTAAT11?AA...3f3J..AATJAATT...5^PaeR7I識(shí)別位點(diǎn)礦,,^TCGAG,,g3"GAGC1;C…5’PciI識(shí)別位點(diǎn)5:"CATGT…33J.TGIACA...5RPflFI識(shí)別位點(diǎn)5;,?GAGN劃NG",..丁3J.CTGiN^NCAG..5JPflMI識(shí)別位點(diǎn)5:.,CCANNHNtNTGG,3*3:.GGTN^NMNNACC.5PhoI識(shí)別位點(diǎn)s;.饑上?!?J..CC^G...5JPleI識(shí)別位點(diǎn)5J...GAGTC(叫「一.一苴3",CTGAG(N),tPmeI識(shí)別位點(diǎn)5:..GTTT^AAC...3*3"CAAAJTTG..5JPmlI識(shí)別位點(diǎn)5,..C^C'GTO..,,r3.GTG^AC..5^PpuMI識(shí)別位點(diǎn)5:..RGTGWCCY,.,3"3J..YCCWG^GR.PshAI識(shí)別位點(diǎn)5"qAONlfNNGTC…3?3J..CTGNhl^NNCAG...5JPsiI識(shí)別位點(diǎn)^...TT^TAA.-rS'3.AA TT..5^PspGI識(shí)別位點(diǎn)5.YCWGG-3^3:.GGWCC^,5R55…GGGCCG,,3*3F..CCCGQp..5^PspXI識(shí)別位點(diǎn)5…V(fTCGAGB…3"3'...BGAGCT^GV..5^PstI識(shí)別位點(diǎn)5「.CTGC^G,?.3「3J.O/CGTC.5^PvuI識(shí)別位點(diǎn)S^.-CQAT^G..3r3"GGJAGC…5’PvuII識(shí)別位點(diǎn)5,3AGtTG…丁"GTQpAC…5’RsaI識(shí)別位點(diǎn)5…GT“C…丁3..-CAJC.-.S^RsrII識(shí)別位點(diǎn)5;,匕由WCGG…了3JGCCWt^lC..5JSacI識(shí)別位點(diǎn)5\..GAGCT^..3r3<..CJCfiAG.5*SacII識(shí)別位點(diǎn)5;,,CCGCPG,3"GGJDGGC...5JSalI識(shí)別位點(diǎn)5\..gVcGAC...3-a^.CAGCTjG..5JSapI識(shí)別位點(diǎn)5P..-GCTCTTC(N>7-,3r3GAGAAG假0‘,5Sau3AI識(shí)別位點(diǎn)5」此TC..?¥*MTAG*5Sau96I識(shí)別位點(diǎn)5-...gTgNCC....3r3:.CCNGp.,5JSbfI識(shí)別位點(diǎn)5r..CCTGC/TGC,,3^3」-GGfGGTCJC.5ScaI識(shí)別位點(diǎn)51,.AGT九C「―丁3..TCRJGA._5‘ScrFI識(shí)別位點(diǎn)5\.CdNGG..,3r3:..GGNMCC.,5J5;,rXfcCWGGT,,.3'3"TGGWGq/一夕SfaNI識(shí)別位點(diǎn)5;,.GCATC(N)『,33..GGTAG(N)Pa..5*SfcI識(shí)別位點(diǎn)5:,.(7TRYAG…3’3J.GAVFtl^C..5'SfiI識(shí)別位點(diǎn)5:.GGCCNNNNYgGCC...3■3J..CCGGN^NNNNCCGG.5-SfoI識(shí)別位點(diǎn)5"GGC饑C-83J.CC^pGG.5JSgrAI識(shí)別位點(diǎn)5"。昨CGGYG—3,.GYGGCCaRC...5^SmaI識(shí)別位點(diǎn)CC<^GGy3"..GGG^CC…亍SmlI識(shí)別位點(diǎn)5:..<TtYRAG...3'3..GARYT^C..5^Sna
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