孫秋娟(咼于明)：不同來(lái)源銅、鋅、錳對(duì)產(chǎn)蛋雞的生產(chǎn)性能、蛋品質(zhì)、酶活及微量沉積的影響

不同來(lái)源銅、鋅、錳對(duì)產(chǎn)蛋雞的生產(chǎn)性能、蛋品質(zhì)、酶活及微量沉積的影響

EffectsofDifferentSourcesofCu/Zn/MnonPerformance,EggQuality,EnzymeActivityandMineralRetentioninLayingHens報(bào)告人孫秋娟Reporter:SunQiujuan導(dǎo)師咼于明教授Supervisor:ProfessorGuo

Yuming

中國(guó)農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院CollegeofAnimalScience&Technology,ChinaAgriculturalUniversity研究背景Background鋅、銅、錳為動(dòng)物各種生理過(guò)程的必要元素。Zinc,copperandmanganeseareessentialinallanimalsforvarietyofphysiologicalprocess（Richards,2007）.調(diào)節(jié)免疫、組織結(jié)構(gòu)完整、抗氧化、酶的組成等等。Immuneresponse,tissueintegrity,protectingfromoxidativedamage,formationofenzymes,etc.穩(wěn)定性及拮抗物StabilityandAntagonisms研究背景BackgroundKnownmineralinteractions(Miller,1979)Phytate有機(jī)微量來(lái)源

OrganicMineralsSources絡(luò)合物Complexes螯合物Chelates

蛋白鹽Proteinates富硒酵母SelenizedYeastMINTREXZn/Cu/Mn為兩個(gè)蛋氨酸羥基類(lèi)似物配體與一個(gè)金屬原子形成的螯合物。MINTREXZn/Cu/Mnisachelateoftwo2-hydroxy-4(methylthio)butanoic

acid(HMTBa)ligandsperatomoftracemineral.ZincAminoAcidchelate氨基酸螯合鋅研究背景Background有機(jī)微量元素在肉雞日糧中使用，其生物利用率要高于無(wú)機(jī)微量元素（Abdallah,2009；Peric,2008；Swiatkiewicz,2008等）。Organicmineralshavehigherbioavailabilityinbroilers.日糧中添加蛋氨酸可提高小鼠對(duì)鋅的吸收（House,1997）。Methionineinratdietcanaffectzincabsorption.試驗(yàn)?zāi)康腛bjective評(píng)定在剔除配體影響后，MintrexCu/Zn/Mn對(duì)蛋雞生產(chǎn)性能、蛋品質(zhì)及酶活、微量沉積等的影響。

AssesseffectsofdifferentCu/Zn/Mnsources(NovusInternationalInc.)withthesamelevelofmethionineonperformance,eggquality,enzymeacitivityandmineralretentionoflayinghens.材料和方法MaterialsandMethods試驗(yàn)采用315只37周齡海蘭褐蛋雞，隨機(jī)分成4個(gè)處理，每處理6個(gè)重復(fù)，每重復(fù)3籠，每籠3只雞。Atotalof31537wk-oldHylinebrownlayers,2treatments,6replicatespertreatment(3pensperreplicatewith3layerseach).Treatmentdiet日糧處理處理Cu（ppm）Zn（ppm）Mn（ppm）CuSO4MintrexCuZnSO4MintrexZnMnSO4Mintrex

MnT110-30-30-T2-1030-30-T310-102030-T410-30-1020材料和方法MaterialsandMethods各日糧能量、含氮量及蛋氨酸含量相同。Alldietswereiso-energetic,iso-nitrogenousandiso-methionine,partoftheMetsourcewasprovidedfromMintrex.ZincAminoAcidchelate氨基酸螯合鋅

Mintrex

Cu:Cu15%,Met78%;Mintrex

Zn:Zn16%,Met80%;

Mintrex

Mn:Mn13%,Met76%?；A(chǔ)日糧微量含量為：Cu6.5ppm，Mn17ppm，Zn20ppm。Tracemineralsinbasaldiets:Cu6.5ppm,Mn17ppm,Zn20ppm.預(yù)飼兩周，不添加微量元素。試驗(yàn)期為39周齡到48周齡。Twoweeksforpre-feedingwithnomineralssupplement.Theexperimentlastedfortenweeksfrom39to48wksofage.

