生物化學(xué)與分子生物學(xué)PPT圖片素材-Science_Slide

1、生物化學(xué)與分子生物學(xué)PPT素材,可根據(jù)需要選擇取舍,Metabolism of 5-fluorouracil (5-FU,5-Fluorouridine,5-FUMP,5-FdUMP,Uridine phosphorylase,5-Fluorodeoxyuridine,Thymidine phosphorylase,Orotatephosphoribosyltransferase,Thymidine kinase,5-FUTP,5-FdUTP,RNA,DNA,5-nucleotidase,5-nucleotidase,Uridine kinase,ATP,ADP,ATP,ADP,PRPP,We

2、stern Blotting,1. Resolve protein samples on SDS-PAGE,6. Incubate the blot with chemiluminescent HRP substrate and expose to film,2. Electrophoretically transfer fractionated proteins from gel onto PVDF membrane,3. Block the membrane with neutral protein (BSA or milk casein,4. Incubate the membrane

3、with primary antibody specific to target protein,5. Incubate the membrane with HRP-labeled secondary antibody specific to primary antibody,Cholesterol,Sterol Toolkit,Pregnenolon,Progesteron,Aldosteron,Cortisol,Corticosterone,Androstendione,Testosteron,Estriol,17-Estradiol,1, 25-Dihydroxycholecalcife

4、rol,Cholic acid,Covalent modification of proteinsPoly(ADP ribosyl) Polymerase (PARP,Poly(ADP ribosyl)polymerase(PARP,NAD,Nicotinamide,Poly(ADP ribosyl)polymerase(PARP,Poly(ADP ribosyl)chain elongationand branching,Nicotinamide,NAD,NAD,DNA polymerase,DNA polymerase,DNA polymerase,Oligosacharyltransfe

5、rase,N-glycosylation pathway in ER,Mann-P-Dolichol transferases,Flippase,Dolichol,P,P,Dolichol,P,P,Dolichol,P,P,Dolichol,P,P,GlcNAc-Ptransferase,GlcNActransferase,Dolichol,P,GDP-Mannosyl transferases,Glc-P-Dolicholtransferases,Dolichol- pyrophofphatase,Cytosol,ER,40S,60S,UTP,CTP,TMP,Pyrimidine Nucle

6、otide Interconversions,UMP,ATP,ADP,H2O,UDP,Nucleosidemonophosphatekinase,ATP,ADP,Nucleosidediphosphatekinase,ATP,ADP,CTPsynthase,Ribonucleotidereductase,deoxyUDP,Nucleosidediphosphatephosphohydrolase,deoxyUMP,Thymidylatesynthase,N5,N10-MethyleneTHF,DHF,TTP,Nucleosidemonophosphatekinase,Nucleosidedip

7、hosphatekinase,Myo-Inositol-3-phosphateIns(3)P1,Biosynthesis and metabolism of phosphoinositides,Glucose 6-pgosphate,Ins(3)P1synthase,Ins(3)P1phosphatase,Myo-InositolIns,PtdInssynthase,PtdIns,Myo-inositolkinase,PtdIns-4-P,PtdIns4-kinase,PtdIns5-kinase,PtdIns-4,5-P2,PI3K,PtdIns-3,4,5-P3,PLC,PLC,PI3K,

8、PtdIns-3,4-P2,Ins-1,3,4-P3,Ins-1,3,4,5-P4,Ins-1,4,5-P3,PLC,PTEN,PTEN,Ins-1,4-P3,PTEN,PTEN,Fatty acidsynthase,ketoacylACP-synthase,Fatty acids synthesis,Acetyl CoAcarboxylase,ATP,ADP + Pi,Acetyl CoA,Malonyl CoA,Citrate,Oxaloacetate,ATP,ADP + Pi,Citrate lyase,Acetyltransacylase,Malonyltransacylase,ket

9、oacylACP-reductase,hydroxyacylACP-dehydratase,Enoyl-ACPreductase,Pantetheine,NADPH,NADP,NADPH,NADP,Cholesterol de novo synthesis IIsoprenyl formation,Acetoacetyl-CoA,Thiolase,HMG-CoAsynthase,Mevalonate,3-Hydroxy-3-methylglutaryl-CoA,Mevalonatekinase,Phosphomevalonatekinase,Mevalonate-5-pyrophosphode

10、carboxylase,5-Phosphomevalonate,5-Pyrophosphomevalonate,3-Isopentenylpyrophosphate,3, 3-Dimethylalylpyrophosphate,Isomerase,HMG-CoAreductase,2 NADPH,2 NADP,ATP,ADP,ADP,ATP,ADP,ATP,Cholesterol,Biosynthesis of bile acids,7-Hydroxycholesterol,Sterol 7hydroxylaseCYP7A1,7-Hydroxy-4-cholesten-3-one,3-Hydr

