Roche-LightCycler-480-中文操作說(shuō)明

Roche-LightCycler-480-中文

操作說(shuō)明（共20頁(yè)）-本頁(yè)僅作為預(yù)覽文檔封面，使用時(shí)請(qǐng)刪除本頁(yè)-

LightCycler?480中文操作說(shuō)明羅氏醫(yī)學(xué)儀器公司/應(yīng)用科學(xué)部門(mén)February2008#相對(duì)定量(RelativeQuantification).在選擇相對(duì)定量分析后，出現(xiàn)下面窗口:ReferenceSampleLociTionColorcoinpensntionrequiresmorethnnonefiltercombiiifitioiiCreatenjewanklysisReferenceSampleLociTionColorcoinpensntionrequiresmorethnnonefiltercombiiifitioiiWAntoPail口ColorCoinpeiisationWAntoPailReferenceSampleLocation:若Reference(Housekeepinggene)之樣品在同一盤(pán)上請(qǐng)選擇〃ln?Run〃，否則請(qǐng)選擇〃External〃可輸入另一次實(shí)驗(yàn)的數(shù)據(jù)一起分析。建議將AutoPair打勾讓計(jì)算機(jī)自動(dòng)將樣品配對(duì)。.進(jìn)入『Target』與『Reference』分頁(yè)，右下角顯示"Eff=2〃表示將目標(biāo)基因與參考基因的PCR效率以來(lái)計(jì)算。.若需要校正目標(biāo)基因與參考基因之PCR效率，則需要輸入標(biāo)準(zhǔn)曲線。在『Target』與『Reference』分頁(yè)之右下角，輸入(import)標(biāo)準(zhǔn)曲線。

Eff=2UseinrunUse?xteiHill...Eff=2UseinrunUse?xteiHill...SawasOHT?rnal...Useinrun：輸入(import)同一盤(pán)上之標(biāo)準(zhǔn)曲線。Useexternal：輸入(import)之前已儲(chǔ)存于數(shù)據(jù)庫(kù)中之標(biāo)準(zhǔn)曲線。.點(diǎn)選『Pairing』分頁(yè)，檢查計(jì)算機(jī)自動(dòng)配對(duì)結(jié)果是否正確。其中,紅色表示Unknown樣品，綠色表示Calibrator樣品。若欲外加配對(duì)，則可直接以鼠標(biāo)點(diǎn)選樣品后按〃Add〃即可增加配對(duì)。.點(diǎn)選『Results』分頁(yè)，點(diǎn)選〃Calculate〃即可得到計(jì)算結(jié)果。Ik國(guó)測(cè)jephui匕VirlualLiqMCfdw4匣3H4/Nd1Cimiii睡17md“7■仁恒gmax市 *口MuindiZwdifioraxliinfax:oichDUt益匚治力白Ik國(guó)測(cè)jephui匕VirlualLiqMCfdw4匣3H4/Nd1Cimiii睡17md“7■仁恒gmax市 *口MuindiZwdifioraxliinfax:oichDUt益匚治力白|InTormalloHSDhipc-hJfidcmiI!■￡￡j,R口工曰 Esip&Eilfftit2IXi,Eim,Targfel | ReftremceMHtki3呻帕乃津電Poe：S；31mlm。Na面。Cp^ncemraiiiJor?ncaihirar：uMorm日■時(shí)11jiiim^EzedlMliipIBcanUerv'ConectiaMriifian IRaliu RM。ST\Rilnn|atio§TO FmJ割rTarqatCu.1xOIL^CCb.1s^Eiattxe2a.07 0.02 4.39E-IReferencrGFIL*EEllbraDDi:mjrtl/El Targetrin-itn,J11,JIZSairpLc1丁 0.DZ 1.1TE-： 1.01 CK。。 VlEeCeteiurEUIljIEBangle133uT3Taruer口限立力H百，TTiSanfLuE32.5EE.SaE-3E.BfiE-1 Q.L2 O.flL Vig土口ED”讓日UIW“ 222.75111TargatDnlcnHIL^E&vt^Lb32^-22 Q,m 1.QGE-： 3.￡3 □.13 VIRefErencEU11L*35airpLe3凡U工ItlG/IlSTflrgetUiUm10.0ESairpL-=，anon i/i ?JIRrilDuanri三| Usie-r: 知嘯emadriiiriHi鐘1DOE-11K8J.1.COM：1.COE-2\1Apply[N喇四.數(shù)值和圖形輸出與絕對(duì)定量相同。(請(qǐng)參考絕對(duì)定量)基因分型(Genotyping)1.進(jìn)入基因分型的窗口中。IrHtmniMitVirtualLifMCydei4EO3fliFHatCmn^ededMyCotfn4pmL￥r(Trarf加琲4噴fiajiJyBjHREIi?B：nEitypinq王口uLn.P.unHb1LMtdlpfDg'mm!旺11541aCalazQnfmgaarLan:Ol￡,3匚3力自小工西2In-EnuIrHtmniMitVirtualLifMCydei4EO3fliFHatCmn^ededMyCotfn4pmL￥r(Trarf加琲4噴fiajiJyBjHREIi?B：nEitypinq王口uLn.P.unHb1LMtdlpfDg'mm!旺11541aCalazQnfmgaarLan:Ol￡,3匚3力自小工西2In-EnuIoRun.Belt抗壯F1F4OF3?FfiS-amMK■ItBelt;附白nd占ErtX5R=pLaPtIteLt8m■|X6R2P4口口亡KtLtStflr■|17Replu□￡KeltEtan19RetluofKeltStacHi由JxE>：!U>l!Ur>lHUTH5Repluo￡Hele8匚批d88①C)Z產(chǎn)府^10.1Eli11」的5.15?MelltiinqCaruesSc.liiFR料d均Gi?ikiIScore R的口0「ElfMITtfUE譚J"I,(Ih.NIH]YFltlsrCm*，C?lor3㈣磨屏0「. 呷「2.窗口右下角可設(shè)定分類(lèi)方式SaueasextAutoGroup:2.窗口右下角可設(shè)定分類(lèi)方式SaueasextAutoGroup:AutoGroup：自動(dòng)分組In-run：標(biāo)準(zhǔn)品包含在本次實(shí)驗(yàn)中External：標(biāo)準(zhǔn)品在之前已儲(chǔ)存之實(shí)驗(yàn)中3.點(diǎn)選“Calculate”即可得到分型結(jié)果。Tm分析(TmCalling).進(jìn)入Tm分析窗口守XfbiOEWSbltMiEitlum-1JDEHHislinr'EiitH口ndht-ilmii:il[iM-ihase:NvLsintula[IiiK?-ihle|三Usd：SifUcBAdniiJin-3tottlMOHHpt-BfcS：0OF1gl呻 rshHirimft*roi噓r皿也r咽?S?nHt3UdliBfhBb.■俎Me€?#rPw心nif||Thi1|th；■ PL 加>此1 OSMQ■ M hpl. e￡ ampLn 1 酯.弱■ m tetil- M L SS.S7■ PI €5.79N 也口人 CE SER1T 3 Q5.7Q■ OS FspL. M SspLfil ± q￡.q4| >?》能一字■ 典 Refiln rt SmLE 3 65.79■ 的電口人CE寺ERIE 3 。隊(duì)IH即pH7竺副舊疊⑥?一?一?&/FturCinth■_MuPadi

