GB/T 44365-2024牙膏中6-甲基香豆素、二氫香豆素、7-甲基香豆素、7-甲氧基香豆素、7-乙氧基-4-甲基香豆素的測定高效液相色譜法

ICS

71.100.40

CCS

Y43

中華人民共和國國家標(biāo)準(zhǔn)

GB/T44365—2024

牙膏中6?甲基香豆素、二氫香豆素、

7?甲基香豆素、7?甲氧基香豆素、

7?乙氧基?4?甲基香豆素的測定

高效液相色譜法

Determinationof6?methylcoumarin,dihydrocoumarin,7?methylcoumarin,

7?methoxycoumarin,7?ethoxy?4?methylcoumarinintoothpastes—

Highperformanceliquidchromatography

2024-08-23發(fā)布2025-03-01實(shí)施

國家市場監(jiān)督管理總局發(fā)布

國家標(biāo)準(zhǔn)化管理委員會(huì)

GB/T44365—2024

目次

前言

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Ⅲ

1

范圍

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1

2

規(guī)范性引用文件

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1

3

術(shù)語和定義

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1

4

原理

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1

5

試劑和材料

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1

6

儀器設(shè)備

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2

7

試驗(yàn)步驟

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2

7.1

試樣溶液的制備

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2

7.2

測定

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2

7.3

標(biāo)準(zhǔn)工作曲線

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3

7.4

試樣測定

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3

7.5

空白試驗(yàn)

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3

8

結(jié)果計(jì)算

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3

9

回收率

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4

10

精密度

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4

11

檢測限和定量限

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4

附錄A（資料性）標(biāo)準(zhǔn)物質(zhì)/標(biāo)準(zhǔn)樣品信息

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5

附錄B（資料性）5種香豆素標(biāo)準(zhǔn)溶液的色譜圖和光譜圖

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6

附錄C（資料性）確證試驗(yàn)

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10

C.1液相色譜參考工作條件

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10

C.2質(zhì)譜參考工作條件

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10

C.3定性測試

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11

Ⅰ

GB/T44365—2024

前言

本文件按照GB/T1.1—2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分：標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)

定起草。

請(qǐng)注意本文件的某些內(nèi)容可能涉及專利。本文件的發(fā)布機(jī)構(gòu)不承擔(dān)識(shí)別專利的責(zé)任。

本文件由中國輕工業(yè)聯(lián)合會(huì)提出。

本文件由全國口腔護(hù)理用品標(biāo)準(zhǔn)化技術(shù)委員會(huì)（SAC/TC492）歸口。

本文件起草單位：蘇州世譜檢測技術(shù)有限公司、蘇州質(zhì)量檢測科學(xué)研究院、蘇科世譜生物技術(shù)（蘇州）

有限公司、蘇州市科耐瑞檢測技術(shù)有限公司、廣州舒客實(shí)業(yè)有限公司、廣州質(zhì)量監(jiān)督檢測研究院、黑龍

江省輕工科學(xué)研究院、江西誠志日化有限公司、無限極（中國）有限公司、柳州兩面針股份有限公司、

重慶登康口腔護(hù)理用品股份有限公司、河北省食品檢驗(yàn)研究院、江蘇省產(chǎn)品質(zhì)量監(jiān)督檢驗(yàn)研究院、江蘇

省食品藥品監(jiān)督檢驗(yàn)研究院、完美（廣東）日用品有限公司、清華大學(xué)、海南大學(xué)、深圳大學(xué)、

蘇州大學(xué)、常州大學(xué)、常州市食品藥品纖維質(zhì)量監(jiān)督檢驗(yàn)中心、蘇州市藥品檢驗(yàn)檢測研究中心、山東華

素健康護(hù)理品有限公司。

本文件主要起草人：葉嘉榮、盧劍、張巖、方方、李建祥、鄭衛(wèi)、葉竹洪、趙姍姍、陳敏珊、

許海燕、高艷、楊衛(wèi)豪、陳欣、肖俊芳、秦天依、劉斌、結(jié)莉、吳玉鑾、代丹、朱丹丹、王怡、

李珮?biāo)?、張迎陽、侯向昶、宋淑文、聶炎炎、盧應(yīng)紅、姜雷、高龍、顧盼、劉群林、高紅影、朱釔蓉、

唐文金。

Ⅲ

GB/T44365—2024

牙膏中6?甲基香豆素、二氫香豆素、

7?甲基香豆素、7?甲氧基香豆素、

7?乙氧基?4?甲基香豆素的測定

高效液相色譜法

警示─使用本文件的人員應(yīng)有正規(guī)實(shí)驗(yàn)室工作的實(shí)踐經(jīng)驗(yàn)。本文件并未指出所有可能的安全問題。

使用者有責(zé)任采取適當(dāng)?shù)陌踩徒】荡胧?，并保證符合國家有關(guān)規(guī)定的條件。

1范圍

本文件描述了牙膏中6?甲基香豆素、二氫香豆素、7?甲基香豆素、7?甲氧基香豆素、7?乙氧基?4?甲

基香豆素高效液相色譜測定方法的原理、試劑和材料、儀器設(shè)備、試驗(yàn)步驟、結(jié)果計(jì)算、回收率和精密

度、允許差等內(nèi)容。

本文件適用于牙膏中6?甲基香豆素、二氫香豆素、7?甲基香豆素、7?甲氧基香豆素、7?乙氧基?4?甲

基香豆素的測定。

2規(guī)范性引用文件

下列文件中的內(nèi)容通過文中的規(guī)范性引用而構(gòu)成本文件必不可少的條款。其中，注日期的引用文

件，僅該日期對(duì)應(yīng)的版本適用于本文件；不注日期的引用文件，其最新版本（包括所有的修改單）適用

于本文件。

GB/T6682分析實(shí)驗(yàn)室用水規(guī)格和試驗(yàn)方法

3術(shù)語和定義

本文件沒有需要界定的術(shù)語和定義。

4原理

牙膏中6?甲基香豆素、二氫香豆素、7?甲基香豆素、7?甲氧基香豆素、7?乙氧基?4?甲基香豆素經(jīng)甲

醇提取后，采用高效液相色譜?二極管陣列檢測器檢測，以保留時(shí)間和光譜圖定性，外標(biāo)法定量。

5試劑和材料

5.1除另有說明，所用試劑均為色譜純，水為符合GB/T6682要求的一級(jí)水。

5.2