11、oxystroid dehydrogenaseHSD3B7,7, 12-Dihydroxy-4-cholesten-3-one,Sterol-12-hydroxylaseCYP8B1,5-Cholestan-3, 7, 12-Triol,Oxosteroid-5-reductaseAKR1D1,Sterol 27-hydroxylaseCYP27A1,3, 7, 12-Trihydroxy-5-cholestanoic acid,AKR1D1AKR1C4,5-Cholestan-3, 7-diol,12-Deoxycholic acid,3-Hydroxy-steroid dehydrogen

12、aseAKR1C4,Biosynthesis of RNA,RNAPolymerase,Biosynthesis of DNA,DNAPolymerase,oxidation,Mitochondrial matrix,Intermembranespace,Palmitoyl CoA,Palmitoyl CoA,Trans 2 enolopalmitoyl CoA,3-Hydroxypalmitoyl CoA,3-Ketopalmitoyl CoA,Myristoyl CoA,Acetyl-CoA,Palmitoyl CoA,Palmitoyl CoAligase,ATP,AMP,Carniti

13、neacyltransferase I,Carnitineacyltransferase II,Acyl CoAdehydrogenase,Enoyl CoAhydratase,3-Hydroxyacyl CoAdehydrogenase,Citric AcidCycle,FAD,FADH2,NAD,NADH,Cytoplasm,H2O,Myo-Inositol-3-phosphateIns(3)P1,Phospho-myo-inositol Toolkit 2,Myo-Inositol-3,4-diphosphateIns(3,4)P2,Myo-Inositol-3,6-diphosphat

14、eIns(3,6)P2,Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3,Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3,Myo-Inositol-3,4,5,6-tetraphosphateIns(3,4,5,6)P4,Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3,Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4,Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4,Myo-I

15、nositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4,Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5,Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1,3,4,5,6)P5,Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5,Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5,Myo-Inositol-1,2,3,4,5,6-hexaphosphateI

16、ns(1,2,3,4,5,6)P6 (Phytic acid,Myo-Inositol-3-phosphateIns(3)P1,Phospho-myo-inositol Toolkit,Myo-Inositol-3,4-diphosphateIns(3,4)P2,Myo-Inositol-3,6-diphosphateIns(3,6)P2,Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3,Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3,Myo-Inositol-3,4,5,6-tetraphosphateIns(3

17、,4,5,6)P4,Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3,Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4,Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4,Myo-Inositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4,Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5,Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1

18、,3,4,5,6)P5,Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5,Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5,Myo-Inositol-1,2,3,4,5,6-hexaphosphateIns(1,2,3,4,5,6)P6 (Phytic acid,Glycogen,Glycogenin,8-oxoguanine,A common DNA lesions Toolkit I,O6-methylguanine,Uracil,Thymine glycol,1, N6-e

19、thenoadenine,3-methyladenine,Cyclobutane pyrimidine dimers,Photoadduct,Ribose,Glucuronate,Arabinose,Fructose,Glucose,Galactose,Mannose,Fucose,Sialic Acid,Inositol,D-Abequose,L-Rhamnose,A carbohydrate toolkit,PGH2,TXA2,TXB2,Bioactive lipids - Prostanoids,PGD2,PGJ2,PGE2,PGA2,PGB2,PGF2,PGF-M,PGI2,6-ket

20、o-PGF1,Bioactive lipids - Leukotrienes,5-HETE,LTA4,LTB4,LTC4,LTD4,LTE4,20-OH- LTE4,LXA4,LTB4,LTF4,LXA,LXB,Bioactive lipids,Arachidonate,Ceramide,Sphingosine-1-phosphate,PAF,Phosphatidic acid,Anandamide,2-Arachidonoylglycerol,qGTP,NO mediated attenuation of adrenergic Gq signaling in VSMC,q GDP,PLC,q

21、GTP,Ca,sGC,ANP,PKG,PKC,MLCK,myosin,myosin,myosin,P,P,CaM,MLCK,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,K,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,CaM,Ca,PA,1,4,5-Ins,P,SGTP,RGS function and regulation,S GDP,AC,PKA,cAMP,cAMP,cAMP,cAMP,cAMP,cAMP,cAMP,AC,SGTP,Ligand,Ligand,P,P,cAMP,cAM

22、P,cAMP,TNF,Modular Structure of Cytokine Receptors,IFNR2515 aa,IFNR1 530 aa,IL-6R 449 aa,IL-11R 400 aa,IL-10R1 557 aa,IL-10R2325 aa,STATbinding,IL-13R1 427 aa,IL-4R 825 aa,FN III,FN III,CCCC,WS-WS,FN III,FN III,FN III,FN III,FN III,FN III,FN III,FN III,FN III,FN III,SOCS,gp130 896 aa,STATbinding,Tyk

23、2,Jak1,INFR1 489 aa,STATbinding,FN III,FN III,Jak1,IL-22R1 574 aa,STATbinding,FN III,FN III,Jak1,Jak1,INFR2 332 aa,FN III,FN III,Jak2,STATbinding,Tyk2,Jak1,Jak2,Jak1,IL-13R2 380 aa,FN III,FN III,CCCC,WS-WS,FN III,FN III,CCCC,WS-WS,FN III,FN III,FN III,STATbinding,STATbinding,Ig,Ig,Ig,Ig,OSM-R952 aa,

24、Jak3,FN III,FN III,FN III,FN III,STATbinding,Ig,FN III,FN III,CCCC,WS-WS,FN III,FN III,CCCC,WS-WS,FN III,FN III,CCCC,WS-WS,LIF-R 1053 aa,Jak3,FN III,FN III,FN III,STATbinding,Ig,FN III,WS-WS,FN III,FN III,CCCC,WS-WS,STATbinding,STATbinding,SHP,FN III,CCCC,STATbinding,STATbinding,Tyk2,FN III,FN III,C

25、CCC,WS-WS,Ig,SOCS,SHP,FN III,FN III,CCCC,WS-WS,Ig,Jak3,c 349 aa,IRS,FN III,FN III,CCCC,WS-WS,Ig,Jak3,FN III,FN III,FN III,FN III,FN III,CCCC,WS-WS,IL-12 662 aa,FN III,WS-WS,SOCS,IRS,STATbinding,Ig,Tyk2,Fibronectintype III domain,Ig - like,IRS-binding,Fibronectintype III domainwith cysteines,SOCS-bin

26、ding,STAT-binding,JAK/TYK-binding,Fibronectintype III domainwith WSXWS,SHP-binding,SHP,Adaptor proteins - selected (human,SAM domain,Src-homology 3 domain,SH3,SH2,Src-homology 2 domain,GRB2,PTBdomain,PTB,Cysteine-richregion 1,Calponinhomology domain,C-1,Calponin,Sprouty,Sprouty domain,SLAP,Crk,GADS

27、(GRID,Nck1,BLNK,Rho-GEF,Rho-GEF homologydomain,Vav-1,SLP-76,SHC-1,APS,SAM,PH,Plekstrin homologydomain,Protein separation using SDS-PAGE(Laemmli system,Stackinggel,Resolvinggel,1. Apply protein/dye samples into polyacrylamide gel wells,2. Run the electrophoresis until dye reaches the end of the gel,3

28、. Remove the gel from the apparatus and stain for proteins,A Column Chromatography Toolkit,Embryonic stem cells,Egg donor,Sperm donor,Egg fertilization,Culture fertilized cells forseveral cell divisions,Dissociate cell clusterfor subculturing andfurther utilization,Freeze in N2 forlong-term storage,

29、Use for In Vitrofertilization,Potential use fortherapeutic cloning,Common Cloning Vectors,Metabolism of capecitabine,5-FU,5-Fluorouridine,5-FUMP,5-FdUMP,Uridine phosphorylase,5-Fluorodeoxyuridine,Thymidine phosphorylase,Orotatephosphoribosyltransferase,Thymidine kinase,5-FUTP,5-FdUTP,RNADNA,5-nucleo

30、tidase,5-nucleotidase,Uridine kinase,ATP,ADP,ATP,ADP,PRPP,CapecitabineXeloda(prodrug,Carboxyl-esterase,5-Fluoro-5-deoxycytidine,Cytidinedeaminase,Thymidine phosphorylase,5-Fluoro-5-deoxyuridine,Pharmacologicallyactive compound,Pertussistoxin,NAD,ADP,Choleratoxin,AC,ATP,cAMP,AC,SGTP,Ligand,Pharmacolo

31、gical modulation of cAMP,Ligand,Ligand,Ligand,NAD,ADP,Forskolin,Rolipram,AMP,PDE,Mechanism ofPenicillin(inhibition of crosslinkingpeptidoglycan,Tetra-peptide,Penta-glycine,Glycopeptide transpeptidase,Penicillin,40S,Inhibition of translation byRapamycin (Sirolimus,Growth Factor Receptors,IP3K,PDK,PKB

32、,mTOR,40S,30S,eIF4G,eIF4E,PABP,eIF4A,4E-BP1,eIF4E,P,P,4E-BP1,P,P,P,P,P,S6K,P,P,P,P,P,Ribosomalproteins,eEF2,eEF2,P,P,40S,30S,eEF2kinase,CaM,eIF2,Rapamycin,FKBP-12,TMP,Mechanism of 5-fluorouracil (5-FU,deoxyUMP,Thymidylatesynthase,TTP,DNA synthesis,5-Fluorouridine,5-FUMP,5-FdUMP,Uridine phosphorylase

33、,Uridine kinase,5-Fluorodeoxyuridine,Thymidine phosphorylase,Orotatephosphoribosyltransferase,Thymidine kinase,N5,N10-methylene-THF,DHF,The Life cycle of Plasmodium spp,Bloodvessel,Endothelium,Hepatocytes,Kupffer cells,Merozoite Sporozoite Trophozoite Schizont Macrogametocyte Microgametocyte,1,2,3,4,Oocyst Gametogenesis,The Life cycle of HeartwormDirofilaria immitis,Microfilariae,Larvae,14 days