BC-1215-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEBC-1215Cat.No.:HY-117937CASNo.:1507370-20-8分?式:C??H??N?分?量:394.51作?靶點:LigandsforE3Ligase作?通路:PROTAC儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:12.5mg/mL(31.68mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.5348mL12.6740mL25.3479mL5mM0.5070mL2.5348mL5.0696mL10mM0.2535mL1.2674mL2.5348mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥1.25mg/mL(3.17mM);Clearsolution1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥1.25mg/mL(3.17mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥1.25mg/mL(3.17mM);ClearsolutionBIOLOGICALACTIVITY?物活性BC-1215?種F-box蛋?3(Fbxo3)抑制劑。BC-1215通過拮抗Fbxo3對TRAF細胞因?信號傳導(dǎo)起作?，并在體外表現(xiàn)出低IC50。BC-1215可?于炎癥研究。體外研究BC-1215(0,0.4,2,10,50μg/mL;16h,18hand24h)inhibitstheFbxo3-TRAFactivationpathwaybydestabilizingTRAF1-6[1].WesternBlotAnalysis[1]CellLine:Murinelungepithellal(MLE)cells,PBMCcellsandU937cellsConcentration:0,0.4,2,10,50μg/mLIncubationTime:6h,18hand24hResult:DecreasedTRAFproteinandinhibitedabroadspectrumofTh1panelcytokines.體內(nèi)研究BC-1215(i.p.;100μg)reducesbacterial-inducedinflammationa[1].AnimalModel:Cecalligationandpuncture(CLP)-inducedsepsismodel[1]Dosage:100μgAdministration:i.p.Result:SignificantlyattenuatedCLP-inducedsecretionofallthreecirculatingpro-inflammatorycytokinesanddecreasedbacterialcountsintheCLPmodel.戶使?本產(chǎn)品發(fā)表的科研?獻?IntJMolSci.2022Nov7;23(21):13648.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].ChenBB,etal.AcombinatorialFboxproteindirectedpathwaycontrolsTRAFadaptorstabilitytoregulateinflammation.NatImmunol.2013May;14(5):470-9.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEMcePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresear