醫(yī)院實驗室生物安全管理作業(yè)辦法

目 頁目 適用范圍及對 實驗室管 人員管 生物材料保全管 生物材料異動管 7.緊急應變計劃實驗室查核與啟 實施與修 附件一病原體微生物危險性等級分 附件二第二級以上實驗室的生物安全意外事件等級說明、通報規(guī)定 附件三二級生物安全實驗室啟用(新建改建擴建或重新啟用) 實驗室生物安全管理作業(yè)實驗室生物安全管理作業(yè)生物安全意外事件的發(fā)生，特依《實驗室生物安全通用要求》（GB19489-2004）,（GB19781-)新進人員應經(jīng)其實驗室主管或具二年以上操作經(jīng)驗的人員訓練、測試合格，始得操作感染性生物材料。在職人員亦需每年接受實驗室生物安全管理小組每年應擬定年度教育訓練計劃，內(nèi)容至少4事，運送人員應依「感染性物質(zhì)運送作業(yè)準則，;立即通知原實驗理1次教育訓練。甲、第一級危險群（Riskgroup1RG1）的Bacilluslicheniformis,Escherichiacoli-K12,adeno-associatedvirus第一型至第四型，及重組的不含潛在致癌基因產(chǎn)物或毒素的adeno-associatedvirus，且在無輔助病毒存在下進行培養(yǎng)。乙、第二級危險群（Riskgroup2RG2）Acinetobacterbaumannii（AcinetobacterActinomycespyogenes（CorynebacteriumAeromonasAmycolata haemolyticum（以前稱CorynebacteriumArizonahinshawii（所有血清型BacillusBartonellahenselae,B.quintana,B.BordetellaBBorreliarecurrentis,B.Burkholderia（以前稱為Pseudomonasspecies；列于第三級Campylobactercoli,C.fetus,C.Chlamydiapsittaci,C.trachomatis,C.Clostridiumbotulinum,Cl.chauvoei,Cl.haemolyticum,Cl.histolyticum,Cl.novyi,Cl.septicum,Cl.tetaniCorynebacteriumdiphtheriae,C.pseudotuberculosis,C.DermatophilusEdwardsiellaErysipelothrixK1E.coliHaemophilusducreyi,H.HelicobacterKlebsiella（K.oxytoca（RG1）以外的所有菌株LegionellaLLeptospirainterrogans（所有血清型Mycobacterium（除了列于第三級危險群者包括M.aviumcomplexMasiaticum,M.bovisBCGvaccinestrain,M.chelonei,M.fortuitum,M.kansasii,M.leprae,M.malmoense,M.M.paratuberculosis,M.scrofulaceum,M.simiae,M.szulgai,M.ulcerans,M.xenopiMycoplasma（Mmycoides及Magalactiae除外Neisseriagonorrhoeae,N. N.Rhodococcus 包 enteritidis,S.gallinarum-pullorum,S.meleagridis,S.paratyphiA,B,C,S.typhi,S.typhimuriumShigellaS.boydiiSdysenteriaetype1SflexneriS.SphaerophorusStreptococcusS.pneumoniae,SVibriocholerae,V.parahemolyticus,V.YersiniaBlastomycesCladosporiumbantianum,CryptococcusDactylariagalopava（OchroconisFonsecaeaParacoccidioidesPenicilliumSporothrixAncylostomahumanhookwormsAduodenaleA.AscarisAscarislumbricoidesBabesiaB.divergensB.BrugiafilariawormsBmalayiBCryptosporidiumC.Cysticercuscellulosae（hydatidcyst,larvaofT. E.granulosisE.multilocularisEEntamoebaFasciolaFgiganticaFGiardiaGHymenolepisHdiminutaHLeishmaniaL.braziliensis,L.donovani,L.ethiopia,L.major,L.mexicana,L.peruvania,L.tropicaLoaloafilariaNaegleriaNecatorhumanhookwormsNOnchocercafilariawormsO.Plasmodium包括引起猿猴類疾病的P.cynomologi,P.falciparum,P.malariae,P.ovale,P.vivaxSarcocystisSsuiSchistosomaS.haematobium,S.intercalatum,S.japonicum,S.mansoni,S.mekongiStrongyloidesSToxocaraTToxoplasmaTTrypanosoma包括T.bruceibrucei,T.bruceigambiense,T.bruceirhodesiense,T.cruziWuchereriabancroftifilariaAdenoviruses,human-allBunyamweraRiftValleyfevervirusvaccinestrainMP-Coronaviruses（SARS-AssociatedCoronaviruses列于第三級危Denguevirusserotypes1,2,3,andJapaneseencephalitisYellowfevervirusvaccinestrainHepatitisA,B,C,D,andEHerpesviruses（Herpesvirussimiae（MonkeyBvirus）除外EpsteinBarrHerpessimplextypes1andHerpesHumanherpesvirustypes6andInfluenzavirusestypesA,B,andAllhumanpapillomaNewcastlediseaseMeaslesMumpsParainfluenzavirusestypes1,2,3,andRespiratorysyncytialHumanCoxsackievirusestypesAandEchoviruses-allRhinoviruses-all （Monkeypoxvirus及被管制的Alastrim,Smallpox與Whitepox除外）ReovirusesColtivirushumanRotavirus（ColoradotickfeverRabiesvirus-alld.Vesicularstomatitisvirus-VSV-Indiana,SanJuan及Glasgow及丙、第三級危險群（Riskgroup3RG3）BrucellaB.abortusB.canis,B.Burkholderia（Pseudomonas）mallei,B.CoxiellaFrancisellaMycobacteriumbovis（BCG除外）MPasteurellamultocidatypeB-"buffalo"andothervirulentRickettsiaakari,R.australis,R.canada,R.conorii,R.prowazekii,R.rickettsii,R.siberica,R.tsutsugamushi,R.typhi（R.Yersinia(二)Coccidioidesimmitis（sporulatingcultures；contaminatedHistoplasmacapsulatum,H.capsulatumvar.EasternequineencephalomyelitisSemlikiForestSt.LouisencephalitisVenezuelanequineencephalomyelitisvirus（TC-83疫苗株Westernequineencephalomyelitisvirus（LCM（neurotropicHantavirusesHantaanRiftValleyfeverSARS-associatedCoronaviruses(SARS-WestNileYellowfeverPolioviruses-alltypes,wildandMonkeypox encephalopathies（TSEagents（Creutzfeldt-JacobdiseaseandkuruHumanimmunodeficiencyvirus（HIV）types1andHumanTcelllymphotropicvirus（HTLV）types1andSimianimmunodeficiencyVesicularstomatitis丁、第四級危險群（Riskgroup4,RG4）GuanaritoLassaJuninMachupoCrimean-CongohemorrhagicfeverEbolaMarburgFlaviruses（Togaviruses）GroupBArboviruses 包括Absetterov,CentralEuropeanencephalitis,Hanzalova,Hypr,Kumlinge,KyasanurForestdisease,Omskhemorrhagicfever encephalitisvirusesHerpesvirussimiae（HerpesBMonkeyBEquineHemorrhagicfeveragentsandvirusesasyet常RG1murineleukemiavirus的amphotropicxenotropicRG2級的防護等級下進HerpesvirusHerpesvirusMarek’sdiseaseMurineBovinepapillomaPolyoma