WST-8 _Biochemical Assay Reagent - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEWST-8Cat. No.: HY-D0831CAS No.: 193149-74-5分式: CHNNaOS分量: 601.48作靶點(diǎn): Others作通路: Others儲(chǔ)存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn) H2O : 30 mg/mL (49.88 mM)* means soluble,

2、 but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 1.6626 mL 8.3128 mL 16.6257 mL5 mM 0.3325 mL 1.6626 mL 3.3251 mL10 mM 0.1663 mL 0.8313 mL 1.6626 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 WST-8種溶性的四唑染料，相對(duì)于常規(guī)MTS檢測(cè)，WTS-8測(cè)定法的靈敏度增強(qiáng)。體外研究The generally u

3、sed MTS-based assay is compared with a bioassay employing a water-soluble tetrazoliumdye, WST-8, using NFS-60 cells at a concentration of 7105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, theoptimized WST-8 d

4、ye-based assay is used to test the potency of various commercially available PEGylatedG-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEconventional MTS-based assay for determining the potency of PEGylat

5、ed G-CSF using the NFS-60 cell line1.REFERENCES1. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. PharmBiol. 2015 Jun;53(6):849-54.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 400-820-3792; 021-589