BiotechMarine時(shí)光干細(xì)胞ppt課件

1、光陰干細(xì)胞光陰干細(xì)胞CellulosomesCellulosomes Eryngium Eryngium 海洋活性成分2021/11/022021/11/02 -濱海刺芹濱海刺芹2植物選取-高鹽分海洋植物植物選取-高鹽分海洋植物鹽生植物與淡生植物的區(qū)別 Salty soil 含鹽份土壤 Marine wind spray aggressions 海風(fēng)的侵襲 相應(yīng)的儲(chǔ)水系統(tǒng) (葉, 莖) 離子管理系統(tǒng)植物選取-高鹽分海洋植物UNIQUE PROPERTIES OF HALOPHYTE PLANTS:高鹽份植物的獨(dú)特特性:Specific molecules 特殊物質(zhì): Lipids 脂質(zhì)Amin

2、o-acids such as proline 氨基酸例如脯氨酸Polyols 多元醇Polyphenols 多酚Soluble sugars 可溶性糖Specific properties 獨(dú)特特性:超強(qiáng)的抵御自在基破壞抗炎癥特性5濱海刺芹的故事 法國(guó)布列塔尼海岸的Vauville地域處于兩大對(duì)立生態(tài)系統(tǒng)北大西洋冰冷氣候和南大西洋溫潤(rùn)氣候的交界處，此地獨(dú)特的微氣候環(huán)境使得動(dòng)植物種群如此繁茂。這種生物地理區(qū)域間的重疊極大地提升了生物多樣性，刺激了兩大生態(tài)區(qū)域眾多有代表性生物種群的繁衍生長(zhǎng)。 濱海剌芹是在沿海生長(zhǎng)的，它的根基延伸至海底深處，而頂部那么生長(zhǎng)至海平面以上。它的根部構(gòu)造會(huì)由于遭到外界繼

3、續(xù)不斷的壓力而被迫順應(yīng)，生命力變得非常頑強(qiáng)。干細(xì)胞消費(fèi)工藝 消費(fèi)工藝Stem cell* culture 干細(xì)胞培育Stem cell production in suspension 懸浮液培育干細(xì)胞Filtration from the aqueous medium 以水為媒介提取干細(xì)胞Lyophilisation(Freeze dry) 冰凍枯燥法Bioreactor 生物反響器 混合生長(zhǎng)因子,礦物質(zhì),微量元素We take an explant from meristematic area 從分生組織提取外植體干細(xì)胞優(yōu)勢(shì)基于干細(xì)胞新技術(shù)的優(yōu)勢(shì) 各分化細(xì)胞含有植物的成分 產(chǎn)品的特性是最最

4、優(yōu)化與最大化 更新-恢復(fù)活力 仿生性與皮膚結(jié)合“滿活力的后代,生命的精華 無(wú)污染-無(wú)溶劑-無(wú)防腐劑-可繼續(xù)開(kāi)發(fā)與資源的維護(hù) 無(wú)細(xì)胞毒性9干細(xì)胞專利和ECOCERT證書已獲美國(guó)專利已獲法國(guó)專利已獲ECOCERT認(rèn)證 CTFA/INCI Name: Eryngium Maritimum Callus culture Filtrate 海洋刺芹干細(xì)胞 CAS N : 90028-44-7EINECS/ELINCS N : 289-835-1建議運(yùn)用量: 0.05%外觀: powder 粉末顏色: beige 淡棕色氣味: none 無(wú)氣味溶解性: water dispersible 水分散防腐劑:

5、none 無(wú)防腐劑認(rèn)證: Ecocert 經(jīng)過(guò)生態(tài)認(rèn)證Cellulosomes Eryngium 特性Cellulosomes Eryngium 處理方法Induction of stem cells from Eryngium maritimum.從海洋刺芹中提取干細(xì)胞Sea plants are the first & original organisms to have come out of the water. 海洋植物是從海洋中出現(xiàn)的最原始的有機(jī)體Sea plants, with their roots in the marine original medium have, at

6、the origin, to face the constraints of the terrestrial conditions (dehydration - light - UV- and additional stress from salt) 海洋植物的根部存在于海洋中,需求面對(duì)環(huán)境的各種壓力(缺水-光照-UV和額外的高鹽份) They developed an unique answer in the adaptation phenomenon to the terrestrial environmental conditions.他們面對(duì)如此惡劣的環(huán)境構(gòu)成了本人獨(dú)特的特性FAMIL