測(cè)定指標(biāo)Determination生產(chǎn)性能Production產(chǎn)蛋率、采食量、蛋重、破蛋率Layingpercentage,Feedintake,Eggweight,Crackedpercentage蛋品質(zhì)EggQuality蛋殼厚度，蛋殼強(qiáng)度，ShellthicknessShellstrength存放10天后的蛋白高度、哈氏單位及蛋黃顏色酶活EnzymesActivityTreatment1&2:CuZn-SOD&Ceruloplasmin

銅鋅超氧化物歧化酶、銅藍(lán)蛋白Treatment1&3:Carbonicanhydrase&Alkalinephosphatase&CuZn-SOD

碳酸酐酶、堿性磷酸酶、CuZn-SODTreatment1&4:Catalase、Mn-SOD

過(guò)氧化氫酶、Mn-SOD微量沉積MineralRetention肝臟、胰臟、脾臟、脛骨、趾骨Liver,pancreas,spleen,tibia,phalanxStatisticalAnalysis統(tǒng)計(jì)分析使用SASv8統(tǒng)計(jì)軟件，與對(duì)照組用TTEST進(jìn)行顯著性檢驗(yàn)。AlldatawasanalyzedusingtheSASprogramversion8(SASInstitute).MeansofvariableswerecomparedusingTTEST.試驗(yàn)結(jié)果——銅Results-CopperControlMintrexCupvalue39-43wkEggWeight(g)平均蛋重61.3±0.961.5±2.40.8350LayingRate(%)雞日產(chǎn)蛋率90.4±2.991.5±2.60.4914CrackRate(%)破蛋率0.58±0.860.60±0.760.9591FCR（Feed：EggMass）料蛋比1.91±0.071.87±0.080.448EggMass(g)雞日產(chǎn)蛋量55.4±2.256.2±1.90.4801FeedIntake(g)采食量105.6±6.3104.3±3.10.670539-48wkEggWeight(g)61.7±0.962.0±2.20.7332Layingrate(%)90.1±0.890.8±0.90.5916CrackRate(%)0.44±0.270.40±0.270.7365FCR1.92±0.081.89±0.140.648EggMass(g)55.7±0.756.3±0.70.5455FeedIntake(g)107.5±6.5108.3±6.50.9459不同銅來(lái)源對(duì)生產(chǎn)性能的影響EffectsofCusourcesonproduction試驗(yàn)結(jié)果——銅Results-Copper不同銅來(lái)源對(duì)儲(chǔ)存10天后蛋品質(zhì)的影響EffectsofCusourcesoneggqualityafter10daysstorage

ControlMintrexCupvalue蛋黃顏色7.6±0.57.0±0.80.0256蛋白高度4.91±0.885.74±0.830.0132哈氏單位67.2±7.473.9±6.30.0163試驗(yàn)結(jié)果——銅Results-CopperControlMintrexCupvalue43周齡16.97±0.7823.39±1.590.007948周齡22.88±2.0734.45±2.100.0682不同銅來(lái)源對(duì)肝臟銅藍(lán)蛋白（CP）的影響EffectsofCusourcesonCPinliver試驗(yàn)結(jié)果——銅Results-Copperp<0.01p<0.10p<0.05p<0.01小結(jié)-銅Summary-CuMintrexCu等量代替硫酸銅可提高43周齡蛋黃中、48周齡肝臟及脾臟中銅水平。MintrexCutoreplaceCuSO4increasedCulevelinyolkatwk43,liverandspleenatwk48.銅藍(lán)蛋白活性也隨之升高。CP(ceruloplasmin)activitywasincreasedatwk48.減緩儲(chǔ)存對(duì)蛋品質(zhì)的不利影響。Slowdowneffectsofstorageoneggquality.試驗(yàn)結(jié)果——鋅Results-ZincControlMintrexZnpvalue39-43wkEggWeight(g)平均蛋重61.3±0.962.0±1.00.1659LayingRate(%)雞日產(chǎn)蛋率90.4±2.990.0±2.20.7322CrackRate(%)破蛋率0.58±0.860.44±0.520.5106FCR(Feed：EggMass)料蛋比1.91±0.071.86±0.230.604EggMass(g)雞日產(chǎn)蛋量55.4±2.255.8±1.20.681FeedIntake(g)采食量105.6±6.3103.4±12.40.689639-48wkEggWeight(g)61.7±0.962.5±0.80.1143Layingrate(%)90.1±0.889.9±0.90.8569CrackRate(%)0.44±0.270.73±0.290.464FCR1.92±0.081.89±0.080.786EggMass(g)55.7±0.756.2±0.80.5182FeedIntake(g)107.5±6.5106.7±10.20.961不同鋅來(lái)源對(duì)生產(chǎn)性能的影響EffectsofZnsourcesonproduction試驗(yàn)結(jié)果——鋅Results-Zinc不同鋅來(lái)源對(duì)儲(chǔ)存10天后蛋品質(zhì)的影響EffectsofZnsourcesoneggqualityafter10daysstorage

ControlMintrexZnpvalue蛋黃顏色7.6±0.56.9±0.60.0017蛋白高度4.91±0.885.88±0.970.0080哈氏單位67.2±7.473.0±7.30.0438試驗(yàn)結(jié)果——鋅Results-ZincControlMintrexZnpvalue43周齡0.48±0.090.62±0.070.018648周齡0.70±0.100.92±0.140.0196不同鋅來(lái)源對(duì)肝臟碳酸苷酶（CA）的影響EffectsofZnsourcesonCAactivityinliver試驗(yàn)結(jié)果——鋅Results-Zincp=0.06p<0.01p<0.01小結(jié)-鋅Summary-ZincMintrexZn部分替代硫酸鋅，43周齡蛋黃鋅水平提高，脛骨、脾臟鋅水平降低。Znlevelinyolkwereincreased,butdecreasedintibiaandspleenatwk43bypartiallyreplacingZnsulfatewithMintrexZn.肝臟碳酸苷酶活性提高。CAactivitiesinliveratwk48wereenhanced.減緩儲(chǔ)存對(duì)蛋品質(zhì)的不利影響。Slowdowneffectsofstorageoneggquality.試驗(yàn)結(jié)果——錳Results-ManganeseControlMintrex

Mnpvalue39-43wkEggWeight(g)平均蛋重61.3±0.962.3±2.10.282LayingRate(%)雞日產(chǎn)蛋率90.4±2.991.3±3.40.6055CrackRate(%)破蛋率0.58±0.860.42±0.530.942EggMass(g)雞日產(chǎn)蛋量55.4±2.256.9±3.60.388FCR（Feed：EggMass）料蛋比1.91±0.071.71±0.420.248FeedIntake(g)采食量105.6±6.396.4±21.50.401539-48wkEggWeight(g)61.7±0.963.7±2.20.035Layingrate(%)90.1±0.891.3±1.00.4267CrackRate(%)0.44±0.270.64±0.310.9404EggMass(g)55.7±0.757.5±0.90.2158FCR1.92±0.081.73±0.090.326FeedIntake(g)107.5±6.595.2±15.50.4752不同錳來(lái)源對(duì)生產(chǎn)性能的影響EffectsofMnsourcesonproduction試驗(yàn)結(jié)果——錳Results-ManganeseControlMintrex

Mnpvalue43wkShellThickness蛋殼厚度335.2±15.9342.9±20.40.1943ShellStrength蛋殼強(qiáng)度3.00±0.473.18±0.610.243348wkShellThickness334.4±24.0350.5±25.30.0171ShellStrength3.21±0.513.31±0.570.4865p<0.05不同錳來(lái)源對(duì)蛋殼質(zhì)量的影響EffectsofMnsourcesonshellquality試驗(yàn)結(jié)果——錳Results-Manganese不同錳來(lái)源對(duì)儲(chǔ)存10天后蛋品質(zhì)的影響EffectsofMnsourcesoneggqualityafter10daysstorage

ControlMintrex

Mnpvalue蛋黃顏色7.6±0.57.0±0.50.0038蛋白高度4.91±0.885.45±0.960.1242哈氏單位67.2±7.470.0±7.70.3303試驗(yàn)結(jié)果——錳Results-ManganeseControlMintrex

MnpvalueMn-SOD48.0±6.6108.6±2