細(xì)環(huán)病毒從實(shí)驗(yàn)室到臨床(1)

1、整理課件1細(xì)環(huán)病毒：從實(shí)驗(yàn)室到臨床細(xì)環(huán)病毒：從實(shí)驗(yàn)室到臨床Torquetenovirus: the human virome from bench to bedsideTorquetenovirus: the human virome from bench to bedside D. Focosi et al. Clinical Microbiology and Infection 22 (2016) 589-593整理課件2TTV: Transfusion transmitted virus or TorquetenovirusFrom torques and tenuis, Latin f

2、or necklace and thin, respectively輸血后肝炎（肝中病毒載量是血中的輸血后肝炎（肝中病毒載量是血中的10-100倍，并且與血清倍，并且與血清ALT升高相關(guān)）升高相關(guān)）【現(xiàn)已否現(xiàn)已否定該結(jié)論定該結(jié)論】Human viromeat least 29 major species細(xì)環(huán)病毒：首個(gè)被發(fā)現(xiàn)的單鏈環(huán)狀細(xì)環(huán)病毒：首個(gè)被發(fā)現(xiàn)的單鏈環(huán)狀DNA病毒病毒整理課件3TTV prevalence 95% in most cases independent of age, socioeconomic standing and health conditions來(lái)源：來(lái)源：胎盤(pán)、

3、呼吸道、輸血胎盤(pán)、呼吸道、輸血分布：分布：T淋巴細(xì)胞淋巴細(xì)胞現(xiàn)有臨床應(yīng)用：現(xiàn)有臨床應(yīng)用：TTV as a Marker of Anthropic PollutionTTV as a Marker of Immune StatusOrphan virus孤兒病毒（非病原性病毒）孤兒病毒（非病原性病毒）整理課件4檢測(cè)水源是否被污染：檢測(cè)水源是否被污染：現(xiàn)有方法為檢測(cè)細(xì)菌，但是很容易出現(xiàn)假陽(yáng)性現(xiàn)有方法為檢測(cè)細(xì)菌，但是很容易出現(xiàn)假陽(yáng)性某些情況下即使細(xì)菌檢測(cè)為陰性也不能排除被污染某些情況下即使細(xì)菌檢測(cè)為陰性也不能排除被污染檢測(cè)病毒更準(zhǔn)確檢測(cè)病毒更準(zhǔn)確TTV分布廣泛：醫(yī)院空氣、物件表面均可存在分布廣泛：醫(yī)

4、院空氣、物件表面均可存在TTV as a Marker of Anthropic PollutionTTV as a Marker of Immune Status免疫功能完整性：免疫功能完整性： the ability to mount a protective immune response against an antigenic stimulation.現(xiàn)有檢測(cè)：抗原特異性，抗原非特異性；檢測(cè)繁瑣，檢測(cè)方法缺乏標(biāo)準(zhǔn)化現(xiàn)有檢測(cè)：抗原特異性，抗原非特異性；檢測(cè)繁瑣，檢測(cè)方法缺乏標(biāo)準(zhǔn)化TTV與與CD8+57+T淋巴細(xì)胞比例成負(fù)相關(guān)淋巴細(xì)胞比例成負(fù)相關(guān)對(duì)抗病毒藥不敏感對(duì)抗病毒藥不敏感整理課件5

5、TTV as a Marker of Immune Statusexcessive (too many opportunistic infections and cancers) inadequate (too many episodes of cellular acute rejection) Maintenance immunosuppression整理課件6Why Is TTV Not Yet Part of a Diagnostic Tool Kit?現(xiàn)有研究不足現(xiàn)有研究不足無(wú)法培養(yǎng)無(wú)法培養(yǎng)無(wú)抗體檢測(cè)試劑無(wú)抗體檢測(cè)試劑無(wú)動(dòng)物模型無(wú)動(dòng)物模型PCR方法缺乏標(biāo)準(zhǔn)化（引物設(shè)計(jì)）方法缺乏標(biāo)準(zhǔn)化（引物設(shè)計(jì)）特異性引物特異性引物非特異性引物非特異性引物整理課件7Take home messagesTTV is a type of Orphan virus, prevalence95% in most casesTTV replicates mainly in T lymphocytesInsensitive to current antiviral drugs TTV as a Marker of Immune StatusPredictin