NEB 同尾酶表

1、連接互相匹配的粘性末端，產(chǎn)生新的酶切位點(diǎn)通常連接互相匹配的粘性末端可以產(chǎn)生新的酶切位點(diǎn)。以下組合是通過計(jì)算機(jī)程序計(jì)算出來的，盡管我們希望能盡量保證其準(zhǔn)確性，但有些未經(jīng)實(shí)驗(yàn)證實(shí)，因此不能保證100%的可行性。如果有同裂酶存在，則只列出其中的一個(gè)酶。本表中列出的內(nèi)切酶均為已商品化的內(nèi)切酶。識(shí)別序列不夠嚴(yán)謹(jǐn)?shù)膬?nèi)切酶（例如，識(shí)別多個(gè)序列的酶）只在括號(hào)內(nèi)標(biāo)出相關(guān)的序列，但注意這些酶不只切割該序列。"-"表示連接產(chǎn)物不能被再切割。酶連接到可進(jìn)行切割的酶Acc65 I (G/GTACC)Ban I (G/GTACC)Acc65 I, Ban I, Kpn I, Nla IV, Rsa I

2、 BsiW I, BsrG IRsa IAcc I(GT/CGAC)Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I-(GT/CGAC)Cla I, BstB I, Taq ITaq IAci I(C/CGC) Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-BsaH I (GR/CGCC), HinP1 I, Nar IAci IHpa IIHpa IIAcl I (AA/CGTT)Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hp

3、a II, Nar I, Taq I-Afl III(A/CGCGT)Asc I, BssH IIBstU I, Hha I(A/CATGT)BspH I, Nco I, Pci INla III(A/CGCGT)Mlu IAfl III, BstU I, Mlu IAge I (A/CCGGT)Ava I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBsaW I, BspE IBsaW I, Hpa IIBsrF I (A/CCGGT), SgrA I (CA/CCGGTG)Age I, BsaW I, BsrF I, Hpa IINgoM IVBsrF I,

4、Hpa IIApa I (GGGCC/C)Ban II (GGGCC/C), Bsp1286 I (GGGCC/C)Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV, Sau96 IApaL I (G/TGCAC)Sfc I (C/TGCAG)Bsg IApo I(A/AATTY)EcoR IApo I, Tsp509 I(G/AATTY)EcoR IApo I, EcoR I, Tsp509 I(R/AATTY)Mfe I, Tsp509 ITsp509 IAsc I (GG/CGCGCC)Afl III (A/CGCGT), Mlu I

5、BstU I, Hha IBssH IIBssH II, BstU I, Cac8 I, Hha IAse I (AT/TAAT)Bfa I, Csp6 I, Nde I-Mse IMse IAsiS I(GCGAT/CGC) BsiE I (CGAT/CG)Dpn II, Pvu IPac IMse IPvu IDpn II, Pvu IAva I(C/CCGGG)Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I (CR/CCGGYG)Hpa II, Nci I, ScrF I(C/TCGAG)Xho IAva I, Taq I

6、, Xho I(C/TCGAG)Sal ITaq I(C/CCGGG)Xma IAva I, BsaJ I, Hpa II, Nci I, ScrF I, Sma IAva II (G/GWCC)PpuM I (RG/GACCY)Ava II, Nla IV, Sau96 IRsr IIAva II, Sau96 IPpuM I (RG/GTCCY)Ava II, BsmF I, Nla IV, Sau96 IAvr II (C/CTAGG)Nhe I, Spe I, Xba IBfa ISty I (C/CTAGG)Avr II, Bfa I, BsaJ I, Sty IBamH I (G/

7、GATCC)Bcl I, Dpn IIAlw I, Dpn IIBgl II, BstY I (R/GATCY)Alw I, BstY I, Dpn IIBstY I (G/GATCC)Alw I, BamH I, BstY I, Dpn II, Nla IVBan I(G/GTACC)Acc65 IAcc65 I, Ban I, Kpn I, Nla IV, Rsa I(G/GCGCC)Kas IBan I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV(G/GTACC)BsiW I, BsrG IRsa IBan II(GGGCC/C)Apa I,

8、 Bsp1286 I (GGGCC/C)Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GAGCT/C)Bsp1286 I (GAGCT/C), Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac IBcl I(T/GATCA) BamH I, BstY I (R/GATCY)Alw I, Dpn IIBgl II, Mbo IIDpn IIBfa I (C/TAG)Ase I, Csp6 I, Mse I, Nde I-Bgl II (A/GATCT)BamH I, BstY I (R/GATCY)A

9、lw I, BstY I, Dpn IIBcl I, Dpn IIDpn IIBsaH I(GR/CGYC)Acc I (GT/CGAC), Cla I, BstB I, Taq I-(GA/CGYC)Aci I, HinP1 IHga I(GG/CGYC)Aci I, HinP1 IHha I(GG/CGYC)Hpa IIAci I(GA/CGYC)Nar IBsaH I, Hga I(GG/CGYC)Nar IBan I, BsaH I, Hae II, Hha I, Nar I, Nla IVBsaW I (W/CCGGW)Age I, BsrF I (R/CCGGY), SgrA I

10、(CR/CCGGYG)Age I, BsaW I, BsrF I, Hpa IIAva I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBspE IBsaW I, BspE I, Hpa IIBsrF I (R/CCGGY), NgoM IVBsrF I, Hpa IINgoM IVHpa IIBsiE I(CGAT/CG)Pac IMse I(CGAT/CG)Pvu IBsiE I, Dpn II, Pvu I(CGGC/CG)Sac IIAci IBsiHKA I(GTGCA/C) Bsp1286 I (GTGCA/C)BsiHKA I, Bsp1286 IB

11、sp1286 I (GAGCA/C)BsiHKA I, Bsp1286 IBsp1286 I (GAGCT/C), Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac INsi I-Pst I, Sbf IBsg IBsiW I (C/GTACG)Acc65 I, Ban I (G/GTACC), BsrG IRsa IBsp1286 I(GGGCC/C)Apa I, Ban II (GGGCC/C)Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GTGCA/C)BsiHKA IApaL I, BsiH

12、KA I, Bsp1286 I(GGGCC/C)Ban II (GGGCC/C)Ban II, Bsp1286 I(GAGCT/C)Ban II (GAGCT/C), BsiHKA I, Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac I(GWGCW/C)BsiHKA IBsiHKA I, Bsp1286 I(GTGCA/C)Nsi I-(GTGCA/C)Pst I, Sbf IBsg IBspE I (T/CCGGA)Age I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)BsaW I, Hpa IIAv

13、a I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBsaW IBsaW I, BspE I, Hpa IIBsrF I (R/CCGGY), NgoM IVHpa IIBspH I (T/CATGA)Afl III (A/CATGT), Nco I, Sty I (C/CATGG)Nla IIIBsrF I(A/CCGGY)Age I, BsaW IAge I, BsaW I, BsrF I, Hpa II(G/CCGGY)Age I, BsaW I, NgoM IVBsrF I, Hpa II(R/CCGGY)Ava I (C/CCGGG), Xma IHpa

14、 II, Nci I, ScrF I(A/CCGGY)BsaW I, BspE IBsaW I, Hpa II(R/CCGGY)BsaW I, BspE IHpa II(G/CCGGY)NgoM IVBsrF I, Cac8 I, Hpa II, Nae I(CR/CCGGYG)SgrA IBsrF I, Hpa IIBsrG I (T/GTACA)Acc65 I, Ban I (G/GTACC), BsiW IRsa IBssH II (G/CGCGC)Afl III (A/CGCGT), Mlu IBstU I, Hha IAsc IBssH II, BstU I, Cac8 I, Hha

15、 IBstB I (TT/CGAA)Acc I (GT/CGAC), Cla I, Taq ITaq IAci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I-BstY I(A/GATCY)BamH I, Bgl IIAlw I, BstY I, Dpn II(G/GATCY)BamH IAlw I, BamH I, BstY I, Dpn II, Nla IV(R/GATCY)Bcl I, Dpn IIDpn II(G/GATCY)Bcl I, Dpn IIAlw I, Dpn II(A/GATCY)Bgl IIBgl II, BstY

16、I, Dpn IICla I (AT/CGAT)Acc I (GT/CGAC), BstB I, Taq ITaq IAci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I-Dpn II/Mbo I/ Sau3A I (/GATC)BamH I, BstY I (R/GATCC)Alw I, Dpn IIBcl I, Bgl II, BstY I (R/GATCY)Dpn IIEae I(Y/GGCCR)PspOM IHae III, Sau96 I(C/GGCCR)Eag IBsiE I, Eae I, Eag I, Hae III(T/

17、GGCCR)Eag IEae I, Hae III(C/GGCCR)Not IAci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III(T/GGCCR)Not IAci I, Eae I, Fnu4H I, Hae IIIEag I (C/GGCCG)PspOM IHae III, Sau96 IEae I (Y/GGCCR)Eae I, Hae IIIEae I (C/GGCCG)BsiE I, Eae I, Eag I, Hae IIINot IAci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae IIIEcoR I (G/A

18、ATTC)Apo I (G/AATTC)Apo I, EcoR I, Tsp509 IApo I (R/AATTY)Apo I, Tsp509 IMfe I, Tsp509 ITsp509 IHinP1 I (G/CGC)Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-Aci I, BsaH I (GR/CGCC), Nar IHha IBsaH I (GR/CGTC)Hga IHpa IIAci IHpa II/Msp I (C/CGG)Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-Aci I, BsaH

19、I (GR/CGCC), HinP1 I, Nar IAci IKas I (G/GCGCC)Ban I (G/GCGCC)Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IVMfe I (C/AATTG)Apo I (R/ATTTY), EcoR I, Tsp509 ITsp509 IMlu I (A/CGCGT)Afl III (A/CGCGT)Afl III, BstU I, Mlu IAsc I, BssH IIBstU I, Hha IMse I (T/TAA)Ase IMse IBfa I, Csp6 I, Nde I-Nar I (

20、GG/CGCC)Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-Aci I, HinP1 IHha IBsaH I (GR/CGCC)Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IVBsaH I (GR/CGTC)BsaH I, Hga IHpa IIAci INco I (C/CATGG)Afl III (A/CATGT), BspH INla IIISty I (C/CATGG)BsaJ I, Nco I, Nla III, Sty IPci INla IIINde I (CA/TATG)Ase I, Bfa

21、I, Csp6 I, Mse I-NgoM IV (G/CCGGC)Age I, BsaW I, BsrF I (R/CCGGY), SgrA IBsrF I, Hpa IIAva I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBsaW I, BspE IHpa IIBsrF I (R/CCGGC), SgrA IBsrF I, Cac8 I, Hpa II, Nae INhe I (G/CTAGC)Avr II, Spe I, Sty I (C/CTAGG), Xba IBfa INla III (CATG/)Sph I, Nsp INla IIINot I

22、(GC/GGCCGC)PspOM IAci I, Eae I, Fnu4H I, Hae IIIEag IAci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae IIIEae I (Y/GGCCR)Aci I, BsiE I, Eae I, Fnu4H I, Hae IIINsi I (ATGCA/T)BsiHKA I (GTGCA/C), Bsp1286 I (GTGCA/C), Pst I, Sbf I-Nsp I (RCATG/Y)Nla III, Sph INla IIIPac I (TTAAT/TAA)BsiE I (CGAT/CG), Pvu IMse

23、IPci I (A/CATGT)Afl II (A/CATGT), BspH I, Nco I, Sty I (C/CTAGG)Nla IIIPpuM I(RG/GWCCY)Ava II, Rsr IIAva II, Sau96 I(GG/GTCCY)Ava II, Rsr IIAva II, BsmF I, Nla IV, Sau96 I(GG/GACCY)Ava II, Rsr IIAva II, Nla IV, Sau96 IPspOM I (G/GGCCC)Eae I (Y/GGCCR), Eag IHae III, Sau96 INot IAci I, Fnu4H I, Hae II

24、I, Sau96 IPst I (CTGCA/G)BsiHKA I, Bsp1286 I (GTGCA/C)Bsg INsi I-Sbf IPst IPvu I (CGAT/CG)AsiS IDpn I, Pvu IPac IMse IBsiE I (CGAT/CG)BsiE I, Dpn II, Pvu IRsr II (CG/GWCCG)Ava II, PpuM I (RG/GACCY)Ava II, Sau96 IPpuM I (RG/GACCY)Ava II, Nla IV, Sau96 IPpuM I (RG/GTCCY)Ava II, BsmF I, Nla IV, Sau96 ISac I (GAGCT/C)Ban II (GAGCT/C), BsiHKA I, Bsp1286 I (GAGCT/C)Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac ISac II (CCGC/GG)BsiE I (CGGC/CG)Aci