Laminaran-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemELaminaranCat. No.: HY-119109CAS No.: 9008-22-4作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mL (Need ultrasonic)請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)

2、實(shí)驗(yàn) 請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前?qǐng)先配制澄的儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (Infinity mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (Infinity mM); Clear

3、 solutionBIOLOGICAL ACTIVITY物活性 Laminaran種中性溶性 -1-3-葡聚糖，從褐藻雙歧桿中分離，Dectin-1 的典型配體，具有強(qiáng)的免疫調(diào)節(jié)，輻射防護(hù)作 ，以及抗癌活性。 Laminaran 由具有 (16) 分的 (13)-葡聚糖組成，可以被葡糖酶諸如 (EC ) 之類的酶催化，該酶會(huì)破壞 (13) 鍵。 Laminaran種有前途的免疫刺激分，可于癌癥免疫治療。體外研究Laminaran (100-800 g/mL; 24 hours) is not cytotoxic against normal epidermal cells JB6 Cl41 a

4、nd humanmelanoma cells SK-MEL-28, the percentage of inhibition of living cells number is less than 15 % atconcentrations range up to 800 g/mL after 24 h of treatment 1.Laminaran (200 g/mL; 24-72 hours)doesnot cause any growth inhibition of SK-MEL-28 cells after 24 and 48 h of treatment, but decrease

5、s cellsproliferation by 36 % after 72 h of treatment 1.Laminaran (25-50 g/mL; 24 hours) at low concentration does1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemEnot influence the phosphorylation of c-Raf (Ser259), ERK1/2 (Tyr202/Tyr204), and MEK1/2 (Ser 221) kinasesas well as total expression level

6、 of investigated proteins. But decreases p-MEK, p-ERK1/2 at 50 g/mL 1.Cell Viability Assay 1Cell Line: JB6 Cl41 and SK-MEL-28 cellsConcentration: 100, 200, 400, and 800 g/mLIncubation Time: 24 hoursResult: Showed no cytotoxic against normal epidermal cells JB6 Cl41 and human melanomacells SK-MEL-28.

7、Cell Proliferation Assay 1Cell Line: SK-MEL-28 cellsConcentration: 200 g/mLIncubation Time: 24 hoursResult: Decreased cell proliferation at 72 hours.Western Blot Analysis 1Cell Line: SK-MEL-28 cellsConcentration: 25 g/mL; 50 g/mLIncubation Time: 24 hoursResult: Inhibited phosphorylation of c-Raf, ME

8、K1/2, and ERK1/2 kinases.體內(nèi)研究 Laminaran (intravenous injection; 12.5, 25, and 50 mg/kg; 21 days) and OVA (50 g) combination significanlydecreases the tumor masses when it compares with the PBS-, OVA-, and laminarin-treated mice 3.Animal Model: C57BL/6 mice injected s.c. with B16-OVA cells 3Dosage: 1

9、2.5, 25, and 50 mg/kg; 21 daysAdministration: Intravenous injectionResult: Prevented B16-OVA tumor growth by inducing Ag-specific immune responses.REFERENCES1. Malyarenko OS, et al. Laminaran from brown alga Dictyota dichotoma and its sulfated derivative as radioprotectors and radiosensitizers2/3 Ma

10、ster of Small Molecules 您邊的抑制劑師www.MedChemEin melanoma therapy.Carbohydr Polym. 2019 Feb 15;206:539-547.2. Laminaran3. Song K, et al. Laminarin promotes anti-cancer immunity by the maturation of dendritic cells.Oncotarget. 2017 Jun 13;8(24):38554-38567.McePdfHeightCaution: Product has not been fully valida