VH032-PEG5-C6-Cl-HaloPROTAC-2-DataSheet-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEVH032-PEG5-C6-ClCat.No.:HY-112495CASNo.:1799506-06-1Synonyms:HaloPROTAC2分?式:C??H??ClN?O?S分?量:783.41作?靶點:E3LigaseLigand-LinkerConjugates作?通路:PROTAC儲存?式:-80°C,protectfromlight,storedundernitrogen溶解性數(shù)據(jù)體外實驗DMSO:270mg/mL(344.65mM;Needultrasonic)Ethanol:100mg/mL(127.65mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.2765mL6.3824mL12.7647mL5mM0.2553mL1.2765mL2.5529mL10mM0.1276mL0.6382mL1.2765mL請根據(jù)產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。儲備液的保存?式和期限：-80°C,6months(protectfromlight,storedundernitrogen)。-80°C儲存時，請在6個?內使?。體內實驗請根據(jù)您的實驗動物和給藥?式選擇適當?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的?作液，建議您現(xiàn)?現(xiàn)配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥5mg/mL(6.38mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥5mg/mL(6.38mM);Clearsolution3.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥5mg/mL(6.38mM);ClearsolutionBIOLOGICALACTIVITY?物活性VH032-PEG5-C6-Cl(HaloPROTAC2)由E3配體與21個原?相連的復合分?，連接?的接頭為Halogen團。VH032-PEG5-C6-Cl(HaloPROTAC2)包含于VH032的VHL配體和5個單元PEGlinker。VH032-PEG5-C6-Cl能夠誘導GFP-HaloTag7降解[1]。IC50&TargetVHL體外研究Treatment(24hour)withVH032-PEG5-C6-Clleadstonearly70%degradationofGFP-Halotag7at2.5μM[1].REFERENCES[1].BuckleyDL,etal.HaloPROTACS:UseofSmallMoleculePROTACstoInduceDegradationofHaloTagFusionProteins.ACSChemBiol.2015Aug21;10(8):1831-7.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;