紅景天苷作用機(jī)制 - Medchemexpress - MCE中國(guó)

1、Product Data SheetSalidrosideCat. No.: HY-N0109CAS No.: 10338-51-9分式: CHO分量: 300.3作靶點(diǎn): mTOR; Apoptosis作通路: PI3K/Akt/mTOR; Apoptosis儲(chǔ)存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect from light)溶解性數(shù)據(jù)體外實(shí)驗(yàn) H2O : 100 mg/mL (333.00 mM)* means soluble, but saturation unknown.

2、SolventMass1 mg 5 mg 10 mgConcentration制備儲(chǔ)備液1 mM 3.3300 mL 16.6500 mL 33.3000 mL5 mM 0.6660 mL 3.3300 mL 6.6600 mL10 mM 0.3330 mL 1.6650 mL 3.3300 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存式和期限：-80C, 6 months; -20C, 1 month (protect from light)。-80C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)內(nèi)使，-20C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)內(nèi)使

3、。BIOLOGICAL ACTIVITY物活性 Salidroside種脯氨酰內(nèi)肽酶 (prolyl endopeptidase) 抑制劑。Salidroside 可通過(guò)激活 mTOR 信號(hào)緩解腫瘤惡病質(zhì)模型中的惡病質(zhì)癥狀。Salidroside 還能通過(guò)增強(qiáng) PINK1/Parkin 介導(dǎo)的線(xiàn)粒體噬來(lái)保護(hù)多巴胺能神經(jīng)元。IC & Target mTOR體外研究Salidroside (100 M) inhibits prolyl endopeptidase (PEP) activity (10.61.9%). Prolyl endopeptidase is an enzyme thatpla

4、ys a role in the metabolism of proline-containing neuropeptidase which is recognized to be involved in learningand memory1. Salidroside, one of the major phenylpropanoid glycosides found in R. rosea L, is consumed almostdaily as a nutritional supplement in many countries and has been identified poss

5、essing potential anti-fatigue andanoxia,anti-aging, and anti-Alzheimers disease activities. Salidroside can improve muscle nutrition via increasingmTOR, p-mTOR, and MyHC expression2. SH-SY5Y cells are exposed to 0-600 M MPP+ for 12-48 h and the resultsshow that MPP+ results in a significant decrease

6、 of cell viability in a concentration and time-dependent manner. Cellsare pretreated with 25-100 M Salidroside (Sal) for 24 h and then exposed to 500 M MPP+ for an additional 24 h.Page 1 of 2 www.MedChemESalidroside concentration-dependently prevents MPP+-induced decrease of cell viability. Annexin

7、V/PI staining is acommon method for the detection of apoptotic cell. Salidroside significantly decreases the number of Annexin V/PI-stained cells treated by MPP+ which is in a concentration-dependent manner. Apoptotic cell could also bemorphologically evaluated by Hoechst staining. In Hoechst staini

8、ng, apoptotic cells are characterized by reducednuclear size, chromatin condensation, intense fluorescence, and nuclear fragmentation. Salidroside notably inhibitsMPP+-induced increase of chromatin condensation, intense fluorescence, and nuclear fragmentation in SH-SY5Y cells3.體內(nèi)研究 Salidroside is a

9、natural antioxidant extracted from medicinal food plant Rhodiola rosea. Salidroside (100 mg/kg/day)shows strong glucose lowering effect on db/db mice which is similar to effect of Metformin (200 mg/kg/day). For thisreason, the dose of 100 mg/kg/day salidroside is used4.PROTOCOLCell Assay 3 SH-SY5Y c

10、ells are seeded in 96-well plates at 1104 cells per well. After the treatment with Salidroside (25-100 M)and MPP+, cell viability is measured by MTT assay. Briefly, cells are incubated with 500 g/mL MTT at 37C for 4 h.After that, the medium is removed and 150 L DMSO is added and shaking is conducted

11、 for 10 min. Absorbance ismeasured at 570 nm in a microplate reader and the results are expressed as folds of control3.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice4Administration 4 The 4-week-old male C57BL/6 mice are fed a high-fat diet

12、(HFD) (n=16) or normal chow diet (n=8). After 10 weeks ofthe HFD, salidroside intervention (100 mg/kg/day) is initiated by gavage once a day for 5 weeks. The control groupsare given vehicle (saline). The 4-week-old male C57Bl/KsJ (BKS) mice (wild type, n=8) and BKS.Cg-Dock7m +/+Leprdb/J (db/db) mice

13、 (n=16) are used. Salidroside (100 mg/kg/day) is administrated orally by gavage once a day for5 weeks. The control groups are given vehicle (saline). Fasting blood glucose and body weight of mice are monitoredevery 5 days. Glucose measurements are performed on blood drawn from the tail vein using a

14、Glucometer.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶(hù)使本產(chǎn)品發(fā)表的科研獻(xiàn) Invest Ophthalmol Vis Sci. 2019 May 1;60(6):2072-2082. Int J Oncol. 2019 Jun;54(6):1969-1980. Int Immunopharmacol. 2020 Feb.See more customer validations on HYPERLINK www.MedChemE ww

15、w.MedChemEREFERENCES1. Fan W, et al. Prolyl endopeptidase inhibitors from the underground part of Rhodiola sachalinensis. Chem Pharm Bull (Tokyo). 2001 Apr;49(4):396-401.2. Chen X, et al. Salidroside alleviates cachexia symptoms in mouse models of cancer cachexia via activatingmTOR signalling. J Cac

16、hexia Sarcopenia Muscle.2016 May;7(2):225-32.3. Wu L, et al. Salidroside Protects against MPP+-Induced Neuronal Injury through DJ-1-Nrf2 Antioxidant Pathway. Evid Based Complement Alternat Med.2017;2017:5398542.4. Ju L, et al. Salidroside, A Natural Antioxidant, Improves -Cell Survival and Function via Activating AMPK Pathway. Front Pharmacol. 2017 Oct 18;8:749.Page 2 of 3 www.MedChemE5. Li R, et al. Salidroside Protects Dopaminergic Neurons by Enhancing PINK1/Parkin-Mediated Mitophagy. Oxid Med Cell Lo