7、Y: APIACAE 傘形花科 PROTECTED SPECIES 受維護(hù)物種LOCATED ON DUNE AND SHINGLE SEA SHORE 坐落在沙丘和碎石海邊Physiological phenomena occurring at all skin levels在皮膚各層出現(xiàn)的生理景象EPIDERMIS 表皮 表皮變薄,不規(guī)那么和紊亂 表皮層改動(dòng) (形狀和功能)DERMO-EPIDERMAL JUNCTION 表皮與真皮銜接層 表皮真皮銜接層變得扁平 細(xì)胞間有效銜接減少,出現(xiàn)紊亂DERMIS 真皮 真皮層厚度減少 Extra Cellular Matrix (ECM) 細(xì)胞外基

8、質(zhì)遭到破壞 (膠原蛋白, 氨基脯聚糖) Less fibroblasts & disorganized elastic fibers 缺乏成纖細(xì)胞和彈性縣委紊亂目的表皮真皮銜接層,皮膚的構(gòu)造Integrins B1 整聯(lián)蛋白B1: 將細(xì)胞外基質(zhì)同細(xì)胞內(nèi)的骨架網(wǎng)絡(luò)連成一個(gè)整體并把細(xì)胞外信號(hào)向細(xì)胞內(nèi)傳送的作用 Laminin 5 層粘連蛋白5: 層粘連蛋白5經(jīng)過(guò)受體介導(dǎo)，具有促進(jìn)上皮細(xì)胞粘附、遷移和分散的功能 Elastin 彈性蛋白: 支持皮膚彈性Fibulin 5 纖蛋白5: 廣泛分布于富含彈性蛋白的組織，能直接與原彈性蛋白結(jié)合,加強(qiáng)彈性蛋白/整聯(lián)蛋白之間的內(nèi)部銜接和細(xì)胞外基質(zhì)的彈性纖維的構(gòu)造

9、Fibrillin 原纖維蛋白: 可以合成纖維蛋白Collagens IV & VII : 加強(qiáng)基底層的構(gòu)造和溝通表皮真皮銜接層總結(jié)ACTIONS ON EPIDERMIS 表皮層作用調(diào)整角質(zhì)生成增生+分化ACTIONS ON DERMO-EPIDERMAL JUNCTION維護(hù)與刺激表皮真皮銜接層ACTIONS ON DERMIS 真皮層作用刺激細(xì)胞外基質(zhì)成分維護(hù)細(xì)胞外基質(zhì)成分PROTECTION 維護(hù)抵御自在基的破壞減少炎癥Evaluation of Cellulosomes Eryngium on mediators and molecules involved on communica

10、tion and junction between epidermis and dermis 評(píng)價(jià)Cellulosomes Eryngium 對(duì)于表皮和真皮層間銜接和溝通的分子與媒介的作用 without irradiation 無(wú)照射 under irradiation UVA (10 J/cm2) + UVB (150 mJ/cm2) 在UVA和UVB下照射(immunohistochimical study on reconstitued epidermis 在人造表皮上進(jìn)展免疫組化研討) Markers analysed:Integrin 3 B1 整聯(lián)蛋白- Laminins 5 層

11、粘蛋白5- Elastin 纖維蛋白Fibulin 5 纖維蛋白5 Fibrillin 纖維原蛋白- Collagens 膠原蛋白表皮真皮銜接層Without irradiation 無(wú)照射MARKERS STIMULATED Integrins B1 Laminin 5 Elastin Fibulin 5 Fibrillin Collagens IV et VIIAdherence & transduction of the matrix and cellsConsolidation of the construction of the adherent structure of hemid

12、esmosomes.Support the skin elasticityReinforce the interconection between elastin / integrins and the organisation of the elastic fibers in the ECMOrientation of the elastic fibersReinforcement of the anchorage fibres et the organisation of the architecture of the basal layer and its communication結(jié)果

13、1刺激表皮真皮銜接層,基底層和真皮上層的大分子合成ACTIONSUnder irradiation UVA (10 J/cm2) + UVB (150 mJ/cm2) 在UVA和UVB照射下(層粘連蛋白5和纖蛋白5除外)MARKERS STIMULATED Integrins B1 Elastin Fibrillin Collagens IV et VIIACTIONSAdherence of the matrix and cellsSupport the skin elasticityOrientation of the elastic fibersReinforcement of the

14、anchorage fibres et the organisation of the architecture of the basal layer and its communication結(jié)果2Cellulosomes Eryngium 對(duì)處于真皮表皮銜接層，基底層和真皮上部大分子的神經(jīng)合成油積極的調(diào)理作用Regenerating activity on skin 皮膚更新作用Proliferation ( KI67) 繁衍Differentiation (K10 & Involucrin 苞蛋白) 分化Protection against 維護(hù)Free Radicals (MDA me

15、asurement) 抗自在基Inflammatory activity (IL1 - IL6 & PGE2) 抗炎癥表皮整體維護(hù)效果/體外測(cè)試皮膚更新Application of a cream at 0.05% on reconstitued epidermis (72H): 運(yùn)用含量為0.05%的膏霜于人造表皮(72小時(shí)): Proliferation of the new keratinocytes from the basal layer 促進(jìn)基底層新角化細(xì)胞的生成immunohistochemical treatment to measure KI 67(mitotic index

