Anti-inflammatory-agent-36-DataSheet-生命科學(xué)試劑-MCE

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEAnti-inflammatoryagent36Cat.No.:HY-148553CASNo.:2293951-01-4分?式:C??H??NO?分?量:453.48作?靶點:Others作?通路:Others儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:25mg/mL(55.13mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.2052mL11.0258mL22.0517mL5mM0.4410mL2.2052mL4.4103mL10mM0.2205mL1.1026mL2.2052mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥1.25mg/mL(2.76mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性Anti-inflammatoryagent36?種抗炎劑。Anti-inflammatoryagent36抑制LPS誘導(dǎo)的巨噬細胞活化。體外研究Anti-inflammatoryagent36(Compound5a28)(2.5-20μM)inhibitsLPS-inducedreleaseofTNF-αandIL-6inactiveRAW264.7mousemacrophages,withIC50sof3.69and3.68μMforTNF-αandIL-6[1].Anti-inflammatoryagent36(10μM,0.5h)inhibitsLPS-inducedP-P38andP-ERKinRAW264.7mousemacrophages[1].Anti-inflammatoryagent36(10μM,0.5h)inhibitsLPS-inducedthetranscriptionofTNF-α,IL-6,IL-1β,ICAM-1andVCAM-1[1].WesternBlotAnalysis[1]CellLine:RAW264.7mousemacrophagesConcentration:10μMIncubationTime:0.5hResult:MarkedlyinhibitedP-P38andP-ERK,indicatingthesuppressionofMAPKsignaling.體內(nèi)研究Anti-inflammatoryagent36(Compound5a28)(10mg/kg,i.p.)inhibitsinflammationinacutelunginjurymousemodel[1].AnimalModel:Acutelunginjurymousemodel[1]Dosage:10mg/kgAdministration:i.p.Result:Reducedwet/dryweightratioofthemicelungs.Reducedbiomarkersoflymphocytesandmacrophages.SuppressesTNF-α,IL-6,IL-1β,7VACM-1andICAM-1level.REFERENCES[1].QianJ,etal.Designandsynthesisnoveldi-carbonylanalogsofcurcumin(DACs)actaspotentanti-inflammatoryagentsagainstLPS-inducedacutelunginjury(ALI).EurJMedChem.2019Apr1;167:414-425.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearch