醫(yī)學(xué)分子醫(yī)學(xué)概論

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2、AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAcDNA synthesisInfect cellscDNA library建 庫(kù)篩選核糖體展示核糖體展示固相化靶分子固相化靶分子體外展示系統(tǒng)GeneBindingDomainActivationDomainReporter GeneBaitProteinBindingDomainPrey ProteinActivationDomainThe Two-Hybrid System融合基因報(bào)道株Reporter GeneBaitProteinBindingDomainPrey ProteinActivationDomainDi

3、cersiRNAInitiation StepInitiation StepRISCInitiation StepATPATPADP + ppiADP + ppiDICERKINASERdRPEffector Step siRNA binding siRNA unwinding RISC activationVectors expressing siRNAsU6Sense sequenceAnti-sense sequenceHair-pin loopSense sequenceAnti-sense sequenceMedium-density DNA microarrayfluorescence imageslDNA array with a distance of 400 um of center-to-center lSYBR stains ssDNA probe (Up)lCy5 (red) and Fluor X (Green) stain immobilized DNA probes (Right)Array Area: 0.6 cm x 0.3 cm 克隆羊克隆羊“ Vectors expressing siRNAsU6Sense sequenceAnti-