16、) 免疫組織化學(xué)處置來(lái)測(cè)試KI67 -促進(jìn)細(xì)胞生成和皮膚更新Increase by +19%C皮膚更新運(yùn)用含有0.05%光陰干細(xì)胞的膏霜于表皮 (72H):在顆粒層出現(xiàn)分化標(biāo)志K10和苞蛋白效果: 加強(qiáng)促進(jìn)細(xì)胞生長(zhǎng)與分化,使肌膚更紅潤(rùn)光澤ControlInvolucrineK10With Cellulosomes E調(diào)整角質(zhì)生成 免疫組化標(biāo)志HB-EGF :(肝素結(jié)合性表皮生長(zhǎng)因子)Cellulosomes Eryngyium 促進(jìn)人肝素結(jié)合性表皮生長(zhǎng)因子的構(gòu)成Acid vitamin A 維生素A和維生素A酸均有促進(jìn)生長(zhǎng)的作用，并能使上皮堅(jiān)持正常的作用 ,表皮角質(zhì)構(gòu)成細(xì)胞,黑色素細(xì)胞及真皮成

17、纖維細(xì)胞都是維A酸作用重要的靶細(xì)胞 treated skin with Cell. EryngiumUntreated skinHB-EGFIN VITRO TEST ON DERMIS COMPONENTS 對(duì)真皮組成部分的體外測(cè)試 Stimulation of the collagen 促進(jìn)膠原蛋白合成 Stimulation of the proteoglycans 促進(jìn)蛋白聚糖合成membrane (peri & trans) 膜Matrice 矩陣真皮標(biāo)志物 - Matricial proteoglycans* (biglycan 二聚糖, decorin中心蛋白多糖)- Trans

18、membranar proteoglycans (syndecan) 跨膜蛋白聚糖- Perimembranar proteoglycans 前膜蛋白聚糖 - Collagen 膠原蛋白促進(jìn)細(xì)胞外基質(zhì)合成proteoglycans perimembraneproteoglycans transmembraneproteoglycans matricecollagenControl/0.05% Cell. Eryngium33 %40 %38 %37 %促進(jìn)蛋白多聚糖和膠原蛋白的生成* receptor and 抗自在基方法 & 資料:Epidermis on culture 培育表皮細(xì)胞MDA

19、 measurement after 24 hours in contact 24小時(shí)后測(cè)試MDA含量MDA was extracted then measured by HPLC (fluorescence) MDA提取出來(lái),運(yùn)用高性能液體色譜測(cè)試Lipoperoxidation initiated by UVB (150mJ/cm)抑制由UVB引導(dǎo)產(chǎn)生的自在基MDA: Malondialdehyde, final by-product and tracer of lipoperoxidationMDA (M/mg protein)%Control683 25-Positive contro

20、l UVB 938 52+37Cream + 0.05% CEM + UVB630 49-33Cream + 0.25 nM Vit.E + UVB585 37-Cellulosomes Eryngium 光陰干細(xì)胞強(qiáng)效抑制角質(zhì)構(gòu)成細(xì)胞在壓力的情況下活化-在生理?xiàng)l件下沒(méi)有任何的變化減少由UVB引起的炎癥和刺激 under UVB exposure (150 mJ/cm2)UVB & 0.05% Cell. Eryngiumvariation in %IL1178 10126 12- 29IL6103 774 9- 28PGE2154 11105 12- 32Protocol & materia

21、l:Human Keratinocytes on culture 培育的人體角化細(xì)胞Cytokines measurement under physiological conditions and UVB exposure在生理學(xué)條件和UVB照射下測(cè)試細(xì)胞活素體內(nèi)測(cè)試運(yùn)用28天年齡在35到60歲的20位志愿者,皮膚都顯現(xiàn)松弛一天運(yùn)用兩次含有0.05%光陰干細(xì)胞的膏霜由皮膚病學(xué)家評(píng)價(jià)Clinical evaluation 醫(yī)學(xué)評(píng)價(jià)0-28天,皮膚病學(xué)家衡量不同的臉部(面頰.額頭,鼻子,下巴)臨床特征(紅斑,枯燥,粗糙,脫皮,角質(zhì)角質(zhì)化,浮腫等) 運(yùn)用后, 皮膚科醫(yī)生發(fā)生下面有明顯的改善:Erythematic stage 紅斑程度Dryness stage 枯燥程度 DoD28Variation in %SignificativitySkin tightening4,15,227%p=0,002*Skin smoothness4,255,8538%p=0,001*Hy