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MaxPeaks.(6orless).….選擇檢測(cè)模式EHyhProh&Hp Format j—RGr~IFormatI|SimpleProbe\即可得到每個(gè)樣品之Tm值。.點(diǎn)選“Calculate"即可得到每個(gè)樣品之Tm值。五.報(bào)告(Report)1.將分析后結(jié)果儲(chǔ)存后即可點(diǎn)選報(bào)告鍵。數(shù)據(jù)儲(chǔ)存報(bào)告.勾選想要呈現(xiàn)的報(bào)告內(nèi)容。.點(diǎn)選“Generate〃即可產(chǎn)生報(bào)告。.點(diǎn)選〃PDF〃即可將此報(bào)告儲(chǔ)存成.pdf檔案格式。。晡得#觸陽(yáng)?tompiftsr{Tr?e.ibhlU割i: Admin1210IS3HSSB?■RilhwiSmlimr命L：|ILL3ft>(iLit3[hMfirl-Viewfilial 昌昌目國(guó)〔工*4*曲］i|,M交？電.vjhP叫白廠dUMB0130-10Espeimenl0PralKiJ0Samples0In^rumeni:0RciacnHisi：町白圮GliSiLGVid匚電信百SetlhpaResutsSUI印IGB0a-:^kwnlaaonI*，'』LightCycler^4B0Software\ 1■■Rcporl一J；：：；：：：：' \ /。眼而疝小200&'i切下午■ 啟RfflC5上午ictus?口pe同加Osn&f卻隨mAdminE^dTin?3：O(30TT03:510313KH/1⑶T千CW3SGRun91”C師區(qū)過(guò)時(shí)ScrimeMerBiohLCEO120.?Macfo隔陋（hr即htacniaulusTerrijlal^R&cte儂g白?⑶彳PlaleDAm曲）隨20(假JI孫.[jpeiwislProqiamNanw-piG^inciiialianCjnsfeis1助d解啟Mod*Mo后Pio^ainiT即MlHold小knw,|P呻R也陋AcqusilKirKSkTaipalStepEisai叩口喉依附網(wǎng)Acqusilnn■Xrrvnc-nnd口nnnUuuitfflBs?旦⑹慟六.數(shù)據(jù)轉(zhuǎn)移至其它計(jì)算機(jī)（數(shù)據(jù)輸出與輸入）數(shù)據(jù)輸出L點(diǎn)選L點(diǎn)選(navigator).點(diǎn)選所要輸出的實(shí)驗(yàn)檔案，點(diǎn)選.而口「】即可將檔案輸出成Jxo檔案.將此ixo檔案