金邊瑞香論文：金邊瑞香醇提物化學(xué)成分的分離、鑒定以及抗氧化活

1、金邊瑞香論文：金邊瑞香醇提物化學(xué)成分的分離、鑒定以及抗氧化活性研究【中文摘要】金邊瑞香(Daphne odora var. marginata系瑞香科(Thymelaeaceae瑞香屬(Daphne落葉常綠灌木,為瑞香的變種。產(chǎn)自被譽(yù)為“中國瑞香之鄉(xiāng)”江西省贛南大余縣的金邊瑞香是瑞香中的佳品,葉綠金黃色,花淡紫,花瓣先端5裂,白色,其基部紫紅,香味濃烈,以“色、香、姿、韻”四絕著稱于世,深受海內(nèi)外花卉愛好者的親睞,故有“牡丹花國色天香,瑞香花金邊最良”之說。用葉治咽喉腫痛、齒痛、血疔熱癤,用花治療無名腫毒及各種皮膚病。本文對金邊瑞香水醇相(70%乙醇提取物褐色固體析出物的化學(xué)成分和抗氧化活性進(jìn)

2、行了研究,以期尋找新結(jié)構(gòu)的化合物物或有生物活性的先導(dǎo)物,為金邊瑞香的開發(fā)利用提供進(jìn)一步的科學(xué)依據(jù)。第一章緒論部分,對金邊瑞香植物化學(xué)成分和不同組分生物活性研究概況進(jìn)行綜述；概述了近年來天然產(chǎn)物中黃酮類化學(xué)成分以及金邊瑞香中已知黃酮類化學(xué)成分的提取分離方法。第二章以金邊瑞香醇提物所得褐色固體析出物為研究對象,采用硅膠、ODS、Sephadex LH-20柱色譜,分離得到十個單體化合物。第三章對金邊瑞香中分離得到10個化合物的結(jié)構(gòu)采用核磁共振波譜、質(zhì)譜及紫外光譜等譜學(xué)數(shù)據(jù)進(jìn)行分析,并與相關(guān)文獻(xiàn)對照鑒定了他們的結(jié)構(gòu)：蕘花醇B (wikatrols B、山奈酚(kaempferol、瑞香黃烷B (da

3、phnodorin B瑞香黃烷D2 (daphnodorin D2、瑞香黃烷A (daphnodorin A、瑞香黃烷C(daphnodorin C、瑞香黃烷D1 (daphnodorin D1、瑞香黃烷I (daphnodorin I、瑞香素(daphnetin,雙白瑞香素(daphnoretin。第四章擬采用清除DPPH自由基能力,【英文摘要】Daphne odora var. marginata, family of Thymelaeaceae, is a famous flower and medical plant in the regions along the Yangzi Ri

4、ver. It has been used in Chinese traditional medicine for many diseases. Their leaves have been mashed up to kurashiki the affected part for the ulcerate, injury, and mastitis; Their flowers soaked by white spirit have been used to cure rheumatoid arthritis, rheumatism, carbuncle, furuncle, miliaria

5、, unknown toxin and traumatic injury. In this dissertation, the chemical constituents and antioxidant activity of the extracts from Daphne odora var. marginata were studied to find the main medicinal compounds with biological activity or novel structure. It would offer the theoretical foundation for

6、 the depth development and utilization of Daphne odora var. marginata.In the first chapter, we have described the progress of chemical constituents and pharmacological activity in Daphne odora var. marginata. Furthermore, the distribution and bioactivity of coumarin and daphnodorin which were charac

7、teristic components in Thymelaeaceae were reviewed.In the second chapter, the fractions was from Daphne odora var. marginata extracted by 70% ethanol, and then were subjected to column chromatography on silica gel, ODS and Sephadex LH-20. Ten compounds were separated and purified from this plant.In

8、the third chapter, the physical and chemical properties of the ten compounds were investigated by UV-vis, IR, ESI-MS, EI-MS,1H NMR and 13C NMR, as follow: wikatrols B, kaempferol, daphnodorin B, daphnodorin D2, daphnodorin A, daphnodorin C, daphnodorin D1, daphnodorin I, daphnetin, daphnoretin. This

9、 research showed great potential applications in medicinal plant.In the fourth chapter, the pertinence relation between structural and the antioxidant activities of nine compounds which separated and purified from this plant was respectively studied by the reducing power and scavenging activity of D

10、PPH radicals, total antioxidant activity, and OH- scavenging activity. Therefore, it would provide scientific foundation for curing disease related to radicals by using these compounds as safe, effective antioxidants or medicine.【關(guān)鍵詞】金邊瑞香 柱層析 抗氧化 蕘花醇B 山奈酚 瑞香黃烷B 瑞香黃烷D2 瑞香黃烷A 瑞香黃烷C 瑞香黃烷D1 瑞香黃烷I 瑞香素 雙白

11、瑞香素【英文關(guān)鍵詞】Daphne odora var. marginata column chromatography antioxidant activity wikatrols B kaempferol daphnodorin B daphnodorin D2 daphnodorin A daphnodorin C daphnodorin D1 daphnodorin I daphnetin daphnoretin【目錄】金邊瑞香醇提物化學(xué)成分的分離、鑒定以及抗氧化活性研究摘要3-4ABSTRACT4-5第一章 引言9-241.1 金邊瑞香概述9-101.2 金邊瑞香不同組分生物活性研究概

12、況10-121.2.1 金邊瑞香總浸膏生物活性研究10-111.2.2 金邊瑞香水提取物生物活性研究11-121.2.3 金邊瑞香有溶劑提取物生物活性研究121.2.4 金邊瑞香在臨床方面的效用121.3 化學(xué)成分方面的研究進(jìn)展12-151.3.1 類屬黃烷類的化合物12-131.3.2 類屬香豆素類以及木脂素類的化合物13-151.3.3 類屬其它類的化合物151.4 天然產(chǎn)物中黃酮類化學(xué)成分的提取分離概況15-191.4.1 黃酮類化合物的提取技術(shù)16-171.4.2 黃酮類化合物的分離技術(shù)17-191.5 金邊瑞香中已知黃酮類化合物分離與純化方法概述19-221.5.1 瑞香黃烷類化合物

13、的分離純化方法19-211.5.2 香豆素類化合物的分離純化方法211.5.3 木脂素類化合物的分離純化方法21-221.6 本課題研究的目的、意義22-241.6.1 課題來源221.6.2 研究意義22-24第二章 金邊瑞香水醇相提取物柱層析分離24-302.1 引言242.2 儀器、試劑及材料242.3 試驗(yàn)方法24-282.3.1 分離原理24-252.3.2 分離方法25-282.4 實(shí)驗(yàn)結(jié)果及討論282.5 結(jié)論28-30第三章 金邊瑞香水醇相提取物分離單體化合物結(jié)構(gòu)鑒定30-383.1 引言303.2 儀器、試劑及材料30-313.3 提取與分離313.4 單體化合相關(guān)數(shù)據(jù)313

14、.5 單體化合物的鑒定31-343.6 化合物的波譜數(shù)據(jù)34-373.7 結(jié)論37-38第四章 金邊瑞香中化合物的活性研究38-474.1 前言38-404.2 實(shí)驗(yàn)部分404.2.1 主要實(shí)驗(yàn)儀器404.2.2 主要實(shí)驗(yàn)藥品及試劑配置404.3 試驗(yàn)方法40-424.3.1 清除DPPH自由基實(shí)驗(yàn)40-414.3.2 原能力測定414.3.3 總抗氧化能力的測定414.3.4 清除羥基自由基能力41-424.4 結(jié)果與討論42-464.4.1 原FE3+能力42-434.4.2 清除DPPH自由基434.4.3 總抗氧化能力的測定43-444.4.4 清除羥基自由基能力44-454.4.5

15、討論45-464.5 結(jié)論46-47結(jié)論與展望47-48參考文獻(xiàn)48-54致謝54-55附錄55-75附錄A 瑞香黃烷A 1H-NMR譜圖55-56附錄B 瑞香黃烷B 1H-NMR譜圖56-57附錄C 瑞香黃烷B 13C-NMR譜圖57-58附錄D 瑞香黃烷C 1H-NMR譜圖58-59附錄E 瑞香黃烷C 13C-NMR譜59-60附錄F 瑞香黃烷D_2 1H-NMR譜圖60-61附錄G 瑞香黃烷I 1H-NMR譜圖61-62附錄H 瑞香黃烷I 13C-NMR譜圖62-63附錄I 雙白瑞香素1H-NMR譜圖63-64附錄J 雙白瑞香素13C-NMR譜圖64-65附錄K 雙白瑞香素ESI-MS譜圖65-66附錄L 蕘花醇B 13C-NMR譜圖66-