《納米生物技術(shù)》教學(xué)大綱（英文版）

1、NanobiotechnologyCourse SyllabusCourse Code：09040009Course Category：Major ElectiveMajors：Chemistry (Intensive Training Class)Semester：FallTotal Hours：36 Hours Credit：2 學(xué)分Lecture Hours：36 Hours Lab Hours：0 Hours Practice Hours：0 HoursTextbooks：Nanobiotechnology: Concepts, Applications and Perspective

2、s, WILEY-VCH Edited by C. M. Niemeyer, C. A. MirkinReferences：1. X. Michalet, F. F. Pinaud, L. A. Bentolila, J. M. Tsay, S. Doose, J. J. Li, G. Sundaresan, A. M. Wu, S. S. Gambhir, S. Weiss,Science 2005, 307, 538.2. I. L. Medintz, H. T. Uyeda, E. R. Goldman, H. Mattoussi, Nat. Mater. 2005, 4, 435.3.

3、 Mirkin, C. A.; Letsinger, R. L.; Mucic, R. C.; Storhoff, J. J. Nature 1996, 382, 607609.4. N. L. Rosi, D. A. Giljohann, C. S. Thaxton, A. K. Lytton-Jean, M. S. Han, C. A. Mirkin, Science 2006, 312, 1027.5. A. V. Pinheiro, D. Han, W. M. Shih, H. Yan Nat. Nanotechnol. 2011, 6, 763.Teaching Aim： This

4、course will provide a general introduction to the newly emerging field of nanobiotechnology. This is a highly interdisciplinary field covering nanotechnology, chemistry, biology, and medicine. The rapid development of this field has lead to a variety of nano architectures that could be applied to bi

5、osensing, molecular imaging, and therapy, which is going to change our lives significantly. This is an advanced course for fourth-year students who have a solid background of chemistry and biochemistry, and those plan to purse a higher degree in the related field. Chapter I Introduction課時：1周，共2課時Con

6、tents第一節(jié) Introduction一、Definition of nanotechnology and nanobiotechnology 二、Classification and preparation of nanomaterials三、Characterization methods四、Journals and search engine思考題 Problems：1、What is nanobiotechnology? 2、What is the difference between TEM, AFM and SEM？3、Please name five top journals

7、 publishing research in the field of nanobiotechnologyChapter II Quantum Dots課時：6周，共12課時Contents第一節(jié) Synthesis and properties一、Discovery of colloidal quantum dots 二、Quantum confinement effect三、Optical properties四、Synthetic methods 第二節(jié) Types and compositions一、Binary and ternary quantum dots二、Type I an

8、d type II quantum dots三、Doped quantum dots四、Strain tuning第三節(jié) Biofunctionalization一、Molecules for solubilization and functionalization二、Aqueous and one-step synthesis 第四節(jié) QDs for biosensing一、Fluorescence resonance energy transfer二、Nucleic acids detection三、Protease detection四、Bioluminescence resonance

9、 energy transfer第五節(jié) QDs for bioimaging一、Molecular recognition ligands 二、Intracellular delivery三、Design consideration for in vivo imaging四、Tumor imaging strategies五、Lymph node imaging六、Drug delivery and cancer therapy思考題 Problems：1、What is quantum confinement effect and how does it determine the phot

10、oluminescence properties of QDs？ 2、Please design a FRET-based QD probe for sensitive detection of specific DNA molecules.3、What is the difference between passive and active tumor targeting？Chapter III Gold nanoparticles課時：3周，共6課時Contents第一節(jié) Synthesis and properties一、Discovery of gold nanoparticles二、

11、Synthesis of gold nanoparticles, nanorods, and nanocages三、Optical properties of gold nanostructures第二節(jié) GNPs for biosensing一、Surface plasma resonance 二、Protease detection三、Nucleic acids detection四、Small molecule detection五、Surface enhanced Raman scattering第三節(jié) GNPs for drug delivery一、Surface functiona

12、lization二、Drug encapsulation and release三、Photothermal therapy思考題 Problems：1、What is surface plasma resonance of gold nanoparticles？ 2、Please design a DNA detection method based on gold nanoparticle aggregation3、Please design a FRET-based gold nanoparticle probe for protease detectionChapter IV DNA

13、nanotechnology課時：3周，共6課時第一節(jié) DNA self-assembly一、DNA chemical structure二、Secondary structure and assembly building blocks三、DNA-templated nanoparticle assembly四、DNA origami五、Dynamic assembly第二節(jié) Applications一、Biosensing二、Drug delivery思考題 Problems：1、What are the advantages of DNA molecules for nanoscale assembly？