C29-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEC29Cat. No.: HY-100461CAS No.: 363600-92-4分式: CHNO分量: 285.29作靶點(diǎn): Toll-like Receptor (TLR)作通路: Immunology/Inflammation儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 30 mg/mL (105.16 mM)* me

2、ans soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 3.5052 mL 17.5260 mL 35.0521 mL5 mM 0.7010 mL 3.5052 mL 7.0104 mL10 mM 0.3505 mL 1.7526 mL 3.5052 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前請先配制澄的?chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建

3、議您現(xiàn)現(xiàn)配，當(dāng)天使；澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (8.76 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (8.76 mM); Clear solution3. 請依序添加每種溶劑： 10% DMSO 90% corn oil1/2 Master

4、of Small Molecules 您邊的抑制劑師www.MedChemESolubility: 2.5 mg/mL (8.76 mM); Precipitated solutionBIOLOGICAL ACTIVITY物活性 C29Toll樣受體2 (Toll-like receptor 2 (TLR2) 抑制劑。IC50 & Target TLR2 1體外研究 C29 blunts hTLR2/1 and hTLR2/6 signaling in HEK-TLR2 Stable transfectants and THP-1 Cells. C29 blocksP3C- and P2C-i

5、nduced IL-8 mRNA dose-dependently in HEK-TLR2 stable transfectants. C29 also inhibitsP3C- and P2C-induced IL-1 gene expression significantly at both 1 h and 4 h following stimulation, as wellas both P3C- and P2C-induced NF-Bluciferase activity in transiently transfected HEK293T cells expressinghTLR2

6、 and an NF-Bsensitive luciferase reporter construct. C29 preferentially inhibits TLR2/1 signaling inprimary murine macrophages. C29 blocks TLR2 bacterial agonist-induced proinflammatory gene expressionin HEK-TLR2 Cells and murine macrophages. C29 inhibits ligand-induced interaction of TLR2 with MyD8

7、8and blocks MAPK and NF-B activation 1. J Invest Dermatol. 2019 Jun 10. pii: S0022-202X(19)31634-3. Carbohyd Polym. 2019 Sep. J Biol Chem. 2019 Jul 19. pii: jbc.RA119.009584. Neurosci Lett. 2018 Nov 1;686:33-40.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Vogel S, et al. Sma

8、ll molecule inhibitors of tlr2 signaling. WO2016164414A12. Mistry P, et al. Inhibition of TLR2 signaling by small molecule inhibitors targeting a pocket within the TLR2 TIR domain. Proc Natl AcadSci U S A. 2015 Apr 28;112(17):5455-60.McePdfHeightCaution: Product has not been fully validated for medical applications.Fo