數(shù)字pcr與同類產(chǎn)品的比較

SuperiorValuePropositionforRainDrop?DigitalPCR

Partitions10,000,00020,00020,0003,072Multiplexing10-plex2-plex2-plex2-plexDynamicrange5-6logs>3logs>3logs3logsSensitivity1in250,0001in6,000?1in1,000Closed-tubeSystemYesNoYesYesWorkflowProprietaryReagentsNoYesYesYesThroughput/Shift2419218?12Instrumentprice$225,000$19,000$90,000$170,000Pricepersample$30$3$15-$35$150QuantStudioOpenArrayQX100QuantStudio3DQX100?DropletDigital?PCRSystem2LoadsamplesandoilintocartridgeAttachsealinggasketPlacecartridgeintoGeneratorTransfersamplesintoplatePipettedropletsfromcartridgeRemovecartridgefromGeneratorandremovesealinggasketSealplateRemoveplatefromcyclerLoadintoReaderandrunAnalyzedatausingQuantaSoftTotaltime~6hours36minsSellingAgainstBioRadWorkflow3CompetitorRainDropSolutionTakeHome20,000dropletsgeneratedUpto10,000,000dropletsgenerated20Kdropletsdon’tgiveyousinglemoleculecounting–relianceonPoissonstatisticstogenerateresults.Limitedtoduplexing.CrosscontaminationofsamplesfromreusedsamplingprobeandflowpathSingleusedisposableflowpathYouworkhardtogetthehighestqualityresults…don’triskthissample’sanswerwiththeprioroneVacuumdeliveryofsamplescanproduceinconsistentlysizeddropletsMonitordropletsizeduringgenerationandadjustpressureasnecessarytomaintainconsistentsizeConsistencyinprocessingisthebasisforconsistencyinresultsThermocyclingisbasedon96wellplatewhichremainsopenuntillastsamplesareloadedClosedtubedesigneliminatesthepotentialforcontaminationandspills.8samplestripdesignreduceswasteandspeedstimetofirstresultContaminationcontrolisimportantindigitalPCRRequiresproprietarymastermixWorkswithseveralmastermixesLeadingedgesciencedemandsflexibilityAppliedBiosystemsQuantStudio?12KFlexbyLifeTechnologies4Loadsamples,MM,andassayinto384wellplateforAccuFill,sealwithfoil,thencutPlace384wellplate,tips,andarrayinAccuFillPlaceinplatesealerandapplylid–90seconds!PlaceinholderScrewtosealFillwithimmersionfluidMergesampleinfooninstrumentAnalyzedatausingDigitalSuiteTotaltime~4.5hoursexcludinganalysisLoadintoinstrumentandrun~4hoursSellingAgainstQuantStudio12KFlexOpenArray5CompetitorRainDropSolutionTakeHome3072maximumpartitionsUpto10,000,000dropletsgenerated3072partitionsdon’tgiveyousinglemoleculecounting–relianceonPoissonstatisticstogenerateresultsUpto48pipettingstepspersampleOnepipettingsteppersampleMorepipetting,moretime,moremoney,moreerrorCapillaryfillingoftipsandarraycanfailtofillpartitionsMonitordropletsizeduringgenerationandadjustpressureasnecessarytomaintainconsistentsizeConsistencyinprocessingisthebasisforconsistencyinresultsOnceloadedarraysarefragile–samplecanevaporate,getflushedoutofpartitions,orgetjarredfree,andbreakdownifnotrunimmediatelySamplesareinsealedPCRtubes,cannotevaporateorspill,andcanbestoredupto3daysifnecessaryFlexibletimingwithnorushfitsbetterintodailylabroutinesRequiresproprietarymastermixWorkswithseveralmastermixesLeadingedgesciencedemandsflexibilityQuantStudio?3DDigitalPCRSystem6Mixsample,assay,mastermix,andpipetontochipPlaceontoloader,usesqueegeetodistributeacrosschipPourimmersionfluidonchip,snaplidintoplateTransferdatatocomputerInsertchipintoinstrumenttoread,~2minPlaceinthermalcycler~3.5hoursSetROX,FAM,andVICbaselinesFuturesoftwareforRareAlleleandAbsoluteQuantitationTotaltime~4.5hoursexcludinganalysisSellingAgainstQuantStudio3DDigitalPCRsystem7CompetitorRainDropSolutionTakeHomeUpto20,000partitionsUpto10,000,000dropletsgenerated20kpartitionsdon’tgiveyousinglemoleculecounting–relianceonPoissonstatisticstogenerateresultsPipetting/squeegeeloadingofsampledifficult,requiresloader,usescapillaryaction-Onepipettingsteppersample-Closedlooppressurecontrolallows

RainDropSourcetodropletizetheentiresampleineachlanesimultaneouslyMorepipetting,moretime,moremoney,moreerror.CapillaryfillingcanresultininconsistentsamplevolumesPouringimmersionfluidandsnappinglidcanflushsamplesoutofpartitionsSamplesareinsealedPCRtubes,cannotevaporateorspill,andcanbestoredupto3daysifnecessaryConsistencyinprocessingisthebasisforconsistencyinresultsMustsitatinstrumenttofeedchipsineverycoupleminutesWalkawayworkflowwithminimalhandsontimeAt20Kperchip3Dwouldrequire50chipstoget1,000,000datapoints,whichwouldtakenearly2hoursofhandsontimetoreadRequiresproprietarymastermixWorkswithseveralmastermixesLeadingedgesciencedemandsflexibilityInosticsBEAMingServiceInosticsBEAMingServiceWorkflow9IsolateDNAMultiplexPCRSingle-plexPCRHybridizationtoProbesSingleMoleculeAmpShakenEmulsionPCRTime(Service)<10daysFlowCytometryCountingWashSteps/PrepforCytometryBreakDropletsandWashBeadsSellingAgainstInosticsWorkflow10CompetitorRainDropSolutionTakeHomeSinglemoleculeamplificationinnon-uniformdropletsSinglemoleculeamplificationin5pluniformdropletsUniformdropletsensuresinglemoleculeamplificationLODsimilar?Manymanualworkflowsteps(someautomation?)Singlepipettingst