D-Galactose-D-plus-Galactose-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemED-GalactoseCat.No.:HY-N0210CASNo.:59-23-4Synonyms:D-(+)-Galactose分?式:C?H??O?分?量:180.16作?靶點:EndogenousMetabolite作?通路:MetabolicEnzyme/Protease儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗H2O:62.5mg/mL(346.91mM;ultrasonicandwarmingandheatto60°C)DMSO:50mg/mL(277.53mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM5.5506mL27.7531mL55.5062mL5mM1.1101mL5.5506mL11.1012mL10mM0.5551mL2.7753mL5.5506mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(13.88mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(13.88mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(13.88mM);Clearsolution4.請依序添加每種溶劑：PBSSolubility:100mg/mL(555.06mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY?物活性D-Galactose天然的?糖，葡萄糖的C-4差向異構(gòu)體。IC50&TargetHumanEndogenousMetabolite體外研究Galactoseisimportantforthesurvivalandvirulenceofbacteria.InEscherichiacoligalactoseisutilizedbytheLeloirpathway.Twoanomersofd-galactoseareusedfordifferentpurposes,α-d-galactoseasacarbonsourceandβ-d-galactoseforinductionofUDP-galactosesynthesisforbiosyntheticglycosylation[1].體內(nèi)研究ChronicD-galactoseexposureinducesneurodegenerationbyenhancingcaspase-mediatedapoptosisandinhibitingneurogenesisandneuronmigrationinmice,aswellasincreasingoxidativedamage.Inaddition,D-galactose-inducedtoxicityinmiceisausefulmodelforstudyingthemechanismsofneurodegenerationandneuroprotectivedrugsandagents[2].D-galactosegivenbyoralroutecausescognitiveimpairmentsinratswhichareaccompaniedbyoxidativedamage.Cognitiveimpairmentsisobservedintheopen-fieldtestinthe4thand6thweeksafterd-galadministration,aswellasanimpairmentinspatialmemoryintheradialmazetestafterthe6thweekofd-galadministration[3].PROTOCOLAnimalRats:D-galactoseisdissolvedinwaterforadministrationatthedoseof100mg/kgofbodyweight,andgivenAdministration[2][3]byoralgavage,onceaday,overaperiodof1,2,4,6or8weeks.Animalsarerandomizedintotwogroups:controlanimals(receivingwaterbyoralgavage)ord-galanimals(receivingD-galactosebyoralgavage).Thebehavioraltestsandbiochemicalanalysisareundertakenonthe1st,2nd,4th,6thand8thweeksafterthelastadministrationofd-gal[3].Mice:MaleadultC57BL/6micearerandomlydividedintothreegroups(control,D-galactose,andD-galactoseplusα-LA).D-galactose(100mg/kg)isinjectedsubcutaneously(s.c.)dailyintomicefor7weeks.α-LA(100mg/kgbodyweight)isinjectedperitoneally(i.p.)dailyconcomitantlyfor7weeks.Allcontrolanimalsaregivensaline[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE?SignalTransductTargetTher.2022Sep1;7(1):303.?PLoSBiol.2018Oct18;16(10):e2006483.?Cells.2022,11(20),3270.?IntJMolSci.2022Sep2;23(17):10045.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].CsiszovszkiZ,etal.StructureandfunctionoftheD-galactosenetworkinenterobacteria.MBio.2011Jun28;2(4):e00053-11.[2].CuiX,etal.ChronicsystemicD-galactoseexposureinducesmemoryloss,neurodegeneration,andoxidativedamageinmice:protectiveeffectsofR-alpha-lipoicacid.JNeurosciRes.2006Aug15;84(3):647-54.[3].BudniJ,etal.Oraladministrationofd-galactoseinducescognitiveimpairmentsandoxidativedamageinrats.BehavBrainRes.2016Apr1;302:35-43.M