MPM-1-DataSheet-生命科學(xué)試劑-MCE

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEMPM-1Cat.No.:HY-151503分?式:C??H??F?N?O?分?量:734.73作?靶點:Autophagy作?通路:Autophagy儲存?式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY?物活性MPM-1海洋?物Eusynstyelamides模擬物，?種有效的抗癌劑。MPM-1可通過誘導(dǎo)壞死樣死亡，在體外快速殺死癌細(xì)胞。MPM-1具有誘導(dǎo)免疫原性細(xì)胞死亡的能?。MPM-1在癌細(xì)胞中?擾細(xì)胞?噬(autophagy)和溶酶體膨脹。IC50&TargetAutophagy[1]體外研究MPM-1(0-50μM;4h)hascytotoxicityagainstvarioushumancancercelllines[1].MPM-1(8.5and17.0μM;1-6h)causesperturbationofautophagyandlysosomalswelling[1].MPM-1inducesthereleaseandexposureofdamage-associatedmolecularpatterns(DAMPs)relatedtoimmunogeniccelldeath[1].CellCytotoxicityAssay[1]CellLine:Jurkat,Ramos,HSC-3,MCF-7,A375,etal.Concentration:0-50μMIncubationTime:4hResult:ExhibitedpotentcytotoxicityagainstJurkat,Ramos,HSC-3,MCF-7,A375withIC50sof6.62?±?1.60μM,7.53?±?2.01μM,8.53?±?0.57μM,14.06?±?2.71μM,14.52?±?0.22μM,respectively.Immunofluorescence[1]1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemECellLine:HSC-3Concentration:8.5and17.0μMIncubationTime:1,2,4and6hResult:Significantlyincreasedthetotalnumberofautophagymarkersp62(substrateofautophagy)andLC3B.Causedthedistributionofthelysotrackerdyemorediffuseandlessintense,indicatingthatlysosomalmorphologywasinfluenced.WesternBlotAnalysis[1]CellLine:RamosandHSC-3Concentration:8.5μMforRomasand17.0μMforHSC-3IncubationTime:0.5,1,2,3and4hResult:Increasedthereleaseofhighmobilitygroupbox1(HMGB1)fromcells.REFERENCES[1].SusannahvonHofsten,etal.ThemarinenaturalproductmimicMPM-1iscytolyticandinducesDAMPreleasefromhumancancercelllines.SciRep.2022Sep16;12(1):15586.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3