PHTPP-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPHTPPCat. No.: HY-103456CAS No.: 805239-56-9分式: CHFNO分量: 423.31作靶點(diǎn): Estrogen Receptor/ERR作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 25 mg/mL (59.06 mM; Need ultrasonic)H2O :

2、 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.91 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (5.91 mM); Clear solution1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEBIOLOGICAL ACTIVITY物活性 PHTPP種選擇性的 ER 拮抗劑。IC50 & Target ER 1體外研究 PHTPP is a selective ER

3、antagonist. PHTPP reduces FSH-mediated cAMP production by 80% (p 1.PHTPP (10-6 M) inhibits E2-stimulated ER activity, but does not suppress E2-stimulated ER activity. Ahigh dose of PHTPP (10-6 M) slightly increases class 1 Igf1 mRNA expression, and facilitates the DPN-induced increase in class 1 Igf

4、1 mRNA expression 2.PROTOCOLCell Assay 1 Cells are plated at a density of 3 to 4105 in 1 mL of medium per well in a 24-well plate for cell viability andcAMP analysis. Cells are cultured in Dulbeccos Modified Eagle Medium with PHTPP (1 M), or ethanol(0.1%) as the vehicle. The incubator is set to an a

5、tmosphere of 5% CO2 in air at 37C, and cultures areallowed to acclimate for at least 24 h. The culture medium is then aspirated and replaced with serum-freeDMEM/F12 containing 0.1 M androstenedione. The cells are collected for intracellular cAMP and to test cellviability 1.MCE has not independently

6、confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) J Agr Food Chem. 2019 Apr.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Compton DR, et al. Pyrazolo1,5-apyrimidines: estrogen receptor ligands possessing estrogen receptor beta antagonist activit

7、y. J MedChem. 2004 Nov 18;47(24):5872-93.2. Ogo Y, et al. IGF-1 gene expression is differentially regulated by estrogen receptors and in mouse endometrial stromal cells andovarian granulosa cells. J Reprod Dev. 2014;60(3):216-23. Epub 2014 Mar 